US5491074A
Expired / Lapsed
US
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Association peptides
Assignee: Affymetrix Inc
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Filing: 1993-05-24
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Granted: 1996-02-13
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Expires: 2013-05-24
Abstract
Abstract Peptides that form lightly associated homodimers can be used to form dimers and multimers of other molecules and molecular motifs of interest. These association peptides can dimerize regardless of whether motifs are added to the amino-terminus of the peptide, or the carboxy terminus of the peptide, although additions to the carboxy-terminus of the association peptides require the presence of certain acidic residues.
Inventors
- 👤 Lois Aldwin
- 👤 Mark Madden
- 👤 Willem P. C. Stemmer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 06:32
CPC Classifications
C; C07; C07K; C07K7/00; C07K7/04; C07K7/06; C07K14/00; C07K14/435; C07K14/46; C07K14/47; C07K5/00; C07K5/04; C07K5/10; C07K5/1024; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/62; C12Q; C12Q1/00; C12Q1/68; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; C07K2319/00; C07K2319/20; C07K2319/23; C07K2319/24; C07K2319/40; C07K2319/42
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
139 words
A method for joining two or more peptides or proteins, said method comprising the steps of: (a) attaching an association peptide to either the N- or C-terminus of each of said peptides or proteins to be joined, to form a fusion complex for each of said peptides or proteins to be joined wherein said association peptide comprises the amino acid residue sequence SKVILF (SEQ ID NO:1 ) and wherein the peptides or proteins to be joined together are attached to said association peptide's amino-terminus or to said association peptide's carboxy terminus, provided that if the peptides or proteins are attached to said carboxy terminus, then a free carboxyl group is positioned adjacent to the F residue of said association peptide; and (b) mixing the fusion complexes resulting from step (a) under conditions that allow said association peptide to dimerize.
Claim 2
Independent Claim
139 words
A method for joining two or more peptides or proteins, said method comprising the steps of: (a) attaching an association peptide to either the N- or C-terminus of each of said peptides or proteins to be joined, to form a fusion complex for each of said peptides or proteins to be joined wherein said association peptide comprises the amino acid residue sequence SKVILF (SEQ ID NO:1 ) and wherein the peptides or proteins to be joined together are attached to said association peptide's amino-terminus or to said association peptide's carboxy terminus, provided that if the peptides or proteins are attached to said carboxy terminus, then a free carboxyl group is positioned adjacent to the F residue of said association peptide; and (b) mixing the fusion complexes resulting from step (a) under conditions that allow said association peptide to dimerize.
Claim 3
Dependent Claim
38 words
The method of claim 1, wherein said peptides or proteins to be joined are selected independently from the group of molecules consisting of antibodies, antibody light and heavy chains, catalysts, peptides, polypeptides, proteins, receptors, antibody fragments, and substrates.
Claim 4
Dependent Claim
19 words
The method of claim 1, wherein said peptides or proteins to be joined are a catalyst and a substrate.
Claim 5
Dependent Claim
32 words
The method of claim 1, wherein one of said peptides or proteins to be joined is a peptide and the other of said peptides or proteins to be joined is a protein.
Claim 6
Dependent Claim
16 words
The method of claim 1, wherein said peptides or proteins to be joined are antibody fragments.
Claim 7
Dependent Claim
19 words
The method of claim 1, wherein said peptides or proteins to be joined are antibody light and heavy chains.
Claim 8
Dependent Claim
15 words
The method of claim 1, wherein said peptides or proteins to be joined are receptors.
Claim 9
Dependent Claim
24 words
The method of claim 1, wherein said peptides or proteins to be joined are attached via a linker to said association peptide's amino terminus.
Claim 10
Dependent Claim
18 words
The method of claim 8, wherein said linker is GGPPGG (SEQ. ID NO:4) or PPGG (SEQ. ID NO:3).
Claim 11
Dependent Claim
40 words
The method of claim 1, wherein one of said peptides or proteins to be joined together is attached to said association peptide's carboxy-terminus and said association peptide also has a second peptide or protein attached to the peptide's amino terminus.
References Cited (Backward Citations)
No citation records indexed.
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| 7.5 Years (Year 8) | 2003-02-12 | 2003-08-14 | 2004-02-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-02-12 | 2007-08-14 | 2008-02-13 | $7700.00 | Window Closed / Lapsed |