US5543301A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of identifying Bacillus cereus having biocontrol activity
Assignee: Wisconsin Alumni Research Foundation
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Filing: 1994-03-30
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Granted: 1996-08-06
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Expires: 2014-03-30
Abstract
Abstract The invention includes a substantially pure preparation of Bacillus cereus antibiotic. The invention further includes a seed inoculum for application to seeds to be protected from damping off and root rot including a non-interfering carrier and an effective quantity of Bacillus cereus antibiotic, and a method for protecting plants in a growing medium from damping off and root rot including the step of placing in the growing medium in the immediate vicinity of the plant to be protected an effective quantity of Bacillus cereus antibiotic.
Inventors
- 👤 Jo Handelsman
- 👤 Larry Halverson
- 👤 Eric V. Stabb
- 👤 Benjamin Lethbridge
- 👤 Laura Silo
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 02:45
CPC Classifications
C; C12; C12P; C12P1/00; C12P1/04; A; A01; A01N; A01N63/00; A01N63/20; A01N63/22; C12N; C12N1/00; C12N1/20; C12N1/205; C12R; C12R2001/00; C12R2001/01; C12R2001/07; C12R2001/085
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
79 words
A method of identifying Bacillus cereus strains having biocontrol activity comprising the following steps in order: (a) isolating strains of bacteria from soil or roots of legumes; (b) selecting for strains having all of the taxonomic characteristics of Bacillus cereus; (c) selecting the Bacillus cereus strains obtained in (b) that are susceptible to phage P7, as demonstrated by lysis thereof; and (d) verifying biocontrol activity by identifying the cells obtained in (c) which inhibit the growth of Erwinia herbicola.
Claim 2
Independent Claim
79 words
A method of identifying Bacillus cereus strains having biocontrol activity comprising the following steps in order: (a) isolating strains of bacteria from soil or roots of legumes; (b) selecting for strains having all of the taxonomic characteristics of Bacillus cereus; (c) selecting the Bacillus cereus strains obtained in (b) that are susceptible to phage P7, as demonstrated by lysis thereof; and (d) verifying biocontrol activity by identifying the cells obtained in (c) which inhibit the growth of Erwinia herbicola.
Claim 3
Dependent Claim
27 words
The method of claim 1 further comprising the following step: (e) identifying the Bacillus cereus strains obtained in (d) which produce the antibiotic zwittermicin by assaying therefor.
Claim 4
Independent Claim
78 words
A method of identifying Bacillus cereus strains producing zwittermicin antibiotic comprising the following steps in order: (a) isolating strains of bacteria from soil or roots of legumes; (b) selecting for strains having all of the taxonomic characteristics of Bacillus cereus; (c) selecting the Bacillus cereus strains obtained in (b) that are susceptible to phage P7, as demonstrated by lysis thereof; and (d) selecting the Bacillus cereus strains obtained in (c) which produce the antibiotic zwittermicin by assaying therefor.
Claim 5
Independent Claim
78 words
A method of identifying Bacillus cereus strains producing zwittermicin antibiotic comprising the following steps in order: (a) isolating strains of bacteria from soil or roots of legumes; (b) selecting for strains having all of the taxonomic characteristics of Bacillus cereus; (c) selecting the Bacillus cereus strains obtained in (b) that are susceptible to phage P7, as demonstrated by lysis thereof; and (d) selecting the Bacillus cereus strains obtained in (c) which produce the antibiotic zwittermicin by assaying therefor.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-08-06 | 2000-02-05 | 2000-08-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-08-06 | 2004-02-05 | 2004-08-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-08-06 | 2008-02-05 | 2008-08-06 | $7700.00 | Window Closed / Lapsed |