US5421339A
Expired / Lapsed
US
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Diagnosis of dysplasia using laser induced fluoroescence
Assignee: The University of Texas System
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Filing: 1993-05-12
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Granted: 1995-06-06
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Expires: 2013-05-12
Abstract
Abstract Apparatus and in vivo methods to distinguish normal and abnormal cervical tissue and to detect cervical intraepithelial neoplasia (CIN) in a diagnostic cervical tissue sample. Induced fluorescence intensity spectra from known normal cervical tissue and a diagnostic tissue sample are obtained from the same patient. Peak fluorescence intensity values for normal tissue samples are averaged, as are slope measurements from predetermined portions of spectra induced in both known normal cervical tissue and the diagnostic tissue sample. Peak fluorescence intensities of diagnostic tissue spectra are divided by average peak fluorescence intensity values for normal tissue in the same patient to yield relative peak fluorescence intensity values. Normal and abnormal cervical tissues are distinguished using a predetermined empirical discriminant function of slope measurements derived from normal tissue spectra and relative peak fluorescence intensity measurements in the same patient. CIN is distinguished from tissue with human papilloma virus injection or inflammation using a predetermined empirical discriminant function of average slope measurements on spectra from known normal tissue and slope measurements on a diagnostic tissue spectrum.
Inventors
- 👤 Nirmala Ramanujam
- 👤 Anita Mahadevan
- 👤 Rebecca R. Richards-Kortum
- 👤 Michele F. Mitchell
- 👤 Sharon Thomsen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 11:40
CPC Classifications
A; A61; A61B; A61B5/00; A61B5/0059; A61B5/0082; A61B5/0084; A61B5/0071; A61B5/0075; A61B5/43; A61B5/4306; A61B5/4318; A61B5/4331; G; G01; G01N; G01N21/00; G01N21/62; G01N21/63; G01N21/64; G01N21/6402; G01N2021/6417
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
125 words
An in vivo method of detecting tissue abnormality in a diagnostic cervical tissue sample in a patient, comprising: illuminating the diagnostic cervical tissue sample and a presumptively histologically normal cervical tissue sample with electromagnetic radiation; detecting a first fluorescence intensity spectrum from the diagnostic tissue sample; detecting a second fluorescence intensity spectrum from the presumptively histologically normal cervical tissue sample; calculating from a predetermined portion of said first fluorescence intensity spectrum, a first slope parameter which is indicative of tissue abnormality; calculating from a predetermined portion of said second fluorescence intensity spectrum, a second slope parameter which is indicative of presumptively histologically normal cervical tissue; and detecting tissue abnormality in the diagnostic cervical tissue sample as a function of said first and second slope parameters.
Claim 2
Dependent Claim
125 words
An in vivo method of detecting tissue abnormality in a diagnostic cervical tissue sample in a patient, comprising: illuminating the diagnostic cervical tissue sample and a presumptively histologically normal cervical tissue sample with electromagnetic radiation; detecting a first fluorescence intensity spectrum from the diagnostic tissue sample; detecting a second fluorescence intensity spectrum from the presumptively histologically normal cervical tissue sample; calculating from a predetermined portion of said first fluorescence intensity spectrum, a first slope parameter which is indicative of tissue abnormality; calculating from a predetermined portion of said second fluorescence intensity spectrum, a second slope parameter which is indicative of presumptively histologically normal cervical tissue; and detecting tissue abnormality in the diagnostic cervical tissue sample as a function of said first and second slope parameters.
Claim 3
Dependent Claim
33 words
The method of claim 1, said illuminating step comprising, illuminating said diagnostic cervical tissue sample and said presumptively histologically normal cervical tissue sample with electromagnetic radiation having a wavelength of about 337 nm.
Claim 4
Dependent Claim
39 words
The method of claim 1, said step of calculating said first slope parameter comprising, calculating said first slope parameter from a portion of said first fluorescence intensity spectrum corresponding to wavelengths between about 440 nm and about 460 nm.
Claim 5
Dependent Claim
39 words
The method of claim 1, said step of calculating said second slope parameter comprising, calculating said second slope parameter from a portion of said second fluorescence intensity spectrum corresponding to wavelengths between about 410 nm and about 430 nm.
Claim 6
Dependent Claim
39 words
The method of claim 1, said step of calculating said first slope parameter comprising, calculating said first slope parameter after normalization of said first fluorescence intensity spectrum with a peak fluorescence intensity value of said first fluorescence intensity spectrum.
