US5561046A
Expired / Lapsed
US
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Methods for the detection of acid-labile subunit (ALS) of insulin-like growth factor binding protein complex
Assignee: Sydney South West Area Health Service
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Filing: 1994-03-14
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Granted: 1996-10-01
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Expires: 2014-03-14
Abstract
Abstract An acid-labile protein is described that, when incubated with the 53-Kd acid-stable protein occupied by IGF, converts it to a high molecular weight complex, corresponding to the in vivo form of IGF. This protein is called the acid-labile subunit (ALS) of IGF binding protein complex and is provided in biologically pure form. ALS is useful in treating wounds and promoting cellular growth. The nucleic acid encoding ALS, antibodies binding thereto, and fragments thereof are also disclosed.
Inventors
- 👤 Robert C. Baxter
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 03:03
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; C07K16/26; C07K14/00; C07K14/435; C07K14/46; C07K14/47; C07K14/4701; C07K14/4743; A; A61; A61K; A61K38/00; Y; Y10; Y10T; Y10T436/00; Y10T436/25; Y10T436/25375; Y10T436/255
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
60 words
A method for quantitating free acid labile-subunit (ALS) and complexed ALS (ALS-BP-53-IGF) in a body fluid, which comprises: (a) dialyzing said body fluid; (b) fractionating the body fluid formed in step (a) on a fractionation matrix to obtain free ALS separate from complexed ALS (ALS-BP-53-IGF); and c) quantitating the free ALS and the complexed ALS (ALS-BP-53-IGF) obtained in step (b).
Claim 2
Independent Claim
60 words
A method for quantitating free acid labile-subunit (ALS) and complexed ALS (ALS-BP-53-IGF) in a body fluid, which comprises: (a) dialyzing said body fluid; (b) fractionating the body fluid formed in step (a) on a fractionation matrix to obtain free ALS separate from complexed ALS (ALS-BP-53-IGF); and c) quantitating the free ALS and the complexed ALS (ALS-BP-53-IGF) obtained in step (b).
Claim 3
Dependent Claim
15 words
The method of claim 1, wherein the free and complexed ALS are quantitated by immunoassay.
Claim 4
Independent Claim
55 words
A method for detecting acid-labile subunit (ALS) in a biological fluid comprising the steps of: (a) binding proteins of a biological fluid to a support matrix; (b) reacting the support matrix to which the proteins are bound with an antibody which specifically binds ALS to form an ALS-antibody complex; and (c) detecting said ALS-antibody complex.
Claim 5
Independent Claim
55 words
A method for detecting acid-labile subunit (ALS) in a biological fluid comprising the steps of: (a) binding proteins of a biological fluid to a support matrix; (b) reacting the support matrix to which the proteins are bound with an antibody which specifically binds ALS to form an ALS-antibody complex; and (c) detecting said ALS-antibody complex.
Claim 6
Independent Claim
94 words
A method of quantitating free acid labile-subunit (ALS) in a sample of body fluid containing both free ALS and complexed ALS (ALS-BP-53-IGF), which comprises: a) contacting the sample with a trace-labelled BP-53-IGF complex to form an ALS-BP-53-IGF complex; b) fractionating the ALS-BP-53-IGF complex formed in step a) on a size fractionation matrix to separate said BP-53-IGF complex from said ALS-BP-53-IGF complex; c) measuring the label present in said BP-53-IGF complex and said ALS-BP-53-IGF complex; and d) determining the ratio of said ALS-BP-53-IGF complex to said BP-53-IGF complex as a quantitative index of free ALS.
Claim 7
Independent Claim
94 words
A method of quantitating free acid labile-subunit (ALS) in a sample of body fluid containing both free ALS and complexed ALS (ALS-BP-53-IGF), which comprises: a) contacting the sample with a trace-labelled BP-53-IGF complex to form an ALS-BP-53-IGF complex; b) fractionating the ALS-BP-53-IGF complex formed in step a) on a size fractionation matrix to separate said BP-53-IGF complex from said ALS-BP-53-IGF complex; c) measuring the label present in said BP-53-IGF complex and said ALS-BP-53-IGF complex; and d) determining the ratio of said ALS-BP-53-IGF complex to said BP-53-IGF complex as a quantitative index of free ALS.
Claim 8
Dependent Claim
9 words
A polyclonal antibody which specifically binds acid-labile subunit (ALS).
Claim 9
Dependent Claim
9 words
A polyclonal antibody which specifically binds acid-labile subunit (ALS).
Claim 10
Dependent Claim
9 words
A monoclonal antibody which specifically binds acid-labile subunit (ALS).
Claim 11
Dependent Claim
9 words
A monoclonal antibody which specifically binds acid-labile subunit (ALS).
Claim 12
Dependent Claim
15 words
The antibody according to claim 5 or 6 which is labelled with a reporter group.
Claim 13
Dependent Claim
17 words
The antibody of claim 7, wherein the reporter group is a fluorescent group, enzyme or colloidal group.
References Cited (Backward Citations)
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