US5397770A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Yeast-derived epidermal growth factor/urogastrone-like products
Assignee: Individual
•
Filing: 1993-02-23
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Granted: 1995-03-14
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Expires: 2013-02-23
Abstract
Abstract A medicinal composition comprising an Epidermal Growth Factor-like protein derived from yeast, and a carrier which is pharmaceutically acceptable and suitable for the type of administration and treatment for which the composition is to be used. The yeast-derived protein Has a molecular weight of about 4770 daltons and a murine Epidermal Growth Factor (mEGF) activity equivalent to at least 100 nanograms of mEGF by Elisa Assay Titration. The medicinal composition may be applied topically for purposes such as the treatment of external body wounds, or in ophthalmic surgery such as in cataract or corneal surgery, by incorporating a carrier therein which is pharmaceutically acceptable for the particular topical use. The composition may be administered orally for treatment of such ailments as peptic ulcers by incorporating therein a carrier which is pharmaceutically acceptable for oral administration in oral dosage form. Additionally, the present composition may be formulated in a suitable carrier for parenteral injection to promote bone and tendon repair in a warm blooded animal.
Inventors
- 👤 Robert H. Levin
- 👤 Stephen J. Keller
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 23:40
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/37; C07K14/39; C07K14/395; C07K14/435; C07K14/475; C07K14/485; A; A61; A61K; A61K38/00
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
159 words
A medicinal composition comprising, in admixture with a pharmaceutically acceptable carrier, a water-soluble yeast protein composition which is free of water-insoluble yeast components; which is insoluble in trichloroacetic acid, choloroform, absolute methanol, 95% ethanol, acetone, hexanes, petroleum ether, and 50% methanol-25% ether; which is soluble in acidic solutions containing 50% methanol, ethanol or acetone; which stimulates the growth and respiration of A431 cells in a culture without serum supplements; which has a murine Epidermal Growth Factor (mEGF) activity per milligram equivalent to at least 1000 nanograms of mEGF by Elisa Assay Titration, which EGF activity is provided by the presence therein of a water-soluble yeast protein having a molecular weight of about 4770 and approximately the following amino acid composition: ______________________________________
Asx (7) Thr (3) Ile (1)
Glx (5) Ala (3) Leu (2)
Ser (3) Pro (2) Phe (1)
Gly (5) Tyr (1) Lys (4)
His (1) Val (2) Trp (2), and
Arg (1) Met (o) Cys (6).
______________________________________
Claim 2
Dependent Claim
159 words
A medicinal composition comprising, in admixture with a pharmaceutically acceptable carrier, a water-soluble yeast protein composition which is free of water-insoluble yeast components; which is insoluble in trichloroacetic acid, choloroform, absolute methanol, 95% ethanol, acetone, hexanes, petroleum ether, and 50% methanol-25% ether; which is soluble in acidic solutions containing 50% methanol, ethanol or acetone; which stimulates the growth and respiration of A431 cells in a culture without serum supplements; which has a murine Epidermal Growth Factor (mEGF) activity per milligram equivalent to at least 1000 nanograms of mEGF by Elisa Assay Titration, which EGF activity is provided by the presence therein of a water-soluble yeast protein having a molecular weight of about 4770 and approximately the following amino acid composition: ______________________________________
Asx (7) Thr (3) Ile (1)
Glx (5) Ala (3) Leu (2)
Ser (3) Pro (2) Phe (1)
Gly (5) Tyr (1) Lys (4)
His (1) Val (2) Trp (2), and
Arg (1) Met (o) Cys (6).
______________________________________
Claim 3
Dependent Claim
11 words
A medicinal composition according to claim 1, in oral dosage form.
Claim 4
Dependent Claim
26 words
A medicinal composition according to claim 1, wherein said yeast protein composition has a per milligram mEGF activity at least equivalent to 3000 nanograms of mEGF.
Claim 5
Dependent Claim
11 words
A medicinal composition according to claim 3, in oral dosage form.
Claim 6
Dependent Claim
26 words
A medicinal composition according to claim 1, wherein the yeast protein composition has a per milligram mEGF activity at least equivalent to 114,000 nanograms of mEGF.
Claim 7
Dependent Claim
11 words
A medicinal composition according to claim 5, in oral dosage form.
Claim 8
Dependent Claim
26 words
A medicinal composition according to claim 1, wherein the yeast proteic composition has a per milligram mEGF activity at least equivalent to 214,000 nanograms of mEGF.
Claim 9
Dependent Claim
11 words
A medicinal composition according to claim 7, in oral dosage form.
Claim 10
Dependent Claim
26 words
A medicinal composition according to claim 1, wherein the yeast protein composition has a per milligram mEGF activity at least equivalent to 943,000 nanograms of mEGF.
Claim 11
Dependent Claim
11 words
A medicinal composition according to claim 9, in oral dosage form.
Claim 12
Dependent Claim
8 words
A topical medicinal composition according to claim 9.
Claim 13
Dependent Claim
8 words
An opthalmic medicinal composition according to claim 9.
Claim 14
Dependent Claim
8 words
A parenteral medicinal composition according to claim 9.
Claim 15
Dependent Claim
8 words
A topical medicinal composition according to claim 1.
Claim 16
Dependent Claim
8 words
An opthalmic medicinal composition according to claim 1.
Claim 17
Dependent Claim
8 words
A parenteral medicinal composition according to claim 1.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-03-13 | 1998-09-12 | 1999-03-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-03-13 | 2002-09-12 | 2003-03-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-03-13 | 2006-09-12 | 2007-03-14 | $7700.00 | Window Closed / Lapsed |