US5422264A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Soluble factor stimulation of attachment and hemidesmosome assembly in epithelial cells
Assignee: KAHN RAYMOND
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Filing: 1993-11-12
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Granted: 1995-06-06
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Expires: 2013-11-12
Abstract
Abstract A method for growing epithelial cells in vitro using soluble proteins secreted by 804G rat bladder carcinoma cells. These proteins are able to stimulate cell attachment and hemidesmosome formation in cells grown in contact with the proteins. The purification of these proteins from 804G culture supernatant is greatly facilitated by culturing the cells under low serum conditions.
Inventors
- 👤 Vito Quaranta
- 👤 Marketta Hormia
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 20:52
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0625; C12N5/0629; A; A61; A61L; A61L27/00; A61L27/14; A61L27/22; A61L27/227; A61L27/28; A61L27/34; A61L27/36; A61L27/38; A61L27/3895; A61L29/00; A61L29/08; A61L29/085; A61L31/00; A61L31/08; A61L31/10; C07; C07K; C07K14/00; C07K14/435; C07K14/46; C07K14/47; C07K14/4701; C07K14/4702; C07K14/4705; C07K14/78; C07K16/00; C07K16/18; C12N5/0618; C12N5/0621; C12N2501/00; C12N2501/50; C12N2501/58; C12N2502/00; C12N2502/25; C12N2502/253; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/828; Y10S530/834
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
59 words
A method for culturing mammalian epithelial cells in vitro comprising: culturing 804G rat bladder carcinoma cells in media under conditions that promote the secretion of soluble factors, wherein said 804G cells secrete said factors; removing said media from said 804G cells; and culturing said media with said epithelial cells, under conditions effective for hemidesmosome formation in said epithelial cells.
Claim 2
Independent Claim
59 words
A method for culturing mammalian epithelial cells in vitro comprising: culturing 804G rat bladder carcinoma cells in media under conditions that promote the secretion of soluble factors, wherein said 804G cells secrete said factors; removing said media from said 804G cells; and culturing said media with said epithelial cells, under conditions effective for hemidesmosome formation in said epithelial cells.
Claim 3
Dependent Claim
11 words
The method of claim 1 wherein said mammalian cells are human.
Claim 4
Dependent Claim
13 words
The method of claim 1 wherein said epithelial cells are human skin cells.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-06-05 | 1998-12-05 | 1999-06-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-06-05 | 2002-12-05 | 2003-06-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-06-05 | 2006-12-05 | 2007-06-06 | $7700.00 | Window Closed / Lapsed |