US5514559A
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Immunologically active conjugates and method for their preparation
Assignee: Roche Diagnostics GmbH
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Filing: 1994-03-29
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Granted: 1996-05-07
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Expires: 2014-03-29
Abstract
Abstract Novel conjugate of the general formula A-L1-L2-B wherein A and B are the substances to be coupled selected from the groups of haptens, proteins, polysaccharides, soluble polystyrenes, or peptides, L1 and L2 are trivalent linkers and L1 and L2 are bound via two thioether. They can be used in immunological assays and exhibit an improved storage stability.
Inventors
- 👤 Christine Markert-Hahn
- 👤 Beatus Ofenloch-Haehnle
- 👤 Eva Hoess
- 👤 Erasmus Huber
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 09:21
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; C; C07; C07K; C07K19/00; Y; Y10; Y10S; Y10S530/00; Y10S530/807; Y10S530/81; Y10S530/811
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
55 words
A conjugate of the formula A--L.sub.1 --L.sub.2 --B wherein A and B are substances to be coupled and are selected from the group consisting of haptens, proteins, peptides, polysaccharides and polystyrenes, and L 1 and L 2 are trivalent linkers, wherein L 1 and L 2 are linked to each other by two thioether bonds.
Claim 2
Dependent Claim
55 words
A conjugate of the formula A--L.sub.1 --L.sub.2 --B wherein A and B are substances to be coupled and are selected from the group consisting of haptens, proteins, peptides, polysaccharides and polystyrenes, and L 1 and L 2 are trivalent linkers, wherein L 1 and L 2 are linked to each other by two thioether bonds.
Claim 3
Dependent Claim
25 words
The conjugate according to claim 1, wherein A and B are linked to L 1 and L 2 , respectively, by hydroxysuccinimide or carbodiimidazolyl residues.
Claim 4
Dependent Claim
30 words
The conjugate according to claim 1, wherein at least one of A and B are selected from the group consisting of antibodies, antibody fragments, enzymes, haptens, biotin, streptavidin and peroxidase.
Claim 5
Dependent Claim
38 words
An immunological assay using a bound analyte to bind to a specific binding partner thereof, the improvement comprising the use of a conjugate according to claim 1 as the bound analyte, wherein said analyte is A or B.
Claim 6
Independent Claim
138 words
A method for producing a conjugate of the formula A--L.sub.1 --L.sub.2 --B wherein A and B are substances to be coupled and are selected from the group consisting of haptens, proteins, peptides, polysaccharides and polystyrene, and L 1 and L 2 are trivalent linkers, wherein L 1 and L 2 are linked to each other by two thioether bonds, comprising the steps of linking a first substance A to be coupled to a homobidental, trifunctional linker L 1 containing two maleinimido groups to form AL 1 , linking a second substance B to be coupled to a homobidental, trifunctional linker L 2 containing two thiol groups to form BL 2 , and reacting the thiol groups of BL 2 with the maleinimido groups of AL 2 to form two thioether bonds linking AL 1 to BL 2 .
Claim 7
Independent Claim
138 words
A method for producing a conjugate of the formula A--L.sub.1 --L.sub.2 --B wherein A and B are substances to be coupled and are selected from the group consisting of haptens, proteins, peptides, polysaccharides and polystyrene, and L 1 and L 2 are trivalent linkers, wherein L 1 and L 2 are linked to each other by two thioether bonds, comprising the steps of linking a first substance A to be coupled to a homobidental, trifunctional linker L 1 containing two maleinimido groups to form AL 1 , linking a second substance B to be coupled to a homobidental, trifunctional linker L 2 containing two thiol groups to form BL 2 , and reacting the thiol groups of BL 2 with the maleinimido groups of AL 2 to form two thioether bonds linking AL 1 to BL 2 .
Claim 8
Dependent Claim
25 words
The method according to claim 5, wherein A and B are linked to L 1 and L 2 , respectively, via hydroxysuccinimide or carbodiimidazolyl groups.
Claim 9
Dependent Claim
31 words
The method according to claim 5, wherein at least one of A and B is selected from the group consisting of an antibody, an antibody fragment, an enzyme and a hapten.
Claim 10
Dependent Claim
23 words
The method according to claim 5, wherein one of A and B is selected from the group consisting of biotin, streptavidin and peroxidase.
Claim 11
Dependent Claim
26 words
The method according to claim 8, wherein the other of A and B is selected from the group consisting of antibodies, antibody fragments, enzymes and haptens.
Claim 12
Dependent Claim
21 words
The assay according to claim 4, wherein A is the analyte and B is a label or a support for A.
Claim 13
Dependent Claim
31 words
The conjugate according to claim 1, wherein at least one of A and B is selected from the group consisting of an antibody, an antibody fragment, an enzyme or a hapten.
Claim 14
Dependent Claim
23 words
The conjugate according to claim 1, wherein one of A and B is selected from the group consisting of biotin, streptavidin and peroxidase.
Claim 15
Dependent Claim
26 words
The conjugate according to claim 12, wherein the other of A and B is selected from the group consisting of antibodies, antibody fragments, enzymes and haptens.
References Cited (Backward Citations)
US5082930A
Cited
US5168057A
Cited
EP0446017A1
Cited
US5091542A
Cited
WO1992022583A2
Cited
EP0618192A1
Cited
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