US5624537A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Biosensor and interface membrane
Assignee: University of British Columbia
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Filing: 1994-09-20
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Granted: 1997-04-29
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Expires: 2014-09-20
Abstract
Abstract A regenerable biosensor probe adapted for positioning in a bioreactor comprises a selectively permeable interface membrane, a porous protein-receiving matrix adjacent to the interface membrane, an indicating electrode, an inlet conduit through which fresh protein conjugate may flow to the protein-receiving matrix, and an outlet conduit through which spent protein conjugate may be removed from the protein-receiving matrix. A selectively permeable interface membrane, which may be used in a biosensor system to separate biochemical, optical or other processes from an analyte matrix comprises a supporting mesh, a perfluorosulfonic acid polymer impregnated substrate and a homogenous film of perfluorosulfonic acid polymer. A method of preparing this interface membrane comprises fixing a substrate on to a supporting mesh to form a substrate membrane, casting a perfluorosulfonic and polymer on the substrate membrane and curing the product to so formed.
Inventors
- 👤 Robin F. B. Turner
- 👤 Douglas G. Kilburn
- 👤 Michael R. Phelps
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 19:02
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/002; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
49 words
A biosensing system including a selectively permeable interface membrane which is separate and distinct from an electrode and protein receiving matrix, and which is used to separate biochemical, optical or other processes from an analyte matrix, said membrane comprising a supporting mesh and a perfluorosulfonic acid polymer impregnated substrate.
Claim 2
Dependent Claim
49 words
A biosensing system including a selectively permeable interface membrane which is separate and distinct from an electrode and protein receiving matrix, and which is used to separate biochemical, optical or other processes from an analyte matrix, said membrane comprising a supporting mesh and a perfluorosulfonic acid polymer impregnated substrate.
Claim 3
Dependent Claim
25 words
A biosensing system of claim 1 wherein the substrate is selected from the group consisting of cellulose, cellulose acetate, nitrocellulose, polysulfone, polyvinylchloride, polyurethane and polyvinylalcohol.
Claim 4
Dependent Claim
12 words
A biosensing system of claim 1 wherein the substrate is cellulose triacetate.
Claim 5
Independent Claim
68 words
A method of preparing a selectively permeable interface membrane in a biosensor system which is separate and distinct from an electrode and protein-receiving matrix, and which is used to separate biochemical, optical or other processes from an analyte matrix which comprises: fixing a substrate onto a supporting mesh to form a substrate membrane; casting a perfluorosulfonic acid polymer on the substrate membrane; and curing the product so formed.
Claim 6
Independent Claim
68 words
A method of preparing a selectively permeable interface membrane in a biosensor system which is separate and distinct from an electrode and protein-receiving matrix, and which is used to separate biochemical, optical or other processes from an analyte matrix which comprises: fixing a substrate onto a supporting mesh to form a substrate membrane; casting a perfluorosulfonic acid polymer on the substrate membrane; and curing the product so formed.
Claim 7
Dependent Claim
24 words
The method of claim 4 wherein the substrate is selected from the group consisting of cellulose, cellulose acetate, nitrocellulose, polysulfone, polyvinylchloride, polyurethane and polyvinylalcohol.
Claim 8
Dependent Claim
11 words
The method of claim 4 wherein the substrate is cellulose triacetate.
Claim 9
Dependent Claim
26 words
The method of claim 4 wherein the supporting mesh is a metallic screen selected from the group consisting of stainless steel, aluminum, copper, silver and gold.
Claim 10
Dependent Claim
24 words
The method of claim 4 wherein the supporting mesh is a polymeric screen selected from the group consisting of poly(vinylchloride), poly(tetrafluoroethylene), polystyrene and polycarbonate.
Claim 11
Dependent Claim
14 words
The method of claim 4 wherein the supporting mesh is a fibrous filter material.
Claim 12
Dependent Claim
118 words
A regenerable biosensor probe for positioning in an environment characterized by the presence of biological molecules which are substrates for or products produced by enzymes in order to determine the presence of said molecules, said biosensor probe comprising: a selectively permeable interface membrane which separates the biochemical and electrochemical processes from the environment when the probe is in place; a porous protein-receiving matrix adjacent to the interface membrane; an indicating electrode covered with an electrically insulative material, said electrode abutting, at one of its ends, the protein-receiving matrix; an inlet conduit through which fresh protein conjugate may flow to the protein-receiving matrix; and an outlet conduit through which spent protein conjugate may be removed from the protein-receiving matrix.
Claim 13
Dependent Claim
118 words
A regenerable biosensor probe for positioning in an environment characterized by the presence of biological molecules which are substrates for or products produced by enzymes in order to determine the presence of said molecules, said biosensor probe comprising: a selectively permeable interface membrane which separates the biochemical and electrochemical processes from the environment when the probe is in place; a porous protein-receiving matrix adjacent to the interface membrane; an indicating electrode covered with an electrically insulative material, said electrode abutting, at one of its ends, the protein-receiving matrix; an inlet conduit through which fresh protein conjugate may flow to the protein-receiving matrix; and an outlet conduit through which spent protein conjugate may be removed from the protein-receiving matrix.
Claim 14
Dependent Claim
22 words
The biosensor probe of claim 10 wherein the protein conjugate comprises the cellulose binding domain extracted from bacteria of the genus Cellulomonas.
Claim 15
Dependent Claim
20 words
The biosensor probe of claim 10, wherein the interface membrane comprises a supporting mesh and a perfluorosulfonic acid impregnated substrate.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-04-28 | 2000-10-28 | 2001-04-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-04-28 | 2004-10-28 | 2005-04-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-04-28 | 2008-10-28 | 2009-04-29 | $7700.00 | Window Closed / Lapsed |