US5602301A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Non-human mammal having a graft and methods of delivering protein to myocardial tissue
Assignee: Indiana University Research and Technology Corp
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Filing: 1993-11-16
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Granted: 1997-02-11
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Expires: 2013-11-16
Abstract
Abstract Described are preferred myocardial grafts of skeletal myoblasts or cardiomyocytes, and cellular compositions and methods useful in obtaining the grafts. The myocardial grafts are stable and can be used, for example, to deliver recombinant proteins directly to the heart.
Inventors
- 👤 Loren J. Field
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 06:53
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0652; C12N5/0658; A; A01; A01K; A01K67/00; A01K67/027; A01K67/0271; A61; A61K; A61K35/00; A61K35/12; A61K35/34; A61K48/00; C07; C07K; C07K14/00; C07K14/435; C07K14/46; C07K14/47; C07K14/4701; C07K14/4716; C12N5/0657; A01K2217/00; A01K2217/05; C12N2500/00; C12N2500/05; C12N2500/10; C12N2500/20; C12N2500/22; C12N2506/00; C12N2506/02; C12N2510/00; C12N2517/00; C12N2517/02
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
29 words
A non-human mammal which has a myocardial graft, the mammal comprising: viable skeletal myoblasts or cardiomyocytes having a detectable marker directly introduced into the myocardial tissue of the mammal.
Claim 2
Dependent Claim
29 words
A non-human mammal which has a myocardial graft, the mammal comprising: viable skeletal myoblasts or cardiomyocytes having a detectable marker directly introduced into the myocardial tissue of the mammal.
Claim 3
Dependent Claim
10 words
The mammal of claim 1, wherein the graft is non-tumorigenic.
Claim 4
Dependent Claim
10 words
The mammal of claim 1, wherein the graft comprises cardiomyocytes.
Claim 5
Dependent Claim
11 words
The mammal of claim 1, wherein the graft comprises skeletal myoblasts.
Claim 6
Independent Claim
44 words
A method for forming a stable myocardial graft in a mammal, the method comprising: directly introducing viable skeletal myoblasts or cardiomyocytes into the myocardial tissue of the mammal such that the injected skeletal myoblasts or cardiomyocytes for a stable myocardial graft in the mammal.
Claim 7
Independent Claim
44 words
A method for forming a stable myocardial graft in a mammal, the method comprising: directly introducing viable skeletal myoblasts or cardiomyocytes into the myocardial tissue of the mammal such that the injected skeletal myoblasts or cardiomyocytes for a stable myocardial graft in the mammal.
Claim 8
Dependent Claim
18 words
The method of claim 5, wherein the introducing comprises injecting the myoblasts or cardiomyocytes into the myocardial tissue.
Claim 9
Dependent Claim
15 words
The method of claim 5, wherein skeletal myoblasts are directly introduced into the myocardial tissue.
Claim 10
Dependent Claim
14 words
The method of claim 5, wherein cardiomyocytes are directly introduced into the myocardial tissue.
Claim 11
Dependent Claim
11 words
The method of claim 5, wherein the myocardial graft is non-tumorigenic.
Claim 12
Independent Claim
64 words
A method for delivering proteins to the myocardial tissue of a mammal, the method comprising: directly introducing viable skeletal myoblasts or cardiomyocytes expressing a DNA sequence encoding a protein into the myocardial tissue of the mammal such that the injected skeletal myoblasts or cardiomyocytes form a stable graft, wherein the DNA sequence is expressed sufficiently for delivery of the protein to the myocardial tissue.
Claim 13
Independent Claim
64 words
A method for delivering proteins to the myocardial tissue of a mammal, the method comprising: directly introducing viable skeletal myoblasts or cardiomyocytes expressing a DNA sequence encoding a protein into the myocardial tissue of the mammal such that the injected skeletal myoblasts or cardiomyocytes form a stable graft, wherein the DNA sequence is expressed sufficiently for delivery of the protein to the myocardial tissue.
Claim 14
Dependent Claim
14 words
The method of claim 10, wherein the stable graft is comprised of skeletal myoblasts.
Claim 15
Dependent Claim
13 words
The method of claim 10, wherein the stable graft is comprised of cardiomyocytes.
Claim 16
Dependent Claim
11 words
The method of claim 10, wherein the stable graft is non-tumorigenic.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-02-11 | 2000-08-12 | 2001-02-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-02-11 | 2004-08-12 | 2005-02-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-02-11 | 2008-08-12 | 2009-02-11 | $7700.00 | Window Closed / Lapsed |