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US5539084A
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Method for the use and synthesis of peptides
Assignee: Mimotopes Pty Ltd
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Filing: 1990-02-16
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Granted: 1996-07-23
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Expires: 2010-02-16
Abstract
Abstract PCT No. PCT/AU90/00062 Sec. 371 Date Sep. 6, 1991 Sec. 102(e) Date Sep. 6, 1991 PCT Filed Feb. 16, 1990 PCT Pub. No. WO90/09395 PCT Pub. Date Aug. 23, 1990A method for the separation of at least one specific binding entity from a mixture of binding entities, which comprises the steps of contacting said mixture of binding entities with an immobilized peptide in which said peptide specifically binds to said specific binding entity, and separating the immoblized peptide/specific binding entity complex from the mixture of binding entities; an immobilized peptide-solid support preparation comprising a cleavable link between the peptide and the solid support, and a method for the preparation of a peptide from an immobilized peptide-solid support preparation.
Inventors
- 👤 Hendrik M. Geysen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:51
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/195; C07K14/35; C07K1/00; C07K1/04; C07K1/047; C07K1/14; C07K1/16; C07K1/22; Y; Y10; Y10S; Y10S436/00; Y10S436/824; Y10S530/00; Y10S530/81
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
50 words
A compound of the formula: ##STR1## wherein (X) is a solid-phase peptide synthesis stable protecting group; R is an amino acid side chain having a functional group; and one of P 1 and P 2 is a peptide, and the other is a solid support with or without a spacer.
Claim 2
Dependent Claim
50 words
A compound of the formula: ##STR1## wherein (X) is a solid-phase peptide synthesis stable protecting group; R is an amino acid side chain having a functional group; and one of P 1 and P 2 is a peptide, and the other is a solid support with or without a spacer.
Claim 3
Dependent Claim
12 words
The compound of claim 1, wherein P 2 is a solid support.
Claim 4
Dependent Claim
10 words
The compound of claim 2, wherein said formula is: ##STR2##
Claim 5
Dependent Claim
10 words
The compound of claim 1, wherein said formula is: ##STR3##
Claim 6
Dependent Claim
14 words
The compound of claim 1, wherein said spacer is CH 2 CH 2 NH.
Claim 7
Dependent Claim
6 words
A compound of the formula: ##STR4##
Claim 8
Dependent Claim
6 words
A compound of the formula: ##STR4##
Claim 9
Independent Claim
117 words
A method for preparing a derivatized peptide, said method comprising: (i) providing a support of the formula: ##STR5## wherein (X) is a solid-phase peptide synthesis stable protecting group, and n is 0 or 1; (ii) coupling a protected amino acid to the free NH 2 to provide a protected oligopeptide; (iii) deprotecting the protected oligopeptide to provide a new free NH 2 ; (iv) coupling a protected amino acid to the new free NH 2 to provide a new protected oligopeptide; (v) repeating steps (iii) and (iv) until the desired peptide sequence is obtained; (vi) removing (X) to provide a deprotected amine; and (vii) treating said deprotected amine with a mild base to yield a diketopiperazine terminus.
Claim 10
Independent Claim
117 words
A method for preparing a derivatized peptide, said method comprising: (i) providing a support of the formula: ##STR5## wherein (X) is a solid-phase peptide synthesis stable protecting group, and n is 0 or 1; (ii) coupling a protected amino acid to the free NH 2 to provide a protected oligopeptide; (iii) deprotecting the protected oligopeptide to provide a new free NH 2 ; (iv) coupling a protected amino acid to the new free NH 2 to provide a new protected oligopeptide; (v) repeating steps (iii) and (iv) until the desired peptide sequence is obtained; (vi) removing (X) to provide a deprotected amine; and (vii) treating said deprotected amine with a mild base to yield a diketopiperazine terminus.
Claim 11
Dependent Claim
25 words
The method of claim 7, wherein said spacer is selected from the group consisting of N-acetyl serine, glycolic acid, lactic acid, and p-(hydroxymethyl) benzoic acid.
Claim 12
Independent Claim
129 words
A method for preparing a derivatized peptide, said method comprising: (i) providing a support of the formula: ##STR6## wherein (X) is a protecting group, n is 0 or 1, and y is 0 or an integer of from 2 to 6 inclusive; (ii) coupling a protected amino acid to the free NH 2 to provide a protected oligopeptide; (iii) deprotecting the protected oligopeptide to provide a new free NH 2 ; (iv) coupling a protected amino acid to the new free NH 2 to provide a new protected oligopeptide; (v) repeating steps (iii) and (iv) until the desired peptide sequence is obtained; (vi) removing (X) to provide a deprotected amine; and (vii) treating said deprotected amine with a mild base to yield an --NH--(CH 2 ) y --OH terminus.
Claim 13
Independent Claim
129 words
A method for preparing a derivatized peptide, said method comprising: (i) providing a support of the formula: ##STR6## wherein (X) is a protecting group, n is 0 or 1, and y is 0 or an integer of from 2 to 6 inclusive; (ii) coupling a protected amino acid to the free NH 2 to provide a protected oligopeptide; (iii) deprotecting the protected oligopeptide to provide a new free NH 2 ; (iv) coupling a protected amino acid to the new free NH 2 to provide a new protected oligopeptide; (v) repeating steps (iii) and (iv) until the desired peptide sequence is obtained; (vi) removing (X) to provide a deprotected amine; and (vii) treating said deprotected amine with a mild base to yield an --NH--(CH 2 ) y --OH terminus.
References Cited (Backward Citations)
No citation records indexed.
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-07-23 | 2000-01-22 | 2000-07-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-07-23 | 2004-01-22 | 2004-07-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-07-23 | 2008-01-22 | 2008-07-23 | $7700.00 | Window Closed / Lapsed |