US5514581A
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Functional recombinantly prepared synthetic protein polymer
Assignee: Protein Polymer Technologies Inc
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Filing: 1990-11-06
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Granted: 1996-05-07
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Expires: 2010-11-06
Abstract
Abstract Novel polymers are provided which are produced by recombinant techniques. The polymers are characterized by having a small repeating sequence which provides for strands capable of associating, resulting in useful structural characteristics, where the strands are joined by turns or loops which are flexible and available for interaction with the environment. Specifically, repeating groups of naturally occurring proteins such as silk are modified by introduction of an amino-acid sequence at a site which provides for a turn between strands to provide for readily available oligopeptides capable of interacting with molecules in the environment.
Inventors
- 👤 Franco A. Ferrari
- 👤 Joseph Cappello
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 11:14
CPC Classifications
A; A61; A61L; A61L17/00; A61L29/00; A61L29/04; A61L29/049; C; C07; C07K; C07K14/00; C07K14/435; C07K14/43504; C07K14/43563; C07K14/43586; C07K14/78; C07K17/00; C07K17/02; C07K17/08; C12; C12N; C12N1/00; C12N1/06; C12N15/00; C12N15/09; C12N15/11; C12N15/62; C12N15/63; C12N15/66; C12N15/70; C12N15/74; C12N15/75; C12N5/00; C12N5/0068; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/33; C07K2319/61; C12N2533/00; C12N2533/30; C12N2533/50; C12N2533/52; G; G03; G03C; G03C1/00; G03C1/005; G03C1/04; G03C1/047
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
85 words
A DNA sequence encoding a proteinaceous polymer comprising segments of repeating units of from 3-9 amino acids naturally occurring fibroin, said segments capable of assembling into aligned structures to be formable into articles, said proteinaceous polymer having a molecular weight of at least about 15 Kdal, with at least two segments joined by an intervening oligopeptide other than said repeating units, wherein said intervening oligopeptide is unaligned, said polymer has individual segments of the same or different repeating units, wherein said intervening oligopeptide comprises RGD.
Claim 2
Dependent Claim
85 words
A DNA sequence encoding a proteinaceous polymer comprising segments of repeating units of from 3-9 amino acids naturally occurring fibroin, said segments capable of assembling into aligned structures to be formable into articles, said proteinaceous polymer having a molecular weight of at least about 15 Kdal, with at least two segments joined by an intervening oligopeptide other than said repeating units, wherein said intervening oligopeptide is unaligned, said polymer has individual segments of the same or different repeating units, wherein said intervening oligopeptide comprises RGD.
Claim 3
Dependent Claim
39 words
A DNA sequence according to claim 1, wherein said segments are of from about 25 to 150 amino acids, and said intervening oligopeptide is between each of said segments and is of from about 4 to 50 amino acids.
Claim 4
Dependent Claim
20 words
A DNA sequence according to claim 1, wherein said repeating units comprise the sequence GAGAGS (SEQ ID NO:36, positions 91-96).
Claim 5
Dependent Claim
21 words
A DNA sequence according to claim 1, wherein said intervening oligopeptide includes the amino acid sequence AVTGRGDSPAS (SEQ ID NO: 15).
Claim 6
Dependent Claim
14 words
A vector comprising a replication system and a DNA sequence according to claim 1.
Claim 7
Dependent Claim
14 words
A vector according to claim 5, wherein said replication system is a prokaryotic system.
Claim 8
Dependent Claim
18 words
A prokaryotic cell comprising a vector comprising a replication system and a DNA sequence according to claim 1.
Claim 9
Dependent Claim
59 words
A DNA sequence encoding a proteinaceous polymer having a silk protein repeating unit, wherein the polymer is of at least 15 kDal, and is comprised of segments of the formula (G A G A G S) 9 D A A V T G R G D S P A S A A G Y (SEQ ID NO:36, positions 91-160).
Claim 10
Dependent Claim
59 words
A DNA sequence encoding a proteinaceous polymer having a silk protein repeating unit, wherein the polymer is of at least 15 kDal, and is comprised of segments of the formula (G A G A G S) 9 D A A V T G R G D S P A S A A G Y (SEQ ID NO:36, positions 91-160).
References Cited (Backward Citations)
US4614517A
Cited
US5243038A
Cited
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