US5340453A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Analysis of carbohydrates
Assignee: Astromed Ltd
•
Filing: 1990-09-20
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Granted: 1994-08-23
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Expires: 2010-09-20
Abstract
Abstract Carbohydrate substances are separated or distinguished by a method which involves labelling carbohydrate substances with a labelling reagent comprising a fluorescent naphthalene ring structure having as a substituent a reactive group capable of reacting with a reducing sugar to bind thereto and also having at least one substituent group capable of carrying a charge but which does not react with reducing sugars and does not extinguish fluorescence of the labelling reagent; applying the labelled substances to an electrophoretic gel; and running the gel to cause differential migration of different substances. The preferred labelling reagents are aminoaphthalenesulphonic acids with one, two or three sulphonic acid groups, particularly 8-aminoaphthalene-1,3,6,-trisulphonic acid (ANTS), and 1-amino-4-naphthalene sulphonic acid (ANSA).
Inventors
- 👤 Peter Jackson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 20:19
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/66
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
90 words
A method of separating or distinguishing analyzing carbohydrate substance mixtures, comprising labelling carbohydrate substances with a labelling reagent comprising a fluorescent naphthalene ring structure having as a substituent a reactive group capable of reacting with a reducing sugar to bind thereto and also having at least one substituent group capable of carrying a charge but which does not react with reducing sugars and does not extinguish fluorescence of the labelling reagent; applying the labelled substances to an electrophoretic gel; and running the gel to cause differential migration of different substances.
Claim 2
Independent Claim
90 words
A method of separating or distinguishing analyzing carbohydrate substance mixtures, comprising labelling carbohydrate substances with a labelling reagent comprising a fluorescent naphthalene ring structure having as a substituent a reactive group capable of reacting with a reducing sugar to bind thereto and also having at least one substituent group capable of carrying a charge but which does not react with reducing sugars and does not extinguish fluorescence of the labelling reagent; applying the labelled substances to an electrophoretic gel; and running the gel to cause differential migration of different substances.
Claim 3
Dependent Claim
14 words
A method according to claim 1, wherein the reactive group is an amino group.
Claim 4
Dependent Claim
19 words
A method according to claim 2, wherein the amino group is attached to an alpha carbon of the naphthalene.
Claim 5
Dependent Claim
19 words
A method according to claim 1, wherein the group capable of carrying a charge is a sulphonic acid group.
Claim 6
Dependent Claim
13 words
A method according to claim 4, wherein the labelling reagent is 8-aminonaphthalene-1,3,6-trisulphonic acid.
Claim 7
Dependent Claim
14 words
A method according to claim 4, wherein the labelling reagent is 1-amino-4-naphthalene sulphonic acid.
Claim 8
Dependent Claim
15 words
A method according to claim 4, wherein the labelling reagent is a 1-amino-naphthalene disulphonic acid.
Claim 9
Dependent Claim
24 words
A method according to claim 1, wherein the labelling reagent further comprises one or more non-reactive substituent groups attached to the napthalene ring structure.
Claim 10
Dependent Claim
24 words
A method according to claim 1, wherein the electrophoretic gel comprises a polyacrylamide gel having a concentration in the range of 15% to 60%.
Claim 11
Dependent Claim
18 words
A method according to claim 9, wherein the gel has a concentration in the range 20% to 40%.
Claim 12
Dependent Claim
17 words
A method according to claim 9 or 10, wherein the gel is cross linked with N,N' methylebenbisacrylamide.
Claim 13
Independent Claim
20 words
A method according to any one of the preceding claims, wherein electrophoresis is carried out using a stacking buffer system.
