Patent US3160157A successfully refreshed from Google Patents.
US5419323A
Expired / Lapsed
US
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Method for laser induced fluorescence of tissue
Assignee: Massachusetts Institute of Technology
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Filing: 1989-11-17
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Granted: 1995-05-30
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Expires: 2009-11-17
Abstract
Abstract A method for laser induced fluorescence of tissue in which laser radiation is used to illuminate and induce fluorescence in the tissue under study to determine the chemical composition or pathologic condition of tissue. The laser radiation and the retrieved fluorescing radiation can be conveyed through a catheter using an array of optical fiber. The fluorescence spectrum of the tissue can be displayed and analyzed to obtain information regarding the chemical composition and medical condition of the tissue inside the human body.
Inventors
- 👤 Carter Kittrell
- 👤 Robert M. Cothren
- 👤 Michael S. Feld
- 👤 Joseph J. Baraga
- 👤 Kyungwon An
- 👤 Rebecca Richards-Kortum
- 👤 Richard P. Rava
- 👤 Young D. Park
- 👤 Anand Mehta
- 👤 Paola Taroni
- 👤 Lucene Tong
- 👤 Ramachandra R. Dasari
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 11:40
CPC Classifications
A; A61; A61B; A61B5/00; A61B5/0059; A61B5/0082; A61B5/0084; A61B18/00; A61B18/18; A61B18/20; A61B18/22; A61B18/24; A61B18/245; A61B5/0071; A61B5/0075; A61B5/145; A61B5/1455; A61B5/1459; A61B2562/00; A61B2562/02; A61B2562/0233; A61B2562/0238
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
91 words
A method of irradiating chromophores in endogenous tissue to identify tissue composition comprising: selecting a known excitation wavelength of the chromophore within a range of wavelengths at which the chromophore undergoes fluorescence; irradiating the endogeneous tissue with radiation having at least the selected excitation wavelength within the range; detecting a fluorescence emission of the tissue resulting from the irradiation thereof; analyzing the detected emission to determine the presence of the chromophore in the tissue; forming an attenuation spectrum for a tissue component; and modulating the detected emission with the attenuation spectrum.
Claim 2
Independent Claim
91 words
A method of irradiating chromophores in endogenous tissue to identify tissue composition comprising: selecting a known excitation wavelength of the chromophore within a range of wavelengths at which the chromophore undergoes fluorescence; irradiating the endogeneous tissue with radiation having at least the selected excitation wavelength within the range; detecting a fluorescence emission of the tissue resulting from the irradiation thereof; analyzing the detected emission to determine the presence of the chromophore in the tissue; forming an attenuation spectrum for a tissue component; and modulating the detected emission with the attenuation spectrum.
Claim 3
Dependent Claim
28 words
The method of irradiating chromophores of claim 1 wherein the step of forming the attenuation spectrum comprises detecting absorption and scattering components of light incident upon the tissue.
Claim 4
Dependent Claim
75 words
The method of irradiating chromophores of claim 1 further comprising the steps of: selecting a second known excitation wavelength of a second chromophore at which the second chromophore undergoes fluorescence; irradiating simultaneously the endogeneous tissue with radiation having the second wavelength; detecting a second fluorescence emission of the tissue resulting from the irradiation within the second wavelength; and analyzing the second detected fluorescence emission to determine the presence of the second chromophore in the tissue.
Claim 5
Dependent Claim
28 words
The method of claim 3 further comprising illuminating the tissue at additional selected wavelengths to induce fluorescence of additional chromophores and determining additional pathological characteristics of the tissue.
Claim 6
Dependent Claim
31 words
The method of irradiating chromophores of claim 3 further comprising the step of determining the wavelength of peak fluorescence of the first and second chromophores within the first and second emissions.
Claim 7
Dependent Claim
21 words
The method of irradiating chromophores of claim 5 further comprising the step of determining the ratio of the two fluorescence peaks.
Claim 8
Dependent Claim
39 words
The method of irradiating chromophores of claim 1 further comprising the step of determining the wavelength of peak fluorescence of the chromophore within the emission and determining the ratio of the peak with a selected valley within the emission.
Claim 9
Dependent Claim
19 words
The method of irradiating chromophores of claim 1 further comprising determining the concentration of the chromophore within the tissue.
Claim 10
Dependent Claim
69 words
The method of claim 1 further comprising: inserting a catheter containing a multiplicity of optical fibers into an artery in the body and positioning a distal end of said catheter adjacent to tissue to be diagnosed; and coupling a light source to at least one of said fibers to direct light out of a distal end of the optical fiber or fibers to which the light source is coupled.
Claim 11
Dependent Claim
16 words
The method of irradiating chromophores of claim 1 wherein the chromophore comprises tryptophan in arterial tissue.
Claim 12
Dependent Claim
13 words
The method of irradiating chromophores of claim 1 wherein the chromophore comprises collagen.
Claim 13
Dependent Claim
13 words
The method of irradiating chromophores of claim 1 wherein the chromophore comprises elastin.
Claim 14
Independent Claim
87 words
A method of irradiating chromophores in endogenous tissue to identify tissue composition comprising: selecting a known excitation wavelength of the chromophore within a range of wavelengths at which the chromophore undergoes fluorescence; irradiating the endogeneous tissue with radiation having at least the selected excitation wavelength within the range; detecting a fluorescence emission of the tissue resulting from the irradiation thereof; analyzing the detected emission to determine the presence of the chromophore in the tissue, including separating a component of the emission resulting from reabsorption by the tissue.
Claim 15
Independent Claim
87 words
A method of irradiating chromophores in endogenous tissue to identify tissue composition comprising: selecting a known excitation wavelength of the chromophore within a range of wavelengths at which the chromophore undergoes fluorescence; irradiating the endogeneous tissue with radiation having at least the selected excitation wavelength within the range; detecting a fluorescence emission of the tissue resulting from the irradiation thereof; analyzing the detected emission to determine the presence of the chromophore in the tissue, including separating a component of the emission resulting from reabsorption by the tissue.
Claim 16
Dependent Claim
27 words
The method of irradiating chromophores of claim 13 further comprising forming an attenuation spectrum for a tissue component; and modulating the detected emission with the attenuation spectrum.
Claim 17
Dependent Claim
28 words
The method of irradiation chromophores of claim 14 wherein the step of forming the attenuation spectrum comprises detecting absorption and scattering components of light incident upon the tissue.
Claim 18
Dependent Claim
74 words
The method of irradiating chromophores of claim 13 further comprising the steps of: selecting a second known excitation wavelength of a second chromophore at which the second chromophore undergoes fluorescence; irradiating simultaneously the endogeneous tissue with radiation having the second wavelength; detecting a second fluorescence emission of the tissue resulting from the irradiation within the second wavelength; and analyzing the second detected emission to determine the presence of the second chromophore in the tissue.
Claim 19
Independent Claim
100 words
A method of irradiating chromophores in endogenous tissue to identify tissue composition comprising: selecting a known excitation wavelength of the chromophore within a range of wavelengths at which the chromophore undergoes fluorescence; inserting a fiber optic probe into the body of a patient; irradiating the endogeneous tissue with the fiber optic probe using radiation having at least the selected excitation wavelength within the range; detecting a fluorescence emission of the tissue resulting from the irradiation thereof; analyzing the detected emission to determine the presence of the chromophore in the tissue; and determining the concentration of the chromophore within the tissue.
Claim 20
Independent Claim
100 words
A method of irradiating chromophores in endogenous tissue to identify tissue composition comprising: selecting a known excitation wavelength of the chromophore within a range of wavelengths at which the chromophore undergoes fluorescence; inserting a fiber optic probe into the body of a patient; irradiating the endogeneous tissue with the fiber optic probe using radiation having at least the selected excitation wavelength within the range; detecting a fluorescence emission of the tissue resulting from the irradiation thereof; analyzing the detected emission to determine the presence of the chromophore in the tissue; and determining the concentration of the chromophore within the tissue.
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| 7.5 Years (Year 8) | 2002-05-29 | 2002-11-28 | 2003-05-30 | $3760.00 | Window Closed / Lapsed |
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