US5633724A — Evanescent scanning of biochemical array

Claim 1

Independent Claim 99 words

An apparatus for detecting a target substance in a pixel array comprising: (a) a light source to emit a light suitable for exciting the target substance; (b) a total internal reflection (TIR) member having a TIR surface on which a pixel array is located, the light from the light source passing into the TIR member and is reflected by the TIR surface, the pixel array being within an evanescent field region at the TIR surface; and (c) a light detector for detection of light emitted from the pixel array as a result of evanescent excitation of the target substance.

Claim 2

Independent Claim 99 words

An apparatus for detecting a target substance in a pixel array comprising: (a) a light source to emit a light suitable for exciting the target substance; (b) a total internal reflection (TIR) member having a TIR surface on which a pixel array is located, the light from the light source passing into the TIR member and is reflected by the TIR surface, the pixel array being within an evanescent field region at the TIR surface; and (c) a light detector for detection of light emitted from the pixel array as a result of evanescent excitation of the target substance.

Claim 3

Dependent Claim 46 words

The apparatus of claim 1 further comprising an optical resonator that has a resonance cavity for the light generated by the light source wherein the TIR member is located within the resonance cavity such that light traveling within the resonance cavity impinges on the TIR surface.

Claim 4

Dependent Claim 23 words

The apparatus of claim 2 wherein the light source is a gain medium of a laser and is located outside the resonance cavity.

Claim 5

Dependent Claim 14 words

The apparatus of claim 2 wherein the light source is an antireflection-coated semiconductor laser.

Claim 6

Dependent Claim 21 words

The apparatus of claim 1 wherein the light from the light source is reflected substantially without loss by the TIR surface.

Claim 7

Dependent Claim 18 words

The apparatus of claim 1 wherein the light source irradiates all the pixels of the pixel array simultaneously.

Claim 8

Dependent Claim 14 words

The apparatus of claim 1 wherein the TIR member is stationary during light detection.

Claim 9

Dependent Claim 14 words

The apparatus of claim 1 wherein the light detector is motionless during light detection.

Claim 10

Dependent Claim 57 words

The apparatus of claim 1 further comprising a prism and a refractive index match/ng film wherein the refractive index matching film is disposed between the TIR member and the prism such that the light from the light source passes through the prism, the refractive index matching layer and the TIR member before impinging on the TIR surface.

Claim 11

Dependent Claim 15 words

The apparatus of claim 1 wherein the light detector is a two dimensional array detector.

Claim 12

Dependent Claim 26 words

The apparatus of claim 1 wherein the apparatus further comprises an optical filter and optical fibers for transferring light from the optical filter to the detector.

Claim 13

Dependent Claim 22 words

The apparatus of claim 1 wherein pixels in the pixel array are pixels having polymeric sections for coupling with polymeric target substances.

Claim 14

Independent Claim 134 words

A method of analyzing a pixel array of chemical molecules for a target substance, comprising: (a) contacting a chemical array with a sample suspected of containing a target substance to form the pixel array; (b) directing light from a light source to pass into a total internal reflection (TIR) member having a TIR surface and an array surface on which the pixel array is located to result in an evanescent field region such that the pixel array is within the evanescent field region at the TIR surface, thereby causing excitation of the target substance on the pixel array, the light from the light source being reflected from the TIR surface; and (c) detecting light emitted from the pixel array as a result of the excitation of the target substance caused by the evanescent field.

Claim 15

Independent Claim 134 words

A method of analyzing a pixel array of chemical molecules for a target substance, comprising: (a) contacting a chemical array with a sample suspected of containing a target substance to form the pixel array; (b) directing light from a light source to pass into a total internal reflection (TIR) member having a TIR surface and an array surface on which the pixel array is located to result in an evanescent field region such that the pixel array is within the evanescent field region at the TIR surface, thereby causing excitation of the target substance on the pixel array, the light from the light source being reflected from the TIR surface; and (c) detecting light emitted from the pixel array as a result of the excitation of the target substance caused by the evanescent field.

Claim 16

Dependent Claim 41 words

The method of claim 13 further comprising mounting the TIR member inside an optical resonator that has a resonance cavity for the light generated by the light source such that light traveling within the resonance cavity impinges on the TIR surface.

Claim 17

Dependent Claim 21 words

The method of claim 13 wherein the light from the light source is reflected by the TIR surface substantially without loss.

Claim 18

Dependent Claim 17 words

The method of claim 13 wherein the light source irradiates all pixels of the pixel array simultaneously.

Claim 19

Dependent Claim 14 words

The method of claim 13 wherein the TIR member is stationary during light detection.

Claim 20

Dependent Claim 14 words

The method of claim 13 wherein the light detector is motionless during light detection.

Claim 21

Independent Claim 147 words

A method of making an apparatus for detecting a target substance on a pixel array comprising: (a) mounting a total internal reflection (TIR) member on a support, the TIR member having a TIR surface on which the pixel array can be located, the pixel array being capable of binding the target substance; (b) mounting a light source on the support to irradiate a light suitable for exciting the target substance on a pixel array located on the TIR surface such that the light from the light source passes into the TIR member and is reflected by the TIR surface to result in an evanescent field region, the pixel array being within the evanescent field region at the TIR surface; and (c) mounting a light detector on the support for detecting light emitted from the pixel array as a result of the evanescent excitation of the target substance.

Claim 22

Independent Claim 147 words

A method of making an apparatus for detecting a target substance on a pixel array comprising: (a) mounting a total internal reflection (TIR) member on a support, the TIR member having a TIR surface on which the pixel array can be located, the pixel array being capable of binding the target substance; (b) mounting a light source on the support to irradiate a light suitable for exciting the target substance on a pixel array located on the TIR surface such that the light from the light source passes into the TIR member and is reflected by the TIR surface to result in an evanescent field region, the pixel array being within the evanescent field region at the TIR surface; and (c) mounting a light detector on the support for detecting light emitted from the pixel array as a result of the evanescent excitation of the target substance.

Annuity Stage	Window Opens	Payment Deadline	Surcharge Period Until	Estimated Fee	Status
3.5 Years (Year 4)	2000-05-26	2000-11-25	2001-05-27	$2000.00	Window Closed / Lapsed
7.5 Years (Year 8)	2004-05-26	2004-11-25	2005-05-27	$3760.00	Window Closed / Lapsed
11.5 Years (Year 12)	2008-05-26	2008-11-25	2009-05-27	$7700.00	Window Closed / Lapsed

Evanescent scanning of biochemical array

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