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US5527896A Expired / Lapsed US ⏱️ 0.0 years remaining

Cloning by complementation and related processes

Assignee: Cold Spring Harbor Laboratory
•
Filing: 1991-04-19
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Granted: 1996-06-18
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Expires: 2011-04-19
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Abstract

Abstract Disclosed are methods for detecting mammalian genes encoding proteins which can function in microorganisms, particularly yeast, to modify, complement, or suppress a genetic defect associated with an identifiable phenotypic alteration or characteristic in the microorganism. Disclosed also are mammalian DNA sequences cloned by the above method, as well as polypeptide products of the expression of the DNA sequences in procaryotic or eucaryotic host cells and antibody substances which are specifically immunoreactive with said expression products. More specifically, the present invention relates to methods for cloning mammalian genes which encode products which modify, complement or suppress a genetic defect in a biochemical pathway in which cAMP participates or in a biochemical pathway which is controlled, directly or indirectly, by a RAS-related protein, to products (RNA, proteins) encoded by the mammalian genes cloned in this manner, and to antibodies which can bind the encoded proteins.

Inventors

  • 👤 Michael H. Wigler
  • 👤 John J. Colicelli

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-11-05 00:57
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/46; C07K14/47; C07K14/82; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/80; C12N15/81; C12N9/00; C12N9/14; C12N9/16

Claims Analysis

Total Claims: 8
Claim 1
Independent Claim 77 words
A purified, isolated DNA encoding a RAS-related polypeptide capable of complementing a defective RAS function in yeast, said DNA comprising a DNA selected from the group consisting of the mammalian cDNA inserts present in plasmids pJC99 (A.T.C.C. 68599), pJC265 (A.T.C.C. 68598), pJC310 (A.T.C.C. 68597), pML5 (A.T.C.C. 68593), pATG16 (A.T.C.C. 68592), and pATG29 (A.T.C.C. 68591 ) and wherein said DNA encodes a polypeptide as set out in SEQ ID NO. 14, 16, 18, 28, 30, or 32, respectively.
Claim 2
Dependent Claim 77 words
A purified, isolated DNA encoding a RAS-related polypeptide capable of complementing a defective RAS function in yeast, said DNA comprising a DNA selected from the group consisting of the mammalian cDNA inserts present in plasmids pJC99 (A.T.C.C. 68599), pJC265 (A.T.C.C. 68598), pJC310 (A.T.C.C. 68597), pML5 (A.T.C.C. 68593), pATG16 (A.T.C.C. 68592), and pATG29 (A.T.C.C. 68591 ) and wherein said DNA encodes a polypeptide as set out in SEQ ID NO. 14, 16, 18, 28, 30, or 32, respectively.
Claim 3
Dependent Claim 31 words
A purified, isolated DNA encoding a RAS-related polypeptide capable of complementing a defective RAS function in yeast, which DNA hybridizes under stringent conditions to a DNA sequence according to claim 1.
Claim 4
Dependent Claim 18 words
A purified isolated DNA encoding a polypeptide according to claim 1 or 2 by means of degenerate codons.
Claim 5
Dependent Claim 68 words
A purified, isolated DNA encoding a mammalian cyclic nucleotide phosphodiesterase, said DNA comprising a DNA selected from the group consisting of the mammalian cDNA inserts present in plasmids pRATDPD (A.T.C.C. 68586), pJC44x (A.T.C.C. 68603), pTM3 (A.T.C.C. 68600), pTM72 (A.T.C.C. 68602), and pTM22 (A.T.C.C. 68601) and wherein said DNA encodes a mammalian cyclic nucleotide phosphodiesterase as set out in SEQ ID NO. 4, 12, 22, 24, or 20, respectively.
Claim 6
Dependent Claim 68 words
A purified, isolated DNA encoding a mammalian cyclic nucleotide phosphodiesterase, said DNA comprising a DNA selected from the group consisting of the mammalian cDNA inserts present in plasmids pRATDPD (A.T.C.C. 68586), pJC44x (A.T.C.C. 68603), pTM3 (A.T.C.C. 68600), pTM72 (A.T.C.C. 68602), and pTM22 (A.T.C.C. 68601) and wherein said DNA encodes a mammalian cyclic nucleotide phosphodiesterase as set out in SEQ ID NO. 4, 12, 22, 24, or 20, respectively.
Claim 7
Dependent Claim 45 words
A purified and isolated DNA encoding a mammalian cyclic nucleotide phosphodiesterase which DNA hybridizes under stringent conditions to a DNA selected from the group consisting of DNA sequences according to claim 4 and SEQ ID NOS: 33, 34, 35, 37, 39, 41, 43 and 45.
Claim 8
Dependent Claim 23 words
A purified and isolated DNA encoding a polypeptide encoded by a DNA according to claim 4 or 5 by means of degenerate codons.

References Cited (Backward Citations)

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3.5 Years (Year 4) 1999-06-18 1999-12-18 2000-06-18 $2000.00 Window Closed / Lapsed
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