US5582697A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Biosensor, and a method and a device for quantifying a substrate in a sample liquid using the same
Assignee: PHC Holdings Corp
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Filing: 1995-04-20
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Granted: 1996-12-10
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Expires: 2015-04-20
Abstract
Abstract The biosensor of this invention can quantify a substrate in a sample liquid by electrochemically measuring the amount of an electron acceptor that has been reduced by electrons generated in a reaction between the substrate and an oxidoreductase. The biosensor has an electrically insulating substrate and an electrode system formed on the substrate including a working electrode, a counter electrode and a third electrode used for detecting a liquid junction. The third electrode can be used merely for detecting a liquid junction, or can be used as both a reference electrode and a liquid junction detecting electrode.
Inventors
- 👤 Shin Ikeda
- 👤 Toshihiko Yoshioka
- 👤 Shiro Nankai
- 👤 Haruhiro Tsutsumi
- 👤 Hideyuki Baba
- 👤 Yoshinobu Tokuno
- 👤 Syoji Miyazaki
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 20:07
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/005; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
133 words
A biosensor for quantifying a substrate in a sample liquid by electrochemically measuring an amount of an electron acceptor that has been reduced by electrons generated in a reaction between the substrate and an oxidoreductase, comprising: an electrically insulating substrate; an electrode system formed on the substrate including a working electrode, a counter electrode and a third electrode used for detecting a liquid junction; and a reaction layer that is formed over at least the working electrode and the counter electrode of the electrode system and includes the oxidoreductase, wherein the third electrode is disposed farther from a sample supply port than the working electrode and the counter electrode, so that a sample liquid supplied through the sample supply port reaches the third electrode after reaching the working electrode and the counter electrode.
Claim 2
Dependent Claim
133 words
A biosensor for quantifying a substrate in a sample liquid by electrochemically measuring an amount of an electron acceptor that has been reduced by electrons generated in a reaction between the substrate and an oxidoreductase, comprising: an electrically insulating substrate; an electrode system formed on the substrate including a working electrode, a counter electrode and a third electrode used for detecting a liquid junction; and a reaction layer that is formed over at least the working electrode and the counter electrode of the electrode system and includes the oxidoreductase, wherein the third electrode is disposed farther from a sample supply port than the working electrode and the counter electrode, so that a sample liquid supplied through the sample supply port reaches the third electrode after reaching the working electrode and the counter electrode.
Claim 3
Dependent Claim
72 words
A biosensor according to claim 1, wherein the counter electrode includes a main electrode portion formed in substantially a C-shape in a plane view and an opening formed in the main electrode portion, the working electrode disposed inside of the counter electrode to be electrically insulated from each other, and a lead connected to the working electrode is led from the inside to the outside of the counter electrode through the opening.
Claim 4
Dependent Claim
17 words
A biosensor according to claim 1, wherein the reaction layer is formed also over the third electrode.
Claim 5
Dependent Claim
31 words
A biosensor according to claim 1, wherein a cover is provided over the substrate, and a space serving as a sample supply path is formed between the substrate and the cover.
Claim 6
Dependent Claim
15 words
A biosensor according to claim 4, wherein the electrode system is exposed to the space.
Claim 7
Dependent Claim
15 words
A biosensor according to claim 1, wherein the reaction layer further includes an electron acceptor.
Claim 8
Dependent Claim
15 words
A biosensor according to claim 1, wherein the reaction layer further includes a hydrophilic polymer.
Claim 9
Dependent Claim
102 words
A method for quantifying a substrate in a sample liquid by using the biosensor according to claim 1, comprising the steps of: applying a voltage between the counter electrode and the third electrode; supplying a sample liquid to the reaction layer; detecting an electrical change between the counter electrode and the third electrode caused by supplying the sample liquid to the reaction layer; applying a voltage between the working electrode and both of the third electrode and the counter electrode after detecting the electrical change; and measuring a current flowing between the counter electrode and the working electrode after applying the voltage.
Claim 10
Dependent Claim
96 words
A method for quantifying a substrate in a sample liquid by using the biosensor according to claim 1, comprising the steps of: applying a voltage between the counter electrode and the third electrode; supplying a sample liquid to the reaction layer; detecting an electrical change between the counter electrode and the third electrode caused by supplying the sample liquid to the reaction layer; applying a voltage between the working electrode and the counter electrode after detecting the electrical change; and measuring a current flowing between the counter electrode and the working electrode after applying the voltage.
Claim 11
Dependent Claim
81 words
A quantifying device for a substrate contained in a sample liquid comprising: the biosensor according to claim 1; means for detecting an electrical change between the counter electrode and the third electrode caused by supplying a sample liquid to the reaction layer; means for applying a voltage between the working electrode and both of the third electrode and the counter electrode after detecting the electrical change; and means for measuring a current flowing between the working electrode and the counter electrode.
Claim 12
Dependent Claim
75 words
A quantifying device for a substrate contained in a sample liquid comprising: the biosensor according to claim 1; means for detecting an electrical change between the counter electrode and the third electrode caused by supplying a sample liquid to the reaction layer; means for applying a voltage between the working electrode and the counter electrode after detecting the electrical change; and means for measuring a current flowing between the working electrode and the counter electrode.
Claim 13
Dependent Claim
198 words
A quantifying device for a substrate contained in a sample liquid, comprising the biosensor according to claim 1 and a measuring device to which the biosensor is removably connected, wherein the measuring device includes: current/voltage converting circuits connected to the third electrode of the biosensor; A/D converting circuits connected to the current/voltage converting circuits; current/voltage converting circuits capable of being connected to the working electrode of the biosensor via a switch; A/D converting circuits connected to the current/voltage converting circuits; and a controller connected to the respective A/D converting circuits, the switch being turned ON or OFF with respect to the working electrode by a control of the controller, and wherein an electrical change between the counter electrode and the third electrode, caused by a supply of a sample liquid to the reactive layer, is detected by the control portion under a condition that the switch is insulated from the working electrode, and then a voltage is applied between the working electrode and both of the third electrode and the counter electrode under a condition that the switch is connected to the working electrode and a current flowing between the working electrode and the counter electrode is measured.
Claim 14
Dependent Claim
192 words
A quantifying device for a substrate contained in a sample liquid, comprising the biosensor according to claim 1 and a measuring device to which the biosensor is removably connected, wherein the measuring device includes: current/voltage converting circuits connected to the third electrode of the biosensor; A/D converting circuits connected to the current/voltage converting circuits; current/voltage converting circuits capable of being connected to the working electrode of the biosensor via a switch; A/D converting circuits connected to the current/voltage converting circuits; and a controller connected to the respective A/D converting circuits, the switch being turned ON or OFF with respect to the working electrode by a control of the controller, and wherein an electrical change between the counter electrode and the third electrode, caused by a supply of a sample liquid to the reactive layer, is detected by the control portion under a condition that the switch is insulated from the working electrode, and then a voltage is applied between the working electrode and the counter electrode under a condition that the switch is connected to the working electrode and a current flowing between the working electrode and the counter electrode is measured.
Claim 15
Dependent Claim
170 words
A quantifying device for a substrate contained in a sample liquid, comprising the biosensor according to claim 1 and a measuring device to which the biosensor is removably connected, wherein the measuring device includes: current/voltage converting circuits switchably connected to the third electrode of the biosensor and the working electrode; A/D converting circuits connected to the current/voltage converting circuits; a controller connected to the respective A/D converting circuits, the switch being alternatively connected to the working electrode and the third electrode by a control of the controller, and wherein an electrical change between the counter electrode and the third electrode, caused by a supply of a sample liquid to the reactive layer, is detected by the control portion under a condition that the switch is connected to the third electrode, and then a voltage is applied between the working electrode and the counter electrode under a condition that the switch is connected to the working electrode and a current flowing between the working electrode and the counter electrode is measured.
Claim 16
Dependent Claim
116 words
A biosensor for quantifying a substrate in a sample liquid by electrochemically measuring an amount of an electron acceptor that has been reduced by electrons generated in a reaction between the substrate and an oxidoreductase, comprising: an electrically insulating substrate; an electrode system formed on the substrate including a working electrode, a counter electrode and a third electrode used for detecting a liquid junction; and a reaction layer that is formed over at least the working electrode and the counter electrode of the electrode system and includes the oxidoreductase, wherein a peripheral portion of the counter electrode is opened to provide an electrode receiving portion, and the third electrode is disposed within the electrode receiving portion.
Claim 17
Dependent Claim
116 words
A biosensor for quantifying a substrate in a sample liquid by electrochemically measuring an amount of an electron acceptor that has been reduced by electrons generated in a reaction between the substrate and an oxidoreductase, comprising: an electrically insulating substrate; an electrode system formed on the substrate including a working electrode, a counter electrode and a third electrode used for detecting a liquid junction; and a reaction layer that is formed over at least the working electrode and the counter electrode of the electrode system and includes the oxidoreductase, wherein a peripheral portion of the counter electrode is opened to provide an electrode receiving portion, and the third electrode is disposed within the electrode receiving portion.
Claim 18
Dependent Claim
175 words
A biosensor for quantifying a substrate in a sample liquid by electrochemically measuring an amount of an electron acceptor that has been reduced by electrons generated in a reaction between the substrate and an oxidoreductase, comprising: an electrically insulating substrate; an electrode system formed on the substrate including a working electrode, a counter electrode and a third electrode used for detecting a liquid junction; and a reaction layer that is formed over at least the working electrode and the counter electrode of the electrode system and includes the oxidoreductase, wherein the counter electrode includes a main electrode portion formed in substantially a C-shape in a plane view and an opening formed in the main electrode portion, the working electrode disposed inside of the counter electrode to be electrically insulated from each other, a lead connected to the working electrode is led from the inside to the outside of the counter electrode through the opening, and the third electrode is disposed between the counter electrode and the working electrode to be electrically insulated from one another.
Claim 19
Dependent Claim
175 words
A biosensor for quantifying a substrate in a sample liquid by electrochemically measuring an amount of an electron acceptor that has been reduced by electrons generated in a reaction between the substrate and an oxidoreductase, comprising: an electrically insulating substrate; an electrode system formed on the substrate including a working electrode, a counter electrode and a third electrode used for detecting a liquid junction; and a reaction layer that is formed over at least the working electrode and the counter electrode of the electrode system and includes the oxidoreductase, wherein the counter electrode includes a main electrode portion formed in substantially a C-shape in a plane view and an opening formed in the main electrode portion, the working electrode disposed inside of the counter electrode to be electrically insulated from each other, a lead connected to the working electrode is led from the inside to the outside of the counter electrode through the opening, and the third electrode is disposed between the counter electrode and the working electrode to be electrically insulated from one another.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-12-10 | 2000-06-10 | 2000-12-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-12-10 | 2004-06-10 | 2004-12-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-12-10 | 2008-06-10 | 2008-12-10 | $7700.00 | Window Closed / Lapsed |