US5415758A — Method and apparatus for electro-elution of biolo…

Claim 1

Independent Claim 83 words

An electro-elution apparatus comprising plural sample chambers, each sample chamber containing an eluant solution; an anode wire and a cathode wire, wherein one of said anode or cathode wires comprises a plurality of serially connected conductive extensions, and each said conductive extension extends into a corresponding sample chamber, and wherein each said sample chamber defines a flow path for flow of electrolytes between said cathode and anode wires, wherein said plural sample chambers are simultaneously accessible for simultaneous removal of said eluant solutions.

Claim 2

Dependent Claim 83 words

An electro-elution apparatus comprising plural sample chambers, each sample chamber containing an eluant solution; an anode wire and a cathode wire, wherein one of said anode or cathode wires comprises a plurality of serially connected conductive extensions, and each said conductive extension extends into a corresponding sample chamber, and wherein each said sample chamber defines a flow path for flow of electrolytes between said cathode and anode wires, wherein said plural sample chambers are simultaneously accessible for simultaneous removal of said eluant solutions.

Claim 3

Dependent Claim 48 words

The electro-elution apparatus of claim 1, further comprising a buffer reservoir for containing said plural sample chambers, wherein each said sample chamber comprises upper and lower portions, said lower portion comprising barrier means for allowing electrolyte flow but preventing liquid exchange between each said chamber and said reservoir.

Claim 4

Dependent Claim 34 words

The electro-elution apparatus of claim 2 wherein said anode wire comprises said plurality of serially connected conductive extensions for extending into each corresponding sample chamber, and said cathode wire extends into said buffer reservoir.

Claim 5

Dependent Claim 43 words

A method of electro-elution, comprising providing plural electric fields using the apparatus of claim 1 and corresponding plural individual sample matrices; and simultaneously electro-eluting a charged molecule from each of said plural individual sample matrices into each of corresponding plural individual sample eluants.

Claim 6

Dependent Claim 14 words

The method of claim 4, further comprising simultaneously recovering said plural individual sample eluants.

Claim 7

Dependent Claim 73 words

An electro-elution apparatus comprising an anode wire comprising a plurality of serially connected conductive extensions, a cathode wire, and a plurality of sample chambers and a buffer reservoir, wherein each said anode wire conductive extension extends into a sample chamber and said cathode wire extends into said buffer reservoir, wherein each said sample chamber defines a flow path for flow of electrolytes between said cathode wire and each said anode wire conductive extension.

Claim 8

Dependent Claim 73 words

An electro-elution apparatus comprising an anode wire comprising a plurality of serially connected conductive extensions, a cathode wire, and a plurality of sample chambers and a buffer reservoir, wherein each said anode wire conductive extension extends into a sample chamber and said cathode wire extends into said buffer reservoir, wherein each said sample chamber defines a flow path for flow of electrolytes between said cathode wire and each said anode wire conductive extension.

Claim 9

Dependent Claim 37 words

The apparatus of claim 6 wherein said sample chamber comprises upper and lower portions, said lower portion comprising barrier means for allowing electrolyte flow but preventing liquid exchange between each said sample chamber and said buffer reservoir.

Claim 10

Dependent Claim 19 words

The apparatus of claim 7 wherein each said sample chamber defines an internal volume of less than 500 μl.

Claim 11

Dependent Claim 21 words

The apparatus of claim 7, further comprising a power supply to which said anode wire and said cathode wire electrically connect.

Claim 12

Dependent Claim 27 words

The apparatus of claim 7, further comprising insulated handle means for placing said anode wire such that said anode wire conductive extensions are extendable into sample chambers.

Claim 13

Dependent Claim 20 words

The apparatus of claim 10, said insulated handle means comprising a cover to which said anode wire is structurally attached.

Claim 14

Dependent Claim 35 words

The apparatus of claim 6, further comprising insulating means cooperative with said anode wire to insulate said anode wire between any two consecutive serially connected conductive extensions of said plurality of serially connected conductive extensions.

Claim 15

Independent Claim 56 words

A method of electro-elution, comprising electro-eluting a negatively charged molecule from each of plural individual sample matrices via corresponding plural electric fields comprising an electrified anode wire comprising a plurality of serially connected conductive extensions and an electrified cathode wire, wherein each said electric field extends from a single conductive anode extension and said cathode wire.

Claim 16

Independent Claim 56 words

A method of electro-elution, comprising electro-eluting a negatively charged molecule from each of plural individual sample matrices via corresponding plural electric fields comprising an electrified anode wire comprising a plurality of serially connected conductive extensions and an electrified cathode wire, wherein each said electric field extends from a single conductive anode extension and said cathode wire.