Claim 7
Dependent Claim
28 words
The method of claim 5, wherein said first slope parameter is a function of an average slope in said predetermined portion of said normalized first fluorescence intensity spectrum.
Claim 8
Dependent Claim
39 words
The method of claim 1, said step of calculating said second slope parameter comprising, calculating said second slope parameter after normalization of said second fluorescence intensity spectrum with a peak fluorescence intensity value of said second fluorescence intensity spectrum.
Claim 9
Dependent Claim
28 words
The method of claim 7, wherein said second slope parameter is a function of an average slope in said predetermined portion of said normalized second fluorescence intensity spectrum.
Claim 10
Dependent Claim
28 words
The method of claim 1, said step of detecting tissue abnormality comprising, detecting tissue abnormality as a predetermined empirical discriminant function of said first and second slope parameters.
Claim 11
Dependent Claim
29 words
The method of claim 9, said step of detecting tissue abnormality comprising, detecting tissue abnormality as a predetermined linear empirical discriminant function of said first and second slope parameters.
Claim 12
Dependent Claim
128 words
An in vivo method of detecting tissue abnormality in a diagnostic cervical tissue sample in a patient, comprising: identifying a presumptively histologically normal cervical tissue sample from the patient; illuminating the diagnostic tissue sample and said presumptively histologically normal tissue sample with electromagnetic radiation; detecting a first fluorescence intensity spectrum from the diagnostic tissue sample; detecting a second fluorescence intensity spectrum from said presumptively histologically normal tissue sample; calculating a slope parameter indicative of tissue abnormality from said first fluorescence intensity spectrum; calculating an intensity parameter characteristic of normal tissue from said second fluorescence intensity spectrum; calculating a discriminant parameter as a function of said slope and intensity parameters; detecting tissue abnormality in the diagnostic cervical tissue sample by substituting said discriminant parameter in a predetermined discriminant function.
Claim 13
Dependent Claim
128 words
An in vivo method of detecting tissue abnormality in a diagnostic cervical tissue sample in a patient, comprising: identifying a presumptively histologically normal cervical tissue sample from the patient; illuminating the diagnostic tissue sample and said presumptively histologically normal tissue sample with electromagnetic radiation; detecting a first fluorescence intensity spectrum from the diagnostic tissue sample; detecting a second fluorescence intensity spectrum from said presumptively histologically normal tissue sample; calculating a slope parameter indicative of tissue abnormality from said first fluorescence intensity spectrum; calculating an intensity parameter characteristic of normal tissue from said second fluorescence intensity spectrum; calculating a discriminant parameter as a function of said slope and intensity parameters; detecting tissue abnormality in the diagnostic cervical tissue sample by substituting said discriminant parameter in a predetermined discriminant function.
Claim 14
Dependent Claim
130 words
An in vivo method of detecting CIN in a diagnostic cervical tissue sample in a patient having presumptively known normal cervical tissue, comprising: illuminating the diagnostic tissue sample and presumptively known normal cervical tissue with electromagnetic radiation; detecting a first fluorescence intensity spectrum from the diagnostic tissue sample; detecting a second fluorescence intensity spectrum from the presumptively known normal cervical tissue; calculating from a predetermined portion of said first fluorescence intensity spectrum a first slope parameter which is indicative of CIN in the diagnostic cervical tissue sample; calculating from a predetermined portion of said second fluorescence intensity spectrum a second slope parameter which characterizes presumptively normal cervical tissue in the patient; and detecting CIN in the diagnostic cervical tissue sample as a function of said first and second slope parameters.
Claim 15
Dependent Claim
130 words
An in vivo method of detecting CIN in a diagnostic cervical tissue sample in a patient having presumptively known normal cervical tissue, comprising: illuminating the diagnostic tissue sample and presumptively known normal cervical tissue with electromagnetic radiation; detecting a first fluorescence intensity spectrum from the diagnostic tissue sample; detecting a second fluorescence intensity spectrum from the presumptively known normal cervical tissue; calculating from a predetermined portion of said first fluorescence intensity spectrum a first slope parameter which is indicative of CIN in the diagnostic cervical tissue sample; calculating from a predetermined portion of said second fluorescence intensity spectrum a second slope parameter which characterizes presumptively normal cervical tissue in the patient; and detecting CIN in the diagnostic cervical tissue sample as a function of said first and second slope parameters.
Claim 16
Dependent Claim
24 words
The method of claim 12, said illuminating step comprising, illuminating said diagnostic tissue sample with electromagnetic radiation having a wavelength of about 337 nm.
