Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
210 words
A particle counting method comprising: passing plural kinds of particles substantially one at a time through a narrow path; measuring values of at least two kinds of characteristics of each particle in said narrow path; preparing a scattergram of at least two dimensions using said values as parameters thereof; partitioning said particles in said scattergram into clusters of said plural kinds; defining a fixed domain for each cluster of said scattergram so that all of the particles within said domain belong to said cluster without exception and assigning a membership value of one to all particles within said domain; calculating an initial center of gravity of each cluster based upon the spatial distribution of the particles throughout said cluster; calculating a distance from said particle distributed outside each said fixed domain in said scattergram to the initial center of gravity of said cluster and calculating the membership value of said particle to each cluster based upon said distance; correcting said initial center of gravity of each cluster in consideration of the value of membership of each particle distributed outside said fixed domains to each cluster; calculating an estimated number of particles belonging to each cluster based upon the value of membership of each particle in said scattergram to each cluster.
Claim 2
Dependent Claim
210 words
A particle counting method comprising: passing plural kinds of particles substantially one at a time through a narrow path; measuring values of at least two kinds of characteristics of each particle in said narrow path; preparing a scattergram of at least two dimensions using said values as parameters thereof; partitioning said particles in said scattergram into clusters of said plural kinds; defining a fixed domain for each cluster of said scattergram so that all of the particles within said domain belong to said cluster without exception and assigning a membership value of one to all particles within said domain; calculating an initial center of gravity of each cluster based upon the spatial distribution of the particles throughout said cluster; calculating a distance from said particle distributed outside each said fixed domain in said scattergram to the initial center of gravity of said cluster and calculating the membership value of said particle to each cluster based upon said distance; correcting said initial center of gravity of each cluster in consideration of the value of membership of each particle distributed outside said fixed domains to each cluster; calculating an estimated number of particles belonging to each cluster based upon the value of membership of each particle in said scattergram to each cluster.
Claim 3
Dependent Claim
64 words
A method as set forth in claim 1, characterized in that said distance is calculated as a half length of the minor axis of an ellipse having its center at said initial center of gravity of each cluster and a predetermined ratio of the major and minor axes and tilt angle of the major axis which are predetermined for each cluster passing said particle.
Claim 4
Dependent Claim
37 words
A method as set forth in claim 1, characterized in that said number of particles is calculated as assuming that each particle belongs only to the cluster which is greatest in the calculated value of membership thereof.
Claim 5
Dependent Claim
39 words
A method as set forth in claim 1, characterized in that, said number of particles is calculated as assuming that each particle is allotted to each cluster in proportion to the calculated value of membership thereof to said cluster.
Claim 6
Dependent Claim
38 words
A method as set forth in claim 1, further comprising the step of repeating said membership value calculating step and center of gravity calculating step until a value of correction of said center of gravity becomes sufficiently small.
Claim 7
Dependent Claim
22 words
A method as set forth in claim 1, wherein said sample is blood and said particles are different types of blood cells.
Claim 8
Dependent Claim
24 words
A method as set forth in claim 1, further comprising the step of applying direct current and high frequency current to said narrow path.