US5569595A
Expired / Lapsed
US
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Production of poly-β-hydroxybutyrate in prokaryotic host cells
Assignee: James Madison University
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Filing: 1993-03-24
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Granted: 1996-10-29
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Expires: 2013-03-24
Abstract
Abstract The present invention provides methods for the production of poly- beta -hydroxybutyrate, comprising the steps of (a) introducing into a prokaryotic host cell capable of metabolizing sucrose a vector construct which directs the expression of a sequence which encodes a poly- beta -hydroxybutyrate biosynthetic pathway, (b) culturing the host cell in medium containing sucrose, and (c) isolating poly- beta -hydroxybutyrate from the cultured host cell.
Inventors
- 👤 Douglas E. Dennis
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 22:56
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/195; C07K14/225; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/52; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2462; C12N9/93; C12P; C12P7/00; C12P7/62; C12P7/625
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
67 words
A method for the production of poly-β-hydroxybutyrate, comprising: (a) introducing into a prokanyotic host cell capable of metabolizing sucrose a vector construct which directs the expression of a sequence which encodes a poly-β-hydroxybutyrate biosynthetic pathway, said prokaryotic host cell selected from the group consisting of E. coli and Klebsiella; (b) culturing said host cell in medium containing sucrose; and (c) isolating poly-β-hydroxybutyrate from said cultured host cell.
Claim 2
Independent Claim
67 words
A method for the production of poly-β-hydroxybutyrate, comprising: (a) introducing into a prokanyotic host cell capable of metabolizing sucrose a vector construct which directs the expression of a sequence which encodes a poly-β-hydroxybutyrate biosynthetic pathway, said prokaryotic host cell selected from the group consisting of E. coli and Klebsiella; (b) culturing said host cell in medium containing sucrose; and (c) isolating poly-β-hydroxybutyrate from said cultured host cell.
Claim 3
Independent Claim
82 words
A method for the production of poly-β-hydroxyalkanoate copolymer, comprising: (a) introducing into a prokaryotic host cell capable of metabolizing sucrose and expressing acetate utilization enzymes constitutively, a vector construct which directs the expression of a sequence which encodes a poly-β-hydroxybutyrate biosynthetic pathway, said prokaryotic host cell selected from the group consisting of E. coli and Klebsiella; (b) culturing said host cell in medium containing propionate or a derivative thereof, and sucrose; and (c) isolating said poly-β-hydroxyalkanoate copolymer from said cultured host cell.
Claim 4
Independent Claim
82 words
A method for the production of poly-β-hydroxyalkanoate copolymer, comprising: (a) introducing into a prokaryotic host cell capable of metabolizing sucrose and expressing acetate utilization enzymes constitutively, a vector construct which directs the expression of a sequence which encodes a poly-β-hydroxybutyrate biosynthetic pathway, said prokaryotic host cell selected from the group consisting of E. coli and Klebsiella; (b) culturing said host cell in medium containing propionate or a derivative thereof, and sucrose; and (c) isolating said poly-β-hydroxyalkanoate copolymer from said cultured host cell.
Claim 5
Dependent Claim
14 words
The method of claim 1 or 2 wherein said host cell is E. coli.
Claim 6
Dependent Claim
13 words
The method of claim 1 or 2 wherein said host cell is Klebsiella.
Claim 7
Dependent Claim
12 words
The method of claim 4 wherein said host cell is Klebsiella aerogenes.
Claim 8
Independent Claim
17 words
The method of claims 1 or 2 wherein said medium contains sucrose as the principal carbon source.
Claim 9
Independent Claim
12 words
The method of claims 1 or 2 wherein said medium comprises molasses.
Claim 10
Dependent Claim
25 words
E. coli capable of metabolizing sucrose and containing a vector construct which directs the expression of a sequence which encodes a biosynthetic pathway of poly-β-hydroxybutyrate.
Claim 11
Dependent Claim
25 words
E. coli capable of metabolizing sucrose and containing a vector construct which directs the expression of a sequence which encodes a biosynthetic pathway of poly-β-hydroxybutyrate.
Claim 12
Dependent Claim
24 words
Klebsiella capable of metabolizing sucrose and containing a vector construct which directs the expression of a sequence which encodes a biosynthetic pathway of poly-β-hydroxybutyrate.
Claim 13
Dependent Claim
24 words
Klebsiella capable of metabolizing sucrose and containing a vector construct which directs the expression of a sequence which encodes a biosynthetic pathway of poly-β-hydroxybutyrate.
Claim 14
Independent Claim
53 words
A method for the production of poly-β-hydroxybutyrate, comprising: (a) introducing into a Klebsiella host cell capable of metabolizing sucrose, a vector construct which directs the expression of a sequence which encodes a poly-β-hydroxybutyrate biosynthetic pathway; (b) culturing said host cell in medium containing sucrose; and (c) isolating poly-β-hydroxybutyrate from said cultured host cell.
Claim 15
Independent Claim
53 words
A method for the production of poly-β-hydroxybutyrate, comprising: (a) introducing into a Klebsiella host cell capable of metabolizing sucrose, a vector construct which directs the expression of a sequence which encodes a poly-β-hydroxybutyrate biosynthetic pathway; (b) culturing said host cell in medium containing sucrose; and (c) isolating poly-β-hydroxybutyrate from said cultured host cell.
Claim 16
Independent Claim
68 words
A method for the production of poly-β-hydroxyalkanoate copolymer, comprising: (a) introducing into a Klebsiella host cell capable of metabolizing sucrose and expressing acetate utilization enzymes constitutively, a vector construct which directs the expression of a sequence which encodes a poly-β-hydroxybutyrate biosynthetic pathway; (b) culturing said host cell in medium containing propionate or a derivative thereof, and sucrose; and (c) isolating said poly-β-hydroxyalkanoate copolymer from said cultured host cell.
Claim 17
Independent Claim
68 words
A method for the production of poly-β-hydroxyalkanoate copolymer, comprising: (a) introducing into a Klebsiella host cell capable of metabolizing sucrose and expressing acetate utilization enzymes constitutively, a vector construct which directs the expression of a sequence which encodes a poly-β-hydroxybutyrate biosynthetic pathway; (b) culturing said host cell in medium containing propionate or a derivative thereof, and sucrose; and (c) isolating said poly-β-hydroxyalkanoate copolymer from said cultured host cell.
Claim 18
Dependent Claim
12 words
The method of claim 4 wherein said host cell is Klebsiella oxytoca.
Claim 19
Dependent Claim
14 words
The method of claim 10 or 11 wherein said host cell is Klebsiella aerogenes.
Claim 20
Dependent Claim
14 words
The method of claim 10 or 11 wherein said host cell is Klebsiella oxytoca.
Claim 21
Dependent Claim
12 words
The method of claim 4 wherein said host cell is Klebsiella oxytoca.
Claim 22
Dependent Claim
11 words
The Klebsiella of claim 9 wherein said Klebsiella is Klebsiella aerogenes.
Claim 23
Dependent Claim
11 words
The Klebsiella of claim 9 wherein said Klebsiella is Klebsiella oxytoca.
References Cited (Backward Citations)
US4806480A
Cited
US5245023A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-10-29 | 2000-04-29 | 2000-10-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-10-29 | 2004-04-29 | 2004-10-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-10-29 | 2008-04-29 | 2008-10-29 | $7700.00 | Window Closed / Lapsed |