US5646039A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Microfabricated reactor
Assignee: University of California, San Diego
•
Filing: 1995-06-06
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Granted: 1997-07-08
•
Expires: 2015-06-06
Abstract
Abstract An integrated microfabricated instrument for manipulation, reaction and detection of microliter to picoliter samples. The instrument is suited for biochemical reactions, particularly DNA-based reactions such as the polymerase chain reaction, that require thermal cycling since the inherently small size of the instrument facilitates rapid cycle times. The integrated nature of the instrument provides accurate, contamination-free processing. The instrument may include reagent reservoirs, agitators and mixers, heaters, pumps, and optical or electromechanical sensors. Ultrasonic Lamb-wave devices may be used as sensors, pumps and agitators.
Inventors
- 👤 M. Allen Northrup
- 👤 Richard M. White
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 17:05
CPC Classifications
B; B01; B01L; B01L7/00; B01L7/52; B01F; B01F31/00; B01F31/80; B01F31/86; B01F33/00; B01F33/30; B01J; B01J19/00; B01J19/0093; B01L3/00; B01L3/50; B01L3/502; B01L3/5027; B01L3/502707; B01L3/50273; B01L7/525; G; G01; G01N; G01N29/00; G01N29/02; G01N29/022; G01N35/00; B01J2219/00; B01J2219/00781; B01J2219/00819; B01J2219/00824; B01J2219/00828; B01J2219/00831; B01J2219/00873; B01J2219/00889; B01J2219/00891; B01J2219/00925; B01J2219/0093; B01J2219/00932; B01J2219/00934; B01J2219/0095; B01J2219/00952; B01L2300/00; B01L2300/18; B01L2300/1805; B01L2300/1827; B01L2400/00; B01L2400/04; B01L2400/0403; B01L2400/0406; B01L2400/0433; B01L2400/0436; B01L9/00; B01L9/52; B01L9/527; G01N2035/00178; G01N2035/00237; G01N2035/00465; G01N2035/00534; G01N2035/00554; G01N2291/00; G01N2291/02; G01N2291/024; G01N2291/02466; G01N2291/025; G01N2291/0256; G01N2291/04; G01N2291/042; G01N2291/0421; G01N35/10; G01N35/1079; Y; Y10; Y10S; Y10S73/00; Y10S73/04
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
92 words
A microfabricated reactor, comprising: a substrate defining a chamber in which a measurement of viscosity may occur corresponding to a parameter of a reaction, said chamber having a thin film wall; an element microfabricated on said thin film wall for manipulation of a property of said reaction; at least one Lamb-wave transducer located on another wall of said chamber; such that said at least one Lamb-wave transducer measures the viscosity of contents of said chamber corresponding to said parameter, thereby preventing boundary layer effects from reducing the effectiveness of said viscosity measurement.
Claim 2
Dependent Claim
92 words
A microfabricated reactor, comprising: a substrate defining a chamber in which a measurement of viscosity may occur corresponding to a parameter of a reaction, said chamber having a thin film wall; an element microfabricated on said thin film wall for manipulation of a property of said reaction; at least one Lamb-wave transducer located on another wall of said chamber; such that said at least one Lamb-wave transducer measures the viscosity of contents of said chamber corresponding to said parameter, thereby preventing boundary layer effects from reducing the effectiveness of said viscosity measurement.
Claim 3
Dependent Claim
20 words
The reactor of claim 1, wherein said microfabricated element is a heater and said parameter of said measurement is temperature.
Claim 4
Dependent Claim
13 words
The reactor of claim 1, wherein said parameter of said measurement is density.
Claim 5
Dependent Claim
15 words
The reactor of claim 1, wherein said parameter of said measurement is a reagent concentration.
Claim 6
Dependent Claim
15 words
The reactor of claim 1, wherein said parameter of said measurement is a product concentration.
Claim 7
Dependent Claim
73 words
A microfabricated mixing chamber defined by at least two substantially opposing walls, comprising at least one Lamb-wave transducer located on each of said substantially opposing walls, said Lamb-wave transducer on one opposing wall propagating Lamb-waves in one direction, and said Lamb-wave transducer on the other substantially opposing wall propagating Lamb-waves in a substantially opposite direction to stir contents of said chamber, thereby preventing boundary layer effects from reducing the effectiveness of said stirring.
Claim 8
Dependent Claim
73 words
A microfabricated mixing chamber defined by at least two substantially opposing walls, comprising at least one Lamb-wave transducer located on each of said substantially opposing walls, said Lamb-wave transducer on one opposing wall propagating Lamb-waves in one direction, and said Lamb-wave transducer on the other substantially opposing wall propagating Lamb-waves in a substantially opposite direction to stir contents of said chamber, thereby preventing boundary layer effects from reducing the effectiveness of said stirring.
References Cited (Backward Citations)
US2127193A
Cited
US3029743A
Cited
US4219335A
Cited
US4556467A
Cited
US4632808A
Cited
US5220189A
Cited
US4673657A
Cited
US4598049A
Cited
US4708931A
Cited
US4596697A
Cited
US5000817A
Cited
US4602184A
Cited
US4692654A
Cited
US4676274A
Cited
US4963498A
Cited
EP0483117A2
Cited
US4737464A
Cited
US4683195A
Cited
US4683195B1
Cited
US4759828A
Cited
US4952266A
Cited
US4920056A
Cited
US5129262A
Cited
US5212988A
Cited
JPH01280694A
Cited
US5252294A
Cited
EP0347579A2
Cited
US4960486A
Cited
US4908112A
Cited
EP0385964A2
Cited
EP0381501B1
Cited
US5229297A
Cited
EP0402995A2
Cited
FR2650657A1
Cited
US5003822A
Cited
US5006749A
Cited
WO1991016966A1
Cited
US5270183A
Cited
US5153476A
Cited
US5385709A
Cited
US5304487A
Cited
US5296375A
Cited
WO1993022058A1
Cited
US5427946A
Cited
US5486335A
Cited
US5498392A
Cited
US5384029A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-07-07 | 2001-01-06 | 2001-07-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-07-07 | 2005-01-06 | 2005-07-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-07-07 | 2009-01-06 | 2009-07-08 | $7700.00 | Window Closed / Lapsed |