US5437997A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Carotenoid producing culture using Nespongiococcum excentricum
Assignee: ZGI II Inc
•
Filing: 1990-02-05
•
Granted: 1995-08-01
•
Expires: 2010-02-05
Abstract
Abstract A culture for the production of xanthophylls comprising Nospongiococcum excentricum ATCC 40335 and mutants thereof. The culture having a dry cell weight xanthophyll content of at least about 0.65% and being capable of growing to a cell density of greater than about 40 grams per liter. The culture also comprising: carbon, phosphate, sulfate, iron, magnesium, and nitrogen.
Inventors
- 👤 Hans H. Liao
- 👤 Richard D. Medwid
- 👤 Donald L. Heefner
- 👤 Kathleen S. Sniff
- 👤 Randal A. Hassler
- 👤 Michael J. Yarus
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 12:27
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/12; C12N1/125; A; A01; A01H; A01H13/00; C12P; C12P23/00; C12R; C12R2001/00; C12R2001/89
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
51 words
An alga having the identifying characteristics of Neospongiococcum excentricum ATCC Accession No. 40335 and mutants thereof having said identifying characteristics, wherein said identifying characteristics comprise having a dry weight xanthophyll content of at least about 0.65% and being capable of growing to a cell density of greater than about 40 g/l.
Claim 2
Dependent Claim
51 words
An alga having the identifying characteristics of Neospongiococcum excentricum ATCC Accession No. 40335 and mutants thereof having said identifying characteristics, wherein said identifying characteristics comprise having a dry weight xanthophyll content of at least about 0.65% and being capable of growing to a cell density of greater than about 40 g/l.
Claim 3
Dependent Claim
148 words
A culture for the production of xanthophylls, said culture comprising: a) an alga having the identifying characteristics of Neospongiococcum excentricum ATCC 40335 and mutants thereof having said identifying characteristics, wherein said identifying characteristics comprising having a dry cell weight xanthophyll content of at least about 0.65% and being capable of growing to a cell density of greater than about 40 g/l; b) a carbon source in a concentration between 5 g/l and 30 g/l; c) a phosphate source having a molar concentration of phosphate of between 16 mM and 33 mM; d) a sulfate source having a molar concentration of sulfate of between 15 mM and 30 mM; e) an iron source having a molar concentration of iron of between 0.270 mM and 0.504 mM; f) a magnesium source having a molar concentration of magnesium of between 2.0 mM and 7.0 mM; and g) a source of nitrogen.
Claim 4
Dependent Claim
148 words
A culture for the production of xanthophylls, said culture comprising: a) an alga having the identifying characteristics of Neospongiococcum excentricum ATCC 40335 and mutants thereof having said identifying characteristics, wherein said identifying characteristics comprising having a dry cell weight xanthophyll content of at least about 0.65% and being capable of growing to a cell density of greater than about 40 g/l; b) a carbon source in a concentration between 5 g/l and 30 g/l; c) a phosphate source having a molar concentration of phosphate of between 16 mM and 33 mM; d) a sulfate source having a molar concentration of sulfate of between 15 mM and 30 mM; e) an iron source having a molar concentration of iron of between 0.270 mM and 0.504 mM; f) a magnesium source having a molar concentration of magnesium of between 2.0 mM and 7.0 mM; and g) a source of nitrogen.
Claim 5
Dependent Claim
67 words
A culture as claimed in claim 2, wherein said carbon source comprises glucose, wherein said phosphate source comprises KH 2 PO 4 , wherein said sulfate source comprises (NH 4 ) 2 SO 4 , MgSO 4 .7H 2 O, and FeSO 4 .7H 2 O, wherein said magnesium source comprises MgSO 4 .7H 2 O, and wherein said iron source comprises FeSO 4 .7H 2 O.
Claim 6
Dependent Claim
26 words
A culture as claimed in claim 2, further comprising trace amounts of copper, calcium, and zinc, and wherein said nitrogen source comprises a complex nitrogen source.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-07-31 | 1999-01-30 | 1999-08-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-07-31 | 2003-01-30 | 2003-08-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-07-31 | 2007-01-30 | 2007-08-01 | $7700.00 | Window Closed / Lapsed |