US5527713A
Expired / Lapsed
US
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Anti-CD3 antibody-aminodextran conjugates for induction of T-cell activation and proliferation
Assignee: Beckman Coulter Inc
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Filing: 1995-07-19
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Granted: 1996-06-18
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Expires: 2015-07-19
Abstract
Abstract The invention describes the use of novel aminodextran compounds containing about 5-20% by weight amine groups to bind a plurality of monoclonal antibodies. The resulting antibody-aminodextran compounds may be used to induce the activation and proliferation of selected mammalian cells. Specific examples are given using an anti-CD3 monoclonal antibody conjugated two aminodextrans containing about 5% and 16%, respectively, by weight amine groups as an agent for inducing the activation and proliferation of T cells.
Inventors
- 👤 Wade E. Bolton
- 👤 John A. Maples
- 👤 Olavi Siiman
- 👤 Norma S. Kenyon
- 👤 Cynthia G. Healy
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 09:34
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/56983; G01N33/56988; A; A61; A61K; A61K47/00; A61K47/50; A61K47/51; A61K47/68; A61K47/6835; A61K47/6849; A61K47/6883; G01N33/531; G01N33/56966; G01N33/56972
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
76 words
An antibody-aminodextran conjugate for induction of T-cell activation and proliferation, consisting essentially of T-cell specific antibodies covalently bound to amine groups present on a water soluble aminodextran having about 7-20% by weight amine groups and a molecular weight of at least 100,000 daltons, wherein said antibodies are of a single type each specific for the same T-cell antigen and wherein the molar ratio of said antibodies to said aminodextran is greater than or equal to two.
Claim 2
Dependent Claim
76 words
An antibody-aminodextran conjugate for induction of T-cell activation and proliferation, consisting essentially of T-cell specific antibodies covalently bound to amine groups present on a water soluble aminodextran having about 7-20% by weight amine groups and a molecular weight of at least 100,000 daltons, wherein said antibodies are of a single type each specific for the same T-cell antigen and wherein the molar ratio of said antibodies to said aminodextran is greater than or equal to two.
Claim 3
Dependent Claim
16 words
The conjugate according to claim 1 wherein said aminodextran has about 15-17% by weight amine groups.
Claim 4
Dependent Claim
13 words
The conjugate according to claim 1 wherein said antibody is a monoclonal antibody.
Claim 5
Dependent Claim
14 words
The conjugate according to claim 3 wherein said antibody is an anti-CD3 monoclonal antibody.
Claim 6
Dependent Claim
77 words
An antibody-aminodextran conjugate for induction of T-cell activation and proliferation, consisting essentially of T-cell specific monoclonal antibodies and a water soluble aminodextran having a molecular weight of at least 100,000 daltons and about 7-20% by weight amine groups to which said antibodies are conjugated, wherein said antibodies are of a single type each specific for the same T-cell antigen and wherein the molar ratio of said antibodies to said aminodextran is greater than or equal to two.
Claim 7
Dependent Claim
77 words
An antibody-aminodextran conjugate for induction of T-cell activation and proliferation, consisting essentially of T-cell specific monoclonal antibodies and a water soluble aminodextran having a molecular weight of at least 100,000 daltons and about 7-20% by weight amine groups to which said antibodies are conjugated, wherein said antibodies are of a single type each specific for the same T-cell antigen and wherein the molar ratio of said antibodies to said aminodextran is greater than or equal to two.
Claim 8
Dependent Claim
18 words
The conjugate according to claim 5 wherein said antibody and aminodextran are covalently linked by a bridging group.
Claim 9
Dependent Claim
15 words
The conjugate according to claim 5 wherein said aminodextran contains 10-17% by weight amine groups.
Claim 10
Dependent Claim
15 words
The conjugate according to claim 7 wherein said aminodextran contains 15-17% by weight amine groups.
Claim 11
Dependent Claim
14 words
The conjugate according to claim 5 wherein said antibody is an anti-CD3 monoclonal antibody.
References Cited (Backward Citations)
US4925648A
Cited
WO1990004633A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-06-18 | 1999-12-18 | 2000-06-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-06-18 | 2003-12-18 | 2004-06-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-06-18 | 2007-12-18 | 2008-06-18 | $7700.00 | Window Closed / Lapsed |