US5565085A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for quantifying specific compound
Assignee: PHC Holdings Corp
•
Filing: 1995-04-25
•
Granted: 1996-10-15
•
Expires: 2015-04-25
Abstract
Abstract A biosensor for quantifying a specific compound has a reaction layer containing at least an enzyme, and an electrode system having a working electrode and a counter electrode formed on an insulating base plate; the biosensor detects the specific compound contained in a sample on the basis of an electrochemical response. In quantifying, the working electrode and the counter electrode are short-circuited before the voltage is applied therebetween. The short-circuiting eliminates measuring errors that may occur due to nonuniform dissolution of the reaction layer in a sample, and achieves highly reliable quantification of a specific compound.
Inventors
- 👤 Shin Ikeda
- 👤 Toshihiko Yoshioka
- 👤 Shiro Nankai
- 👤 Haruhiro Tsutsumi
- 👤 Hideyuki Baba
- 👤 Yoshinobu Tokuno
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 17:57
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
140 words
A method for quantifying a specific compound, which uses a biosensor comprising a reaction layer containing at least an enzyme and an electron acceptor, and an electrode system having a working electrode made of carbon and a counter electrode made of carbon formed on an insulating base plate, and which detects a change in substance concentration caused by a reaction between said enzyme and a specific compound contained in a sample by detecting an electrochemical response obtained when a voltage is applied between said working electrode and said counter electrode, wherein said electrochemical response is an oxidation current of a reduced form of said electron acceptor, and said working electrode and said counter electrode are short-circuited by closing a switch in a circuit connected to terminals of said working electrode and said counter electrode before said voltage is applied therebetween.
Claim 2
Independent Claim
140 words
A method for quantifying a specific compound, which uses a biosensor comprising a reaction layer containing at least an enzyme and an electron acceptor, and an electrode system having a working electrode made of carbon and a counter electrode made of carbon formed on an insulating base plate, and which detects a change in substance concentration caused by a reaction between said enzyme and a specific compound contained in a sample by detecting an electrochemical response obtained when a voltage is applied between said working electrode and said counter electrode, wherein said electrochemical response is an oxidation current of a reduced form of said electron acceptor, and said working electrode and said counter electrode are short-circuited by closing a switch in a circuit connected to terminals of said working electrode and said counter electrode before said voltage is applied therebetween.
Claim 3
Dependent Claim
43 words
A method for quantifying a specific compound in accordance with claim 1, wherein alternation of a short circuit state and an open circuit state is repeated a plurality of times before said voltage is applied between said working electrode and said counter electrode.
Claim 4
Independent Claim
153 words
An apparatus for quantifying a specific compound, comprising: a biosensor including a reaction layer containing at least an enzyme and an electron acceptor, and an electrode system having a working electrode made of carbon and a counter electrode made of carbon formed on an insulating base plate; means for supplying a sample solution containing a specific compound to said reaction layer of said biosensor; voltage application means for applying a predetermined voltage between said working electrode and said counter electrode of the biosensor; means for measuring an oxidation current of a reduced form of said electron acceptor flowing between said working electrode and said counter electrode to which said voltage is applied; means for short-circuiting said working electrode and said counter electrode by closing a switch in a circuit connected to terminals of said working electrode and said counter electrode before said voltage is applied between said working electrode and said counter electrode.
Claim 5
Independent Claim
153 words
An apparatus for quantifying a specific compound, comprising: a biosensor including a reaction layer containing at least an enzyme and an electron acceptor, and an electrode system having a working electrode made of carbon and a counter electrode made of carbon formed on an insulating base plate; means for supplying a sample solution containing a specific compound to said reaction layer of said biosensor; voltage application means for applying a predetermined voltage between said working electrode and said counter electrode of the biosensor; means for measuring an oxidation current of a reduced form of said electron acceptor flowing between said working electrode and said counter electrode to which said voltage is applied; means for short-circuiting said working electrode and said counter electrode by closing a switch in a circuit connected to terminals of said working electrode and said counter electrode before said voltage is applied between said working electrode and said counter electrode.
Claim 6
Dependent Claim
34 words
An apparatus for quantifying a specific compound in accordance with claim 3, further comprising means for alternately closing and opening a circuit between said working electrode and said counter electrode a plurality of times.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-10-15 | 2000-04-15 | 2000-10-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-10-15 | 2004-04-15 | 2004-10-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-10-15 | 2008-04-15 | 2008-10-15 | $7700.00 | Window Closed / Lapsed |