US5451522A
Expired / Lapsed
US
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Genes encoding and method of expressing an enzyme: phthalyl amidase
Assignee: Eli Lilly and Co
•
Filing: 1994-07-15
•
Granted: 1995-09-19
•
Expires: 2014-07-15
Abstract
Abstract Phthalyl amidase is an enzyme previously unknown in the art that catalyzes removal of the phthalyl moiety from phthalyl-containing amides. The current invention provides DNA compounds encoding the phthalyl amidase enzyme and methods for expressing such compounds. The present invention also provides recombinant DNA vectors encoding phthalyl amidase and host cells transformed with these DNA vectors.
Inventors
- 👤 Stephen W. Queener
- 👤 Joseph M. Zock
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 03:04
CPC Classifications
C; C12; C12P; C12P7/00; C12P7/40; C12P7/44; C12N; C12N9/00; C12N9/14; C12N9/78; C12N9/80; C12P17/00; C12P17/10; C12P17/16; C12P17/18; C12P17/182; Y; Y10; Y10S; Y10S930/00; Y10S930/01; Y10S930/30
Claims Analysis
Total Claims: 44
Claim 1
Independent Claim
13 words
A DNA compound that comprises an isolated nucleotide sequence encoding SEQ ID NO:2.
Claim 2
Dependent Claim
13 words
A DNA compound that comprises an isolated nucleotide sequence encoding SEQ ID NO:2.
Claim 3
Dependent Claim
15 words
The DNA compound of claim 1 wherein said isolated nucleotide sequence is SEQ ID NO:1.
Claim 4
Dependent Claim
24 words
The DNA compound of claim 1 that further comprises a signal peptide-encoding nucleotide sequence located adjacent to and upstream from said isolated nucleotide sequence.
Claim 5
Dependent Claim
17 words
The DNA compound of claim 3 wherein said signal peptide-encoding nucleotide sequence is endogenous to Xanthobacter agilis.
Claim 6
Dependent Claim
16 words
The DNA compound of claim 3 wherein said signal peptide-encoding nucleotide sequence is SEQ ID NO:8.
Claim 7
Dependent Claim
22 words
The DNA compound of claim 1 that further comprises a transcriptional termination sequence adjacent to and downstream from said isolated nucleotide sequence.
Claim 8
Dependent Claim
15 words
The DNA compound of claim 6 wherein said transcriptional termination sequence is SEQ ID NO:10.
Claim 9
Dependent Claim
13 words
A DNA compound that comprises an isolated nucleotide sequence encoding SEQ ID NO:4.
Claim 10
Dependent Claim
13 words
A DNA compound that comprises an isolated nucleotide sequence encoding SEQ ID NO:4.
Claim 11
Dependent Claim
15 words
The DNA compound of claim 8 wherein said isolated nucleotide sequence is SEQ ID NO:3.
Claim 12
Dependent Claim
15 words
The DNA compound of claim 8 wherein said isolated nucleotide sequence is SEQ ID NO:5.
Claim 13
Dependent Claim
15 words
The DNA compound of claim 8 wherein said isolated nucleotide sequence is SEQ ID NO:6.
Claim 14
Dependent Claim
13 words
A recombinant DNA vector that comprises said isolated nucleotide sequence of claim 1.
Claim 15
Dependent Claim
25 words
The recombinant DNA vector of claim 12 that further comprises a promotor and translational activating sequence positioned to drive expression of said isolated nucleotide sequence.
Claim 16
Dependent Claim
15 words
The recombinant DNA vector of claim 13 wherein said promotor is endogenous to Xanthobacter agilis.
Claim 17
Dependent Claim
14 words
The recombinant DNA vector of claim 13 wherein said promotor is SEQ ID NO:7.
Claim 18
Dependent Claim
16 words
The recombinant DNA vector of claim 13 wherein said isolated nucleotide sequence is SEQ ID NO:1.
Claim 19
Dependent Claim
16 words
The recombinant DNA vector of claim 13 wherein said isolated nucleotide sequence is SEQ ID NO:3.
Claim 20
Dependent Claim
16 words
The recombinant DNA vector of claim 13 wherein said isolated nucleotide sequence is SEQ ID NO:5.
