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US5622848A Expired / Lapsed US ⏱️ 0.0 years remaining

Electrically hydrolyzed salines as microbiocides for in vitro treatment of contaminated fluids containing blood

Assignee: ReOxcyn Discoveries Group Inc
•
Filing: 1994-07-15
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Granted: 1997-04-22
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Expires: 2014-07-15
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Abstract

Abstract A microbiocidal solution for in vivo and in vitro treatment of microbial infections includes an electrolyzed saline containing regulated amounts of ozone and active chlorine species wherein the ozone content is between about 5 and 100 mg/L and the active chlorine species content of between about 5 and 300 ppm. The active chlorine species comprises free chlorine, hypochlorous acid and the hypochlorite ion as measured by a chlorine selective electrode. The solution is prepared by subjecting a 1% or less saline to electrolysis under conditions sufficient to produce the desired active ingredients. The solution is preferably utilized at an isotonic saline concentration and may be adjusted with hypertonic saline. The solution may be used for the in vitro treatment of infected whole blood, blood cells or plasma to reduce contamination and is effective in treatment of fluids infected with HIV, hepatitis and other viral, bacterial and fungal agents. The solution may also be administered to warm blooded animals, including humans, by intravenous injection or other modes for similar purposes. If desired neutralizing agents, such as antioxidants, may be administered in correlation with the solution.

Inventors

  • 👤 Robert E. Morrow

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-24 03:02
CPC Classifications
C; C12; C12N; C12N7/00; A; A23; A23L; A23L29/00; A23L29/30; A23L29/37; A61; A61K; A61K31/00; A61K31/70; A61K31/715; A61K33/00; A61K33/40; A61K33/42; A61K35/00; A61K35/02; A61K35/08; A61K38/00; A61K38/04; A61K38/06; A61K38/063; A61K38/16; A61K38/43; A61K38/44; A61K38/446; A61K8/00; A61K8/18; A61K8/30; A61K8/33; A61K8/34; A61K8/345; A61K8/60; A61K8/72; A61K8/73; A61K8/732; A61L; A61L2/00; A61L2/16; A61L2/18; A61P; A61P31/00; A61P31/04; A61P31/10; A61P31/12; A61P31/14; A61P31/16; A61P31/20; A61P31/22; A61P33/00; A61P37/00; A61P37/02; A61P37/04; A61Q; A61Q11/00; A61Q19/00; C02; C02F; C02F1/00; C02F1/46; C02F1/461; C02F1/46104; C02F1/4618; C02F1/467; C02F1/4672; C07; C07C; C07C31/00; C07C31/18; A61L2103/00; A61L2103/05; C02F1/4674; C02F2103/00; C02F2103/02; C02F2103/026; C02F2303/00; C02F2303/04; C12N2740/00; C12N2740/00011; C12N2740/10011; C12N2740/16011; C12N2740/16061; C12N2740/16063; Y; Y02; Y02A; Y02A50/00; Y02A50/30; Y10; Y10S; Y10S435/00; Y10S435/817

Claims Analysis

Total Claims: 13
Claim 1
Independent Claim 97 words
A method for the in vitro treatment of a microbially contaminated fluid selected from the group consisting of whole blood, blood cells, blood plasma, and mixtures thereof which comprises contacting said fluid with an effective amount of a microbiocidal solution comprising an electrolyzed saline containing ozone and active chlorine species wherein the ozone content is in the range of about 5 and 100 mg/L and the active chlorine species content is in the range of about 5 and 300 ppm for a time sufficient to reduce the microbial contamination in said fluid while maintaining therapeutic characteristics thereof.
Claim 2
Independent Claim 97 words
A method for the in vitro treatment of a microbially contaminated fluid selected from the group consisting of whole blood, blood cells, blood plasma, and mixtures thereof which comprises contacting said fluid with an effective amount of a microbiocidal solution comprising an electrolyzed saline containing ozone and active chlorine species wherein the ozone content is in the range of about 5 and 100 mg/L and the active chlorine species content is in the range of about 5 and 300 ppm for a time sufficient to reduce the microbial contamination in said fluid while maintaining therapeutic characteristics thereof.
Claim 3
Dependent Claim 77 words
The method of claim 1 wherein said microbially contaminated fluid is contaminated by an agent selected from the group consisting of Epstein-Barr virus, hepatitis A, B or C virus, rhinovirus, rubeola virus, rubella virus, parvovirus, papilloma virus, influenza viruses, parainfluenza viruses, enteroviruses, Herpes simplex viruses, Varicella-zoster viruses, Adenoviruses, respiratory syncytial viruses, alphaviruses, flaviviruses, retroviruses, and agents of bacteremia, septicemia, fungal infections, parasitic infections, mycobacterial infections, Bacterial Gram positive infections, Bacterial Gram negative infections, superficial and systemic infections.
Claim 4
Dependent Claim 14 words
The method of claim 2 wherein said microbiocidal solution is an isotonic saline solution.
Claim 5
Dependent Claim 13 words
The method of claim 3 wherein said microbially contaminated fluid is whole blood.
Claim 6
Dependent Claim 13 words
The method of claim 3 wherein said microbially contaminated fluid is blood cells.
Claim 7
Dependent Claim 13 words
The method of claim 3 wherein said microbially contaminated fluid is blood plasma.
Claim 8
Dependent Claim 13 words
The method of claim 3 wherein said microbially contaminated fluid is retrovirus contaminated.
Claim 9
Dependent Claim 10 words
The method of claim 7 wherein said retrovirus is HIV.
Claim 10
Dependent Claim 13 words
The method of claim 8 wherein said microbially contaminated fluid is whole blood.
Claim 11
Dependent Claim 13 words
The method of claim 8 wherein said microbially contaminated fluid is blood cells.
Claim 12
Dependent Claim 13 words
The method of claim 8 wherein said microbially contaminated fluid is blood plasma.
Claim 13
Dependent Claim 16 words
The method of claim 3 wherein said microbially contaminated fluid is contaminated by a hepatitis virus.

References Cited (Backward Citations)

JPS5587712A Cited
US4236992A Cited
US4316787A Cited
JPS5632422A Cited
US4970216A Cited
US4976959A Cited
US4968616A Cited
US5028588A Cited
JPH01259001A Cited
US5507932A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 2000-04-21 2000-10-21 2001-04-22 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 2004-04-21 2004-10-21 2005-04-22 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 2008-04-21 2008-10-21 2009-04-22 $7700.00 Window Closed / Lapsed

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