US5614622A
Expired / Lapsed
US
⏱️ 0.0 years remaining
5-pentenoyl moiety as a nucleoside-amino protecting group, 4-pentenoyl-protected nucleotide synthons, and related oligonucleotide syntheses
Assignee: Aceragen Inc
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Filing: 1995-08-24
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Granted: 1997-03-25
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Expires: 2015-08-24
Abstract
Abstract The invention provides new methods for synthesizing oligonucleotides that allow for deprotection of the oligonucleotide under more mild conditions than existing methods. The invention further provides a nucleoside base protective group that is stable under oligonucleotide synthesis conditions, but which can be removed under more mild conditions than existing protective groups, as well as nucleoside synthons having such base protective groups.
Inventors
- 👤 Radhakrishnan P. Iyer
- 👤 Theresa Devlin
- 👤 Ivan Habus
- 👤 Dong Yu
- 👤 Sudhir Agrawal
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 22:55
CPC Classifications
C; C07; C07H; C07H19/00; C07H19/02; C07H19/04; C07H19/16; C07H19/06; C07H19/10; C07H19/20; C07H21/00; Y; Y02; Y02P; Y02P20/00; Y02P20/50; Y02P20/55
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
36 words
A nucleoside base protecting group attached to a nucleoside base, the protecting group having the structure ##STR5## wherein B is a nucleoside base and the nitrogen displayed is the protected amino moiety of the nucleoside base.
Claim 2
Dependent Claim
36 words
A nucleoside base protecting group attached to a nucleoside base, the protecting group having the structure ##STR5## wherein B is a nucleoside base and the nitrogen displayed is the protected amino moiety of the nucleoside base.
Claim 3
Dependent Claim
23 words
A nucleoside synthon which is a nucleoside phosphoramidite, a nucleoside phosphotriester, or a nucleoside H-phosphonate having a base-protecting group according to claim 1.
Claim 4
Dependent Claim
18 words
A method for synthesizing an oligonucleotide, the method comprising coupling together two nucleoside synthons according to claim 2.
Claim 5
Dependent Claim
29 words
A method for synthesizing an oligonucleotide, the method comprising coupling a nucleoside synthon according to claim 2 to a nucleoside that is covalently bound to a suitable solid support.
Claim 6
Dependent Claim
52 words
A method for synthesizing an oligonucleotide, the method comprising coupling a nucleoside synthon according to claim 2 to a nucleoside and deprotecting the nucleoside base with a reagent comprising Br 2 or I 2 in water, or in pyridine/ROH, wherein R is an alkyl, aralkyl or aryl group having 1-10 carbon atoms.
References Cited (Backward Citations)
US4415732A
Cited
US5149798A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-03-24 | 2000-09-23 | 2001-03-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-03-24 | 2004-09-23 | 2005-03-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-03-24 | 2008-09-23 | 2009-03-25 | $7700.00 | Window Closed / Lapsed |