Claim 21
Dependent Claim
16 words
The recombinant DNA vector of claim 13 wherein said isolated nucleotide sequence is SEQ ID NO:6.
Claim 22
Dependent Claim
11 words
The recombinant DNA vector of claim 13 that is plasmid pZPA600.
Claim 23
Dependent Claim
11 words
The recombinant DNA vector of claim 13 that is plasmid pZPA400.
Claim 24
Dependent Claim
12 words
A bacterial host cell transformed with said DNA vector of claim 13.
Claim 25
Dependent Claim
12 words
A bacterial host cell transformed with said DNA vector of claim 16.
Claim 26
Dependent Claim
12 words
A bacterial host cell transformed with said DNA vector of claim 17.
Claim 27
Dependent Claim
12 words
A bacterial host cell transformed with said DNA vector of claim 18.
Claim 28
Dependent Claim
12 words
A bacterial host cell transformed with said DNA vector of claim 19.
Claim 29
Dependent Claim
12 words
A bacterial host cell transformed with said DNA vector of claim 20.
Claim 30
Dependent Claim
12 words
A bacterial host cell transformed with said DNA vector of claim 21.
Claim 31
Dependent Claim
18 words
The bacterial host cell of claim 22 selected from the group consisting of Escherichia coli and Streptomyces lividans.
Claim 32
Dependent Claim
11 words
The bacterial host cell of claim 29 that is Streptomyces lividans/pZPA600.
Claim 33
Dependent Claim
11 words
The bacterial host cell of claim 29 that is Escherichia coli/pZPA400.
Claim 34
Dependent Claim
32 words
A method for producing recombinant phthalyl amidase, said method comprising culturing a host cell of claim 22 under conditions suitable for gene expression, and recovering the expressed phthalyl amidase from the culture.
Claim 35
Dependent Claim
32 words
A method for producing recombinant phthalyl amidase, said method comprising culturing a host cell of claim 23 under conditions suitable for gene expression, and recovering the expressed phthalyl amidase from the culture.
Claim 36
Dependent Claim
32 words
A method for producing recombinant phthalyl amidase, said method comprising culturing a host cell of claim 24 under conditions suitable for gene expression, and recovering the expressed phthalyl amidase from the culture.
Claim 37
Dependent Claim
32 words
A method for producing recombinant phthalyl amidase, said method comprising culturing a host cell of claim 25 under conditions suitable for gene expression, and recovering the expressed phthalyl amidase from the culture.
Claim 38
Dependent Claim
32 words
A method for producing recombinant phthalyl amidase, said method comprising culturing a host cell of claim 26 under conditions suitable for gene expression, and recovering the expressed phthalyl amidase from the culture.
Claim 39
Dependent Claim
32 words
A method for producing recombinant phthalyl amidase, said method comprising culturing a host cell of claim 27 under conditions suitable for gene expression, and recovering the expressed phthalyl amidase from the culture.
Claim 40
Dependent Claim
32 words
A method for producing recombinant phthalyl amidase, said method comprising culturing a host cell of claim 28 under conditions suitable for gene expression, and recovering the expressed phthalyl amidase from the culture.
Claim 41
Dependent Claim
32 words
A method for producing recombinant phthalyl amidase, said method comprising culturing a host cell of claim 29 under conditions suitable for gene expression, and recovering the expressed phthalyl amidase from the culture.
Claim 42
Dependent Claim
32 words
A method for producing recombinant phthalyl amidase, said method comprising culturing a host cell of claim 30 under conditions suitable for gene expression, and recovering the expressed phthalyl amidase from the culture.
Claim 43
Dependent Claim
32 words
A method for producing recombinant phthalyl amidase, said method comprising culturing a host cell of claim 31 under conditions suitable for gene expression, and recovering the expressed phthalyl amidase from the culture.
Claim 44
Dependent Claim
15 words
The DNA compound of claim 1 wherein said isolated nucleotide sequence is SEQ ID NO:11.
References Cited (Backward Citations)
US5032510A
Cited
US4977089A
Cited
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1998-09-18 | 1999-03-20 | 1999-09-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-09-18 | 2003-03-20 | 2003-09-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-09-18 | 2007-03-20 | 2007-09-19 | $7700.00 | Window Closed / Lapsed |