US5594138A
Expired / Lapsed
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Method of fluorescent detection of nucleic acids and cytoskeleton elements using bis-dicationic aryl furans
Assignee: University of North Carolina at Chapel Hill
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Filing: 1995-05-30
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Granted: 1997-01-14
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Expires: 2015-05-30
Abstract
Abstract Disclosed is a method of fluorescent detection of a nucleic acid. The method comprises contacting to the nucleic acid a bis-dicationic aryl furan compound, such as 2,5-bis[4-(4,5,6,7-tetrahydro-1H-1,3-diazepin-2-yl) phenyl] furan; 2,5-bis{[4-(N-isopropyl) amidino] phenyl}furan; and physiologically acceptable salts thereof, and exposing the nucleic acid to light at a frequency to induce fluorescence of the compound. A method for fluorescent detection of cytoskeleton elements, and novel bis-dicationic aryl furan compounds are also disclosed.
Inventors
- 👤 Christine C. Dykstra
- 👤 Richard R. Tidwell
- 👤 David W. Boykin
- 👤 W. David Wilson
- 👤 Jaroslaw Spychala
- 👤 Bijan P. Das
- 👤 Arvind Kumar
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 18:27
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; A; A61; A61K; A61K31/00; A61K31/33; A61K31/395; A61K31/495; A61K31/505; A61K31/506; C; C07; C07D; C07D307/00; C07D307/02; C07D307/34; C07D307/38; C07D307/54; C07D403/00; C07D403/02; C07D403/10; C07D403/14; C07D405/00; C07D405/14; C09; C09B; C09B23/00; C09B23/0075; Y; Y10; Y10S; Y10S435/00; Y10S435/808; Y10S435/81
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
58 words
A compound having the Formula (I) ##STR11## wherein: R 1 and R 2 together represent a C 2 to C 3 alkylene; R 3 is H; A is: ##STR12## wherein R 4 is H; and R 5 is OCH 3 or O(C 6 H 4 )R, wherein R is H or lower-alkyl; or a physiologically acceptable salt thereof.
Claim 2
Dependent Claim
58 words
A compound having the Formula (I) ##STR11## wherein: R 1 and R 2 together represent a C 2 to C 3 alkylene; R 3 is H; A is: ##STR12## wherein R 4 is H; and R 5 is OCH 3 or O(C 6 H 4 )R, wherein R is H or lower-alkyl; or a physiologically acceptable salt thereof.
Claim 3
Dependent Claim
51 words
A compound having the Formula (I) ##STR13## wherein: R 1 and R 2 together represent a C 2 linear, saturated alkylene; R 3 is --R 14 OH wherein R 14 is lower alkyl; A is: ##STR14## wherein R 4 and R 5 are each H; or a physiologically acceptable salt thereof.
Claim 4
Dependent Claim
51 words
A compound having the Formula (I) ##STR13## wherein: R 1 and R 2 together represent a C 2 linear, saturated alkylene; R 3 is --R 14 OH wherein R 14 is lower alkyl; A is: ##STR14## wherein R 4 and R 5 are each H; or a physiologically acceptable salt thereof.
Claim 5
Dependent Claim
15 words
A compound according to claim 2 wherein R 14 is --(CH 2 ) 2 --.
Claim 6
Dependent Claim
61 words
A compound having the Formula (I) ##STR15## wherein: R 1 and R 2 together represent a C 4 alkylene; R 3 is H, loweralkyl, cycloalkyl, aryl, hydroxyalkyl, aminoalkyl or alkylaminoalkyl; A is: ##STR16## wherein R 4 and R 5 are each independently selected from the group consisting of H, loweralkyl, halogen, oxyalkyl, oxyaryl, or oxyarylalkyl; or a physiologically acceptable salt thereof.
Claim 7
Dependent Claim
61 words
A compound having the Formula (I) ##STR15## wherein: R 1 and R 2 together represent a C 4 alkylene; R 3 is H, loweralkyl, cycloalkyl, aryl, hydroxyalkyl, aminoalkyl or alkylaminoalkyl; A is: ##STR16## wherein R 4 and R 5 are each independently selected from the group consisting of H, loweralkyl, halogen, oxyalkyl, oxyaryl, or oxyarylalkyl; or a physiologically acceptable salt thereof.
Claim 8
Dependent Claim
18 words
A compound according to claim 4, wherein R 3 , R 4 , and R 5 are H.
Claim 9
Dependent Claim
37 words
A compound having the Formula (I) ##STR17## wherein: R 1 and R 2 are H; R 3 is isopropyl; A is: ##STR18## wherein R 4 and R 5 are each H; or a physiologically acceptable salt thereof.
Claim 10
Dependent Claim
37 words
A compound having the Formula (I) ##STR17## wherein: R 1 and R 2 are H; R 3 is isopropyl; A is: ##STR18## wherein R 4 and R 5 are each H; or a physiologically acceptable salt thereof.
Claim 11
Dependent Claim
45 words
A compound having the Formula (I) ##STR19## wherein: R 1 and R 2 are H; R 3 is --(CH 2 ) 3 N(CH 3 ) 2 ; A is: ##STR20## wherein R 4 and R 5 are each H; or a physiologically acceptable salt thereof.
Claim 12
Dependent Claim
45 words
A compound having the Formula (I) ##STR19## wherein: R 1 and R 2 are H; R 3 is --(CH 2 ) 3 N(CH 3 ) 2 ; A is: ##STR20## wherein R 4 and R 5 are each H; or a physiologically acceptable salt thereof.
Claim 13
Dependent Claim
79 words
A compound having the Formula (I) ##STR21## wherein: R 1 and R 2 together are: ##STR22## wherein n is from 1 to 3 and R 10 is H or --CONHR 11 NR 15 R 16 where R 11 is loweralkyl and R 15 and R 16 are each independently selected from the group consisting of H or loweralkyl; R 3 is H; A is: ##STR23## wherein R 4 and R 5 are H; or a physiologically acceptable salt thereof.
Claim 14
Dependent Claim
79 words
A compound having the Formula (I) ##STR21## wherein: R 1 and R 2 together are: ##STR22## wherein n is from 1 to 3 and R 10 is H or --CONHR 11 NR 15 R 16 where R 11 is loweralkyl and R 15 and R 16 are each independently selected from the group consisting of H or loweralkyl; R 3 is H; A is: ##STR23## wherein R 4 and R 5 are H; or a physiologically acceptable salt thereof.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-01-14 | 2000-07-15 | 2001-01-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-01-14 | 2004-07-15 | 2005-01-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-01-14 | 2008-07-15 | 2009-01-14 | $7700.00 | Window Closed / Lapsed |