US5382425A
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Recombinant swinepox virus
Assignee: Syntro Corp
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Filing: 1992-01-13
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Granted: 1995-01-17
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Expires: 2012-01-13
Abstract
Abstract The present invention relates to a recombinant swinepox virus capable of replication comprising foreign DNA inserted into a site in the swinepox viral DNA which is not essential for replication of the swinepox virus. The invention further relates to homology vectors which produce recombinant swinepox viruses by inserting foreign DNA into swinepox viral DNA.
Inventors
- 👤 Mark D. Cochran
- 👤 David E. Junker
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 19:00
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/86; A; A61; A61P; A61P31/00; A61P31/12; C07; C07K; C07K14/00; C07K14/005; C07K14/195; C12N2710/00; C12N2710/00011; C12N2710/16011; C12N2710/16611; C12N2710/16622; C12N2710/16711; C12N2710/16722; C12N2710/24011; C12N2710/24111; C12N2710/24141; C12N2710/24143; C12N2720/00; C12N2720/00011; C12N2720/12011; C12N2720/12311; C12N2720/12322; C12N2750/00; C12N2750/00011; C12N2750/14011; C12N2750/14311; C12N2750/14322; C12N2760/00; C12N2760/00011; C12N2760/18011; C12N2760/18111; C12N2760/18122; C12N2770/00; C12N2770/00011; C12N2770/20011; C12N2770/20022; C12N2770/24011; C12N2770/24022
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
52 words
A recombinant swinepox virus which comprises a foreign DNA inserted within the swinepox virus genomic DNA, wherein the foreign DNA sequence is inserted within the larger HindIII to BglII subfragment of the HindIII M fragment of the genomic DNA, and is capable of being expressed in a swinepox virus infected hose cell.
Claim 2
Dependent Claim
52 words
A recombinant swinepox virus which comprises a foreign DNA inserted within the swinepox virus genomic DNA, wherein the foreign DNA sequence is inserted within the larger HindIII to BglII subfragment of the HindIII M fragment of the genomic DNA, and is capable of being expressed in a swinepox virus infected hose cell.
Claim 3
Dependent Claim
41 words
The recombinant swinepox virus of claim 1, wherein the insertion site is within an open reading frame contained within the HindIII to BglII subfragment, wherein the open reading frame contains the unique AccI site present in the HindIII to BglII subfragment.
Claim 4
Dependent Claim
24 words
The recombinant swinepox virus of claim 2, wherein the insertion size is the AccI restriction endonuclease site located in the HindIII to BglII subfragment.
Claim 5
Dependent Claim
26 words
The recombinant swinepox virus of claim 1, wherein the recombinant swinepox virus further comprises a foreign DNA sequence inserted within the swinepox virus thymidine kinase gene.
Claim 6
Dependent Claim
16 words
The recombinant swinepox virus of claim 1, wherein the foreign DNA sequence encodes a detectable marker.
Claim 7
Dependent Claim
15 words
The recombinant swinepox virus of claim 5, wherein the detectable marker is E. coli β-galactosidase.
Claim 8
Dependent Claim
14 words
The recombinant swinepox virus of claim 6, designated S-SPV-003 (ATCC Accession No. VR 2335).
Claim 9
Dependent Claim
16 words
The recombinant swinepox virus of claim 1, wherein the foreign DNA sequence encodes an antigenic polypeptide.
Claim 10
Dependent Claim
61 words
The recombinant swinepox virus of claim 8, wherein the antigenic polypeptide is selected from the group consisting essentially of PRV glycoprotein 50, PRV glycoprotein II, PRV glycoprotein III, PRV glycoprotein H, TGE glycoprotein 195, TGE matrix protein,, swine rotavirus glycoprotein 38, swine parvovirus capsid protein, Serpulina hyodysenteriae protective antigen, BVDV glycoprotein 55, NDV hemagglutinin-neuraminidase, swine flu hemagglutinin and swine flu neuraminidase.
Claim 11
Dependent Claim
15 words
The recombinant swinepox virus of claim 9, wherein the antigenic polypeptide is PRV glycoprotein 50.
Claim 12
Dependent Claim
14 words
The recombinant swinepox virus of claim 10, designated S-SPV-008 (ATCC Accession No. VR 2339).
Claim 13
Dependent Claim
63 words
A homology vector for producing a recombinant swinepox virus by inserting a foreign DNA sequence into the genomic DNA of a swinepox virus which comprises a foreign DNA sequence, wherein the foreign DNA sequence is controlled by a promoter and flanked by a nucleotide sequence homologous to the swinepox virus genomic DNA present within the HindIII to BglII subfragment of HindIII M fragment.
Claim 14
Dependent Claim
63 words
A homology vector for producing a recombinant swinepox virus by inserting a foreign DNA sequence into the genomic DNA of a swinepox virus which comprises a foreign DNA sequence, wherein the foreign DNA sequence is controlled by a promoter and flanked by a nucleotide sequence homologous to the swinepox virus genomic DNA present within the HindIII to BglII subfragment of HindIII M fragment.
Claim 15
Dependent Claim
37 words
The homology vector of claim 12, wherein the nucleotide sequence flanking the foreign DNA sequence is homologous to the swinepox virus genomic DNA present within the AccI restriction endonuclease site located in the HindIII to BglII subfragment.
Claim 16
Dependent Claim
15 words
The homology vector of claim 12, wherein the foreign DNA sequence encodes a detectable marker.
Claim 17
Dependent Claim
14 words
The homology vector of claim 13, wherein the detectable marker is E. coli β-galactosidase.
Claim 18
Dependent Claim
15 words
The homology vector of claim 12, wherein the foreign DNA sequence encodes an antigenic polypeptide.
Claim 19
Dependent Claim
15 words
The homology vector of claim 16, wherein the antigenic polypeptide is pseudorabies virus glycoprotein 50.
Claim 20
Dependent Claim
33 words
A recombinant swinepox virus of claim 8, wherein the antigenic polypeptide is or is from, Foot and Mouth Disease Virus, Hog Cholera Virus, Swine Influenza Virus, African Swine Fever Virus or Mycoplasma hyopneumoniae.
Claim 21
Dependent Claim
14 words
A recombinant swinepox virus of claim 8, designated S-SPV-009 (ATCC Accession No. VR 2344).
Claim 22
Dependent Claim
34 words
A homology vector of claim 16, wherein the antigenic polypeptide is or is from Serpulina hyodysenteriae, Foot and Mouth Disease Virus, Hog Cholera Virus, Swine Influenza Virus, African Swine Fever Virus or Mycoplasma hyopneumoniae.
References Cited (Backward Citations)
EP0261940A2
Cited
US5225336A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-01-16 | 1998-07-18 | 1999-01-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-01-16 | 2002-07-18 | 2003-01-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-01-16 | 2006-07-18 | 2007-01-17 | $7700.00 | Window Closed / Lapsed |