Claim 17
Dependent Claim
38 words
The method of claim 12, said step of calculating said first slope parameter comprising, calculating said first slope parameter from a portion of said fluorescence intensity spectrum corresponding to wavelengths between about 440 nm and about 460 nm.
Claim 18
Dependent Claim
39 words
The method of claim 12, said step of calculating said second slope parameter comprising, calculating said second slope parameter from a portion of said second fluorescence intensity spectrum corresponding to wavelengths between about 410 nm and about 430 nm.
Claim 19
Dependent Claim
31 words
The method of claim 12, wherein said first and second slope parameters are calculated after normalization of said first and second fluorescence intensity spectra to peak fluorescence intensity values of 1.
Claim 20
Dependent Claim
64 words
The method of claim 14, wherein said first and second slope parameters are functions of average slopes in said normalized first and second fluorescence intensity spectra respectively, said slopes being measured in predetermined regions of said normalized first and second fluorescence intensity spectra respectively, said regions lying substantially between wavelengths corresponding to said predetermined portions of said first and second fluorescence intensity spectra respectively.
Claim 21
Dependent Claim
26 words
The method of claim 12, said step of detecting CIN comprising, detecting CIN as a predetermined empirical discriminant function of said first and second slope parameters.
Claim 22
Dependent Claim
27 words
The method of claim 18, said step of detecting CIN comprising, detecting CIN as a predetermined linear empirical discriminant function of said first and second slope parameters.
Claim 23
Independent Claim
211 words
An apparatus for in vivo detection of tissue abnormality in a diagnostic cervical tissue sample in a patient having presumptively known normal cervical tissue, comprising: means for applying electromagnetic radiation to a diagnostic cervical tissue sample and to presumptively known normal cervical tissue; radiation detection means for detecting a fluorescence intensity spectrum induced in the diagnostic cervical tissue sample, and for detecting a plurality of normal fluorescence intensity spectra induced in the presumptively known normal cervical tissue by said means for applying; a computer, connected to said means for applying and to said means for detecting, including, means for measuring a peak normal fluorescence intensity value in each normal fluorescence intensity spectrum, means for calculating an average peak normal fluorescence intensity from said peak normal fluorescence intensity values, means for measuring a peak fluorescence intensity value from said fluorescence intensity spectrum induced in the diagnostic cervical tissue sample, means for calculating from a predetermined portion of said fluorescence intensity spectrum a slope parameter, which is indicative of tissue abnormality, means for calculating relative peak fluorescence intensity as a function of said peak fluorescence intensity value and said average peak normal fluorescence intensity, and means for detecting tissue abnormality as a function of said slope parameter and said relative peak fluorescence intensity.
Claim 24
Independent Claim
211 words
An apparatus for in vivo detection of tissue abnormality in a diagnostic cervical tissue sample in a patient having presumptively known normal cervical tissue, comprising: means for applying electromagnetic radiation to a diagnostic cervical tissue sample and to presumptively known normal cervical tissue; radiation detection means for detecting a fluorescence intensity spectrum induced in the diagnostic cervical tissue sample, and for detecting a plurality of normal fluorescence intensity spectra induced in the presumptively known normal cervical tissue by said means for applying; a computer, connected to said means for applying and to said means for detecting, including, means for measuring a peak normal fluorescence intensity value in each normal fluorescence intensity spectrum, means for calculating an average peak normal fluorescence intensity from said peak normal fluorescence intensity values, means for measuring a peak fluorescence intensity value from said fluorescence intensity spectrum induced in the diagnostic cervical tissue sample, means for calculating from a predetermined portion of said fluorescence intensity spectrum a slope parameter, which is indicative of tissue abnormality, means for calculating relative peak fluorescence intensity as a function of said peak fluorescence intensity value and said average peak normal fluorescence intensity, and means for detecting tissue abnormality as a function of said slope parameter and said relative peak fluorescence intensity.
References Cited (Backward Citations)
US4786813A
Cited
US5125404A
Cited
US5318024A
Cited
US4930516A
Cited
US5042494A
Cited
US4930516B1
Cited
US5046501A
Cited
US5115137A
Cited
US5201318A
Cited
US5174297A
Cited
US5131398A
Cited
US5168162A
Cited
US5261410A
Cited
US5303026A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1998-06-05 | 1998-12-05 | 1999-06-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-06-05 | 2002-12-05 | 2003-06-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-06-05 | 2006-12-05 | 2007-06-06 | $7700.00 | Window Closed / Lapsed |