Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
92 words
A method of taking out a sample of a cell-containing amniotic fluid from an amniotic cavity, said method comprising the steps of: penetrating the wall of the amniotic cavity by means of a hollow needle, extracting a volume of cell-containing amniotic fluid through the hollow needle, separating outside the amniotic cavity amniotic fluid with reduced content of cells from the extracted volume of amniotic fluid, and returning at least part of the separated fluid to the amniotic cavity immediately after the separation, whereby a sample with an increased cell concentration is retained.
Claim 2
Independent Claim
92 words
A method of taking out a sample of a cell-containing amniotic fluid from an amniotic cavity, said method comprising the steps of: penetrating the wall of the amniotic cavity by means of a hollow needle, extracting a volume of cell-containing amniotic fluid through the hollow needle, separating outside the amniotic cavity amniotic fluid with reduced content of cells from the extracted volume of amniotic fluid, and returning at least part of the separated fluid to the amniotic cavity immediately after the separation, whereby a sample with an increased cell concentration is retained.
Claim 3
Dependent Claim
29 words
A method according to claim 1, wherein the separation is performed continuously while separated amniotic fluid is continuously and simultaneously returned to the amniotic cavity through a return passage.
Claim 4
Dependent Claim
47 words
A method according to claim 1, wherein said volume of amniotic fluid is extracted in at least two part volumes, amniotic fluid is being separated from each of these part volumes after extracting same and subsequently returning separated amniotic fluid prior to extracting the next part volume.
Claim 5
Dependent Claim
27 words
A method according to claim 1, wherein amniotic fluid is separated by passing at least part of said extracted volume of amniotic fluid through a cell filter.
Claim 6
Dependent Claim
45 words
A method according to claim 4, wherein said volume of amniotic fluid is extracted by means of a syringe, amniotic fluid from the amniotic cavity being drawn into a chamber of the syringe and subsequently displaced from said chamber and returned to the amniotic cavity.
Claim 7
Dependent Claim
61 words
A method according to claim 5, wherein the cell filter is arranged within a housing of a filtering unit which is removably connected to the syringe and provided with a cone on which the hollow needle is mounted whereby amniotic fluid which is drawn into the syringe chamber and subsequently displaced therefrom is passed through the housing of the filtering unit.
Claim 8
Dependent Claim
84 words
A method according to claim 6, further comprising the steps of: separating the filtering unit from the syringe when the separated fluid has been returned to the amniotic cavity, subsequently drawing a culturing medium into the syringe chamber, reconnecting the filtering unit to the syringe, and expelling the culturing medium from the syringe chamber and through the cell filter in the housing of the filtering unit in a direction so as to flush amniotic cells from the filter by means of the culturing medium.
Claim 9
Dependent Claim
32 words
A method according to claim 7, wherein the expelling step includes turning cell filter upside down within the housing of the filtering unit prior to expelling culturing medium through the cell filter.
Claim 10
Dependent Claim
35 words
A method according to claim 7, further including the step of flushing the culturing medium containing amniotic cells from the cell filter and collecting same in a culturing flask, placing the flask in an incubator.