US5521060A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Use of a sparingly soluable salt of a heteropoly acid for the determination of an analyte, a corresponding method of determination as a suitable agent therefor
Assignee: Roche Diagnostics GmbH
•
Filing: 1994-10-12
•
Granted: 1996-05-28
•
Expires: 2014-10-12
Abstract
Abstract A sparingly soluble salt of a heteropoly acid is used for the determination of an analyte which is an aromatic amine rich in electrons or together with another substance leads to such an amine. The analyte is determined by means of the heteropoly blue color formed.
Inventors
- 👤 Joachim Hoenes
- 👤 Hans Wielinger
- 👤 Volker Unkrig
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 20:19
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/34; C12Q1/48; G01N33/52; G01N33/525; Y; Y10; Y10T; Y10T436/00; Y10T436/17; Y10T436/173845; Y10T436/174614; Y10T436/175383
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
85 words
A method for determining an enzymatically-oxidizable substrate, which comprises a) oxidizing the substrate in a sample in the presence of an oxido reductase enzyme specific for the substrate and an electron acceptor which accepts electrons and forms an organic amine which is a stronger reducing agent than aniline; b) contacting the resultant organic amine with a sparingly soluble salt of a heteropoly acid capable of forming heteropoly blue with said organic amine; and c) determining the presence of the substrate by detecting heteropoly blue formation.
Claim 2
Independent Claim
85 words
A method for determining an enzymatically-oxidizable substrate, which comprises a) oxidizing the substrate in a sample in the presence of an oxido reductase enzyme specific for the substrate and an electron acceptor which accepts electrons and forms an organic amine which is a stronger reducing agent than aniline; b) contacting the resultant organic amine with a sparingly soluble salt of a heteropoly acid capable of forming heteropoly blue with said organic amine; and c) determining the presence of the substrate by detecting heteropoly blue formation.
Claim 3
Dependent Claim
16 words
The method according to claim 1, wherein the substrate is glucose, ethanol, cholesterol, lactate or NAD(P)H.
Claim 4
Dependent Claim
18 words
The method according to claim 1, wherein the electron acceptor is an aromatic nitroso compound, oxime or hydroxylamine.
References Cited (Backward Citations)
US3016292A
Cited
US4364844A
Cited
US4681841A
Cited
US4952495A
Cited
US5206147A
Cited
US5240860A
Cited
US5382523A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-05-28 | 1999-11-27 | 2000-05-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-05-28 | 2003-11-27 | 2004-05-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-05-28 | 2007-11-27 | 2008-05-28 | $7700.00 | Window Closed / Lapsed |