US5607830A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for the continuous conditioning of a cell suspension
Assignee: Fresenius SE and Co KGaA
•
Filing: 1995-02-09
•
Granted: 1997-03-04
•
Expires: 2015-02-09
Abstract
Abstract A method for the continuous conditioning of a cell suspension, more particularly whole blood, in order to obtain an erythrocyte concentrate which is returned to the patient by autotransfusion. In accordance with the invention there are three treatment stages, that is to say sedimentation of the cell suspension, the addition of washing solution to the cell concentrate and renewed sedimentation and separation of the washing solution.
Inventors
- 👤 Wolfgang Biesel
- 👤 Bernd Mathieu
- 👤 Wolfram Weber
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 13:16
CPC Classifications
A; A61; A61M; A61M1/00; A61M1/36; A61M1/3693; A61M1/3692; A61M1/3696; B; B04; B04B; B04B5/00; B04B5/04; B04B5/0442; A61M2202/00; A61M2202/04; A61M2202/0413; A61M2202/0429; B04B2005/045; Y; Y10; Y10T; Y10T436/00; Y10T436/25; Y10T436/25375
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
121 words
A method for continuously conditioning a cell suspension, comprising the following steps which occur simultaneously within a single container having a plurality of zones: introducing said cell suspension into first zone of said zones in said container; centrifuging the cell suspension in said first zone thereby separating a plurality of different components and at least one cellular component from said cell suspension and concentrating said cellular component; mixing the concentrated cellular component with a washing solution to form a washed cell suspension in a second zone of said zones in said container; and centrifuging said washed cell suspension in a third zones of said zones thereby separating said washing solution from a washed cellular component and concentrating said washed cellular component.
Claim 2
Independent Claim
121 words
A method for continuously conditioning a cell suspension, comprising the following steps which occur simultaneously within a single container having a plurality of zones: introducing said cell suspension into first zone of said zones in said container; centrifuging the cell suspension in said first zone thereby separating a plurality of different components and at least one cellular component from said cell suspension and concentrating said cellular component; mixing the concentrated cellular component with a washing solution to form a washed cell suspension in a second zone of said zones in said container; and centrifuging said washed cell suspension in a third zones of said zones thereby separating said washing solution from a washed cellular component and concentrating said washed cellular component.
Claim 3
Dependent Claim
12 words
The method as in claim 1, wherein the cell suspension comprises blood.
Claim 4
Dependent Claim
19 words
The method as in claim 2, further comprising a step of autotransfusing the washed cellular component to a patient.
Claim 5
Dependent Claim
12 words
The method as in claim 2, wherein said cellular component comprises erythrocytes.
Claim 6
Dependent Claim
27 words
The method as in claim 4, wherein the step of centrifuging said cell suspension is performed such that hematocrit readings of approximately 50 to 70% are achieved.
Claim 7
Dependent Claim
33 words
The method as in claim 4, wherein said step of centrifuging said washed cell suspension decreases said cellular component content to less than five percent of an initial quantity of said cell suspension.
Claim 8
Dependent Claim
24 words
The method as in claim 2, further comprising a step of providing one of isotonic sodium chloride and Ringer's solution as said washing solution.
Claim 9
Dependent Claim
18 words
The method as in claim 1, further comprising a step of removing said different components from said container.
Claim 10
Dependent Claim
19 words
The method as in claim 1 further comprising the step of removing said washed cellular component from said container.
Claim 11
Independent Claim
156 words
A method for separating and conditioning a cell suspension in a container having a plurality of inlets and outlets, said method comprising steps of: inputting said cell suspension in a first inlet of said inlets; centrifuging said cell suspension to separate a first cellular material and a second material from said cell suspension; outputting said second material from a first outlet of said outlets; inputting a wash material in a second inlet of said inlets; mixing said first cellular material with said wash material to form a washed suspension; centrifuging said washed suspension to separate a washed first cellular material from said washed suspension; outputting said washed suspension devoid of said washed first cellular material from a second outlet of said outlet, and outputting said washed first cellular material from said second outlet or a third outlet of said outlets, wherein said steps are performed simultaneously for respective portions of said cell suspension within said container.
Claim 12
Independent Claim
156 words
A method for separating and conditioning a cell suspension in a container having a plurality of inlets and outlets, said method comprising steps of: inputting said cell suspension in a first inlet of said inlets; centrifuging said cell suspension to separate a first cellular material and a second material from said cell suspension; outputting said second material from a first outlet of said outlets; inputting a wash material in a second inlet of said inlets; mixing said first cellular material with said wash material to form a washed suspension; centrifuging said washed suspension to separate a washed first cellular material from said washed suspension; outputting said washed suspension devoid of said washed first cellular material from a second outlet of said outlet, and outputting said washed first cellular material from said second outlet or a third outlet of said outlets, wherein said steps are performed simultaneously for respective portions of said cell suspension within said container.
Claim 13
Dependent Claim
18 words
The method as in claim 10, wherein said centrifuging steps separate said cell suspension based on differing densities.
Claim 14
Dependent Claim
24 words
The method as in claim 10, wherein said cell suspension comprises blood, said first cellular material comprises erythrocytes and said second material comprises plasma.
Claim 15
Dependent Claim
17 words
The method as in claim 12, further comprising a step of autotransfusing said erythrocytes to a patient.
Claim 16
Dependent Claim
25 words
The method as in claim 12, wherein a flow rate of said erythrocytes through said container is in a range of 60 to 100 ml/min.
Claim 17
Dependent Claim
19 words
The method as in claim 10, wherein said wash material comprises one of isotonic sodium chloride and Rinder's solution.
Claim 18
Dependent Claim
29 words
The method as in claim 10, wherein said step of centrifuging said washed suspension decreases said washed first cellular material to less than 5 percent of said cell suspension.
References Cited (Backward Citations)
DE2262856A1
Cited
US3825175A
Cited
US4014329A
Cited
US4010894A
Cited
US4934995A
Cited
US4386730A
Cited
US4216770A
Cited
EP0026334A1
Cited
US4447221A
Cited
EP0155684B1
Cited
US4647279A
Cited
US4886487A
Cited
US4927750A
Cited
US4804363A
Cited
WO1989001792A1
Cited
US5114396A
Cited
DE3817664C2
Cited
US5215519A
Cited
US5298171A
Cited
US5298016A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-03-03 | 2000-09-02 | 2001-03-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-03-03 | 2004-09-02 | 2005-03-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-03-03 | 2008-09-02 | 2009-03-04 | $7700.00 | Window Closed / Lapsed |