US5518925A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Culture slide assembly
Assignee: Becton Dickinson and Co
•
Filing: 1995-06-06
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Granted: 1996-05-21
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Expires: 2015-06-06
Abstract
Abstract The present invention is a culture slide assembly having a base member, a cooperable receptacle removably attached to the base and means for separating the receptacle from the base. Preferably, the assembly may be used for carrying out biological reactions or growth therein, such as growing tissue or cell cultures.
Inventors
- 👤 Tadeusz A. Tyndorf
- 👤 Timothy A. Stevens
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-10 02:14
CPC Classifications
G; G02; G02B; G02B21/00; G02B21/34; C; C12; C12M; C12M41/00; C12M41/30; C12M41/36
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
41 words
A culture slide apparatus comprising a base member, a receptacle comprising a plurality of chambers, means for removably attaching said receptacle to said base member and a movable lever attached to said chamber for separating said receptacle from said base member.
Claim 2
Dependent Claim
41 words
A culture slide apparatus comprising a base member, a receptacle comprising a plurality of chambers, means for removably attaching said receptacle to said base member and a movable lever attached to said chamber for separating said receptacle from said base member.
Claim 3
Dependent Claim
21 words
The apparatus of claim 1 wherein said means for removably attaching said receptacle to said base member is an adhesive material.
Claim 4
Dependent Claim
19 words
The apparatus of claim 2 wherein said adhesive provides a liquid-impermeable seal between said receptacle and said base member.
Claim 5
Dependent Claim
23 words
The apparatus of claim 1 wherein said base member comprises a planar upper surface and opposing ends which are normal to said surface.
Claim 6
Dependent Claim
13 words
The apparatus of claim 4 wherein said base member is a microscope slide.
Claim 7
Dependent Claim
40 words
The apparatus of claim 1 wherein said receptacle comprises top and bottom surfaces, forward and rearward sections and sidewalls that extend from said top surface to said bottom surface wherein said bottom surface is removably attached with said base member.
Claim 8
Dependent Claim
27 words
The apparatus of claim 1 wherein said lever comprises a rearward end and a forward end wherein said rearward and forward ends meet at a pivot position.
Claim 9
Dependent Claim
17 words
The apparatus of claim 7 wherein said lever is removably attached to said receptacle by perforated edges.
Claim 10
Dependent Claim
10 words
The apparatus of claim 1 further comprising a removable lid.
Claim 11
Dependent Claim
31 words
The apparatus of claim 1 wherein said receptacle comprises a top surface, a bottom surface and longitudinal sidewalls and opposing sidewalls that extend from said top surface to said bottom surface.
Claim 12
Dependent Claim
11 words
The apparatus of claim 10 wherein said receptacle comprises two chambers.
Claim 13
Dependent Claim
11 words
The apparatus of claim 10 wherein said receptacle comprises four chambers.
Claim 14
Dependent Claim
11 words
The apparatus of claim 10 wherein said receptacle comprises six chambers.
Claim 15
Dependent Claim
19 words
The apparatus of claim 10 further comprising a peripheral outwardly directed flange surrounding said bottom opening of said receptacle.
Claim 16
Independent Claim
105 words
A method for growing tissue cultures with an apparatus having a base member, a receptacle comprising a plurality of chambers removably attached to said base member, a movable lever attached to said receptacle and a removable lid, comprising: (a) placing a liquid tissue culture medium containing a suspension of cells in said chambers; (b) placing said lid over said chambers; (c) incubating said apparatus until a mass of tissue cells is attached to said base; (d) removing said receptacle from said base with said lever; (e) rinsing said tissue cells attached to said base; (f) staining said tissue cells; and (g) examining said tissue cells.
Claim 17
Independent Claim
105 words
A method for growing tissue cultures with an apparatus having a base member, a receptacle comprising a plurality of chambers removably attached to said base member, a movable lever attached to said receptacle and a removable lid, comprising: (a) placing a liquid tissue culture medium containing a suspension of cells in said chambers; (b) placing said lid over said chambers; (c) incubating said apparatus until a mass of tissue cells is attached to said base; (d) removing said receptacle from said base with said lever; (e) rinsing said tissue cells attached to said base; (f) staining said tissue cells; and (g) examining said tissue cells.
Claim 18
Dependent Claim
13 words
The apparatus of claim 1 wherein said base member is glass or plastic.
Claim 19
Dependent Claim
10 words
The apparatus of claim 1 wherein said receptacle is plastic.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-05-21 | 1999-11-20 | 2000-05-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-05-21 | 2003-11-20 | 2004-05-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-05-21 | 2007-11-20 | 2008-05-21 | $7700.00 | Window Closed / Lapsed |