US5491084A
Expired / Lapsed
US
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Uses of green-fluorescent protein
Assignee: Columbia University
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Filing: 1993-09-10
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Granted: 1996-02-13
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Expires: 2013-09-10
Abstract
Abstract This invention provides a cell comprising a DNA molecule having a regulatory element from a gene, other than a gene encoding a green-fluorescent protein operatively linked to a DNA sequence encoding the green-fluorescent protein. This invention also provides a method for selecting cells expressing a protein of interest which comprises: a. introducing into the cells a DNAI molecule having DNA sequence encoding the protein of interest and DNAII molecule having DNA sequence encoding a green-fluorescent protein; b. culturing the introduced cells in conditions permitting expression of the green-fluorescent protein and the protein of interest; and c. selecting the cultured cells which express green-fluorescent protein, thereby selecting cells expressing the protein of interest. Finally, this invention provides various uses of a green-fluorescent protein.
Inventors
- 👤 Martin Chalfie
- 👤 Douglas Prasher
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 01:59
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/65; C07; C07K; C07K14/00; C07K14/435; C07K14/43504; C07K14/43595; C12Q; C12Q1/00; C12Q1/68; C12Q1/6897
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
37 words
A host cell comprising a DNA molecule having a regulatory element from a gene, other than a gene encoding an Aequorea victoria green-fluorescent protein operatively linked to a DNA sequence encoding the fluorescent Aequorea victoria green-fluorescent protein.
Claim 2
Dependent Claim
37 words
A host cell comprising a DNA molecule having a regulatory element from a gene, other than a gene encoding an Aequorea victoria green-fluorescent protein operatively linked to a DNA sequence encoding the fluorescent Aequorea victoria green-fluorescent protein.
Claim 3
Dependent Claim
26 words
A cell of claim 1, wherein the cell is selected from a group consisting of bacterial cell, yeast cell, fungal cell, plant cell or animal cell.
Claim 4
Dependent Claim
12 words
A cell of claim 1, wherein the regulatory element is a promoter.
Claim 5
Dependent Claim
14 words
A cell of claim 3, wherein the promoter is activated by a heavy metal.
Claim 6
Dependent Claim
12 words
A cell of claim 3, wherein the promoter is a P450 promoter.
Claim 7
Dependent Claim
16 words
A cell of claim 3, wherein the promoter is from a gene encoding a stress protein.
Claim 8
Dependent Claim
13 words
A cell of claim 6, wherein the stress protein is a heat-shock protein.
Claim 9
Dependent Claim
12 words
A cell of claim 1, wherein the regulatory element is an enhancer.
Claim 10
Independent Claim
86 words
A method for selecting cells expressing a protein of interest which comprises: (a) introducing into the cells a DNAI molecule having DNA sequence encoding the protein of interest and DNAII molecule having DNA sequence encoding an Aequorea victoria green-fluorescent protein; (b) culturing the introduced cells in conditions permitting expression of the Aequorea victoria green-fluorescent protein and the protein of interest; and (c) selecting the cultured cells which express Aequorea victoria green-fluorescent protein, thereby selecting cells expressing the protein of interest, wherein DNAI and DNAII are linked.
Claim 11
Independent Claim
86 words
A method for selecting cells expressing a protein of interest which comprises: (a) introducing into the cells a DNAI molecule having DNA sequence encoding the protein of interest and DNAII molecule having DNA sequence encoding an Aequorea victoria green-fluorescent protein; (b) culturing the introduced cells in conditions permitting expression of the Aequorea victoria green-fluorescent protein and the protein of interest; and (c) selecting the cultured cells which express Aequorea victoria green-fluorescent protein, thereby selecting cells expressing the protein of interest, wherein DNAI and DNAII are linked.
Claim 12
Independent Claim
102 words
A method for selecting cells expressing a protein of interest which comprises: (a) introducing into the cells a DNAI molecule having DNA sequence encoding the protein of interest and DNAII molecule having DNA sequence encoding an Aequorea victoria green-fluorescent protein; (b) culturing the introduced cells in conditions permitting expression of the Aequorea victoria green-fluorescent protein and the protein of interest; and (c) selecting the cultured cells which express Aequorea victoria green-fluorescent protein, thereby selecting cells expressing the protein of interest, wherein the cells are selected from a group consisting of yeast cells, fungal cells, insect cells, nematode cells, plant or animal cells.
Claim 13
Independent Claim
102 words
A method for selecting cells expressing a protein of interest which comprises: (a) introducing into the cells a DNAI molecule having DNA sequence encoding the protein of interest and DNAII molecule having DNA sequence encoding an Aequorea victoria green-fluorescent protein; (b) culturing the introduced cells in conditions permitting expression of the Aequorea victoria green-fluorescent protein and the protein of interest; and (c) selecting the cultured cells which express Aequorea victoria green-fluorescent protein, thereby selecting cells expressing the protein of interest, wherein the cells are selected from a group consisting of yeast cells, fungal cells, insect cells, nematode cells, plant or animal cells.
Claim 14
Independent Claim
97 words
A method for localizing a protein of interest in a cell which comprises: (a) introducing into a cell a DNA molecule having DNA sequence encoding the protein of interest linked to DNA sequence encoding an Aequorea victoria green-fluorescent protein such that the protein produced by the DNA molecule will have the protein of interest fused to the Aequorea victoria green-fluorescent protein; (b) culturing the cell in condition permitting expression of the fused protein; and (c) detecting the location of the fluorescence of the fused protein in the cell, thereby localizing a protein of interest in a cell.
Claim 15
Independent Claim
97 words
A method for localizing a protein of interest in a cell which comprises: (a) introducing into a cell a DNA molecule having DNA sequence encoding the protein of interest linked to DNA sequence encoding an Aequorea victoria green-fluorescent protein such that the protein produced by the DNA molecule will have the protein of interest fused to the Aequorea victoria green-fluorescent protein; (b) culturing the cell in condition permitting expression of the fused protein; and (c) detecting the location of the fluorescence of the fused protein in the cell, thereby localizing a protein of interest in a cell.
Claim 16
Dependent Claim
14 words
A method of claim 11, wherein the cell normally expresses the protein of interest.
References Cited (Backward Citations)
No citation records indexed.
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