Claim 17

Independent Claim 84 words

A method of electro-elution, comprising the steps of (a) providing an apparatus comprising an anode wire comprising a plurality of serially connected conductive extensions and a cathode wire; (b) placing each of plural sample matrices containing a biological molecule between each said conductive anode extension and said cathode wire; and (c) providing electricity to said apparatus for a time and under buffer conditions sufficient to simultaneously elute said biological molecule from each said sample matrix to form a sample eluate containing said biological molecule.

Claim 18

Independent Claim 84 words

A method of electro-elution, comprising the steps of (a) providing an apparatus comprising an anode wire comprising a plurality of serially connected conductive extensions and a cathode wire; (b) placing each of plural sample matrices containing a biological molecule between each said conductive anode extension and said cathode wire; and (c) providing electricity to said apparatus for a time and under buffer conditions sufficient to simultaneously elute said biological molecule from each said sample matrix to form a sample eluate containing said biological molecule.

Claim 19

Dependent Claim 13 words

The method of claim 14, further comprising (d) collecting said plural sample eluates.

Claim 20

Dependent Claim 137 words

An electro-elution method comprising the steps of (a) providing an electro-elution apparatus comprising a cathode wire extending into a buffer reservoir, an anode wire comprising a plurality of serially connected conductive extensions, and a corresponding plurality of sample chambers, each sample chamber containing an eluant solution, wherein each said anode wire conductive extension is extendable into a said eluant solution and each said sample chamber defines a flow path for flow of electrolytes between said cathode wire in said buffer reservoir and each said anode wire conductive extensions; (b) placing each of a plurality of sample matrices containing a biological molecule to be eluted into each of said corresponding plurality of sample chambers; (c) providing sufficient electricity to said anode and cathode wires to elute said biological molecule from each said plural samples into said eluant solution.

Claim 21

Dependent Claim 137 words

An electro-elution method comprising the steps of (a) providing an electro-elution apparatus comprising a cathode wire extending into a buffer reservoir, an anode wire comprising a plurality of serially connected conductive extensions, and a corresponding plurality of sample chambers, each sample chamber containing an eluant solution, wherein each said anode wire conductive extension is extendable into a said eluant solution and each said sample chamber defines a flow path for flow of electrolytes between said cathode wire in said buffer reservoir and each said anode wire conductive extensions; (b) placing each of a plurality of sample matrices containing a biological molecule to be eluted into each of said corresponding plurality of sample chambers; (c) providing sufficient electricity to said anode and cathode wires to elute said biological molecule from each said plural samples into said eluant solution.

Claim 22

Dependent Claim 63 words

An electro-elution apparatus comprising first and second electrode wires, wherein said second wire comprises a plurality of serial conductive extensions, each said conductive extension being shaped so as to substantially conform to the internal shape of a sample chamber, wherein, upon electrification, said first and second wires are cooperable with a plurality of sample chambers simultaneously to define a plurality of electric fields.

Claim 23

Dependent Claim 63 words

An electro-elution apparatus comprising first and second electrode wires, wherein said second wire comprises a plurality of serial conductive extensions, each said conductive extension being shaped so as to substantially conform to the internal shape of a sample chamber, wherein, upon electrification, said first and second wires are cooperable with a plurality of sample chambers simultaneously to define a plurality of electric fields.

Claim 24

Dependent Claim 88 words

An electro-elution apparatus comprising (a) first and second electrode wires, each said wire including a plurality of conductive extensions, wherein said first and second wires cooperate to form a plurality of pairs of conductive extensions, each pair comprising a first wire conductive extension and a corresponding second wire conductive extension spaced apart so as to define a flowpath in conductive medium for the flow of ions; and (b) a plurality of sample chambers, wherein the members of each said pair of conductive extensions extend into a sample chamber.

Claim 25

Dependent Claim 88 words

An electro-elution apparatus comprising (a) first and second electrode wires, each said wire including a plurality of conductive extensions, wherein said first and second wires cooperate to form a plurality of pairs of conductive extensions, each pair comprising a first wire conductive extension and a corresponding second wire conductive extension spaced apart so as to define a flowpath in conductive medium for the flow of ions; and (b) a plurality of sample chambers, wherein the members of each said pair of conductive extensions extend into a sample chamber.

Claim 26

Dependent Claim 15 words

The apparatus of claim 17 wherein said first electrode wire further comprises plural conductive extensions.

Claim 27

Dependent Claim 26 words

The apparatus of claim 17 or 18 wherein said first or second electrode wires further comprises insulating material wrapped around the wire between said conductive extensions.

Claim 28

Dependent Claim 30 words

The apparatus of claim 18 wherein said second electrode wire is shaped such that each said second wire conductive extension conforms to the internal shape of each said sample chamber.

Claim 29

Dependent Claim 36 words

The apparatus of claim 17 or 18 wherein each said first electrode wire is shaped such that each said first wire conductive extension is extendable into a sample chamber without contacting said second wire conductive extension.

Claim 30

Dependent Claim 12 words

The apparatus of claim 17, further comprising a plurality of sample chambers.

Claim 31

Dependent Claim 30 words

The apparatus of claim 17 or 18, wherein at least one of said first and second electrode wires is insulatively adapted so as to allow manual movement of said wire.

Claim 32

Dependent Claim 20 words

An electro-elution kit comprising (a) the apparatus of claim 1, 2, 3, 6, or 18, and (b) packaging material therefore.

Claim 33

Independent Claim 143 words

A method of electro-eluting a biological molecule from a sample matrix, comprising (b) providing an electro-elution apparatus comprising first and second electrode wires electrically connected to an electric power source, wherein at least said second electrode wire includes plural conductive extensions; (a) providing plural sample chambers, each said chamber containing a sample matrix comprising said biological molecule and electro-elution buffer solution; (c) placing said first and second wires such that each said conductive extension of said second wire extends into a sample chamber of said plural sample chambers, said first and second wires being spaced apart so as to define a flow path for ions upon electrification; and (d) electro-eluting said biological molecule by imposing an electric field within each said sample chamber between said first and second wires sufficient to elute said biological molecule from said sample matrix into said buffer solution.

Claim 34

Independent Claim 143 words

A method of electro-eluting a biological molecule from a sample matrix, comprising (b) providing an electro-elution apparatus comprising first and second electrode wires electrically connected to an electric power source, wherein at least said second electrode wire includes plural conductive extensions; (a) providing plural sample chambers, each said chamber containing a sample matrix comprising said biological molecule and electro-elution buffer solution; (c) placing said first and second wires such that each said conductive extension of said second wire extends into a sample chamber of said plural sample chambers, said first and second wires being spaced apart so as to define a flow path for ions upon electrification; and (d) electro-eluting said biological molecule by imposing an electric field within each said sample chamber between said first and second wires sufficient to elute said biological molecule from said sample matrix into said buffer solution.

Claim 35

Dependent Claim 102 words

A method of electro-eluting a biological molecule from a sample matrix, comprising the steps of: (a) providing the apparatus of claim 18 and a plurality of sample chambers, each said chamber containing a sample matrix comprising a biological molecule and a conductive buffer solution, wherein each said conductive extension of said second electrode wire is immersed in said solution within said sample chamber; (b) electro-eluting said biological molecule from said sample matrix by imposing an electric field within each said sample chamber between said first and second electrode wires sufficient to elute said biological molecule from said sample matrix into said buffer.

Claim 36

Dependent Claim 81 words

A method of electro-eluting a biological molecule from a sample matrix, comprising the steps of: (a) providing the apparatus of claim 18, wherein each said sample chamber further comprises a sample matrix containing said biological molecule and conductive medium; (b) electro-eluting said biological molecule from said sample matrix by imposing an electric field within each said sample chamber between said first and second electrode wires sufficient to elute said biological molecule along said flowpath from said sample matrix into said buffer.

Claim 37

Independent Claim 27 words

The method of any of claims 26, 27, or 28 wherein said eluted biological molecule is selected from the group consisting of nucleic acid, protein, and carbohydrate.

Claim 38

Independent Claim 28 words

The method of any of claims 26, 27, or 28 wherein each said sample chamber provided in step (a) has an internal volume of less than 500 μl.

Claim 39

Dependent Claim 23 words

The method of claim 30 wherein each said sample chamber provided in step (a) has an internal volume of less than 250 μl.

Claim 40

Dependent Claim 23 words

The method of claim 31 wherein each said sample chamber provided in step (a) has an internal volume of less than 100 μl.

Claim 41

Independent Claim 25 words

The method of any of claims 26, 27, or 28 wherein said eluted biological molecule in each said sample chamber comprises less than 50 μg.

Claim 42

Dependent Claim 24 words

The method of claim 39 wherein said biological molecule eluted in said electro-eluting step in each said sample chamber comprises less than 10 μg.

Claim 43

Dependent Claim 23 words

The method of claim 34 wherein said biological molecule eluted in step (d) in each said sample chamber comprises less than 2 μg.

Annuity Stage	Window Opens	Payment Deadline	Surcharge Period Until	Estimated Fee	Status
3.5 Years (Year 4)	1998-05-15	1998-11-14	1999-05-16	$2000.00	Window Closed / Lapsed
7.5 Years (Year 8)	2002-05-15	2002-11-14	2003-05-16	$3760.00	Window Closed / Lapsed
11.5 Years (Year 12)	2006-05-15	2006-11-14	2007-05-16	$7700.00	Window Closed / Lapsed

Method and apparatus for electro-elution of biological molecules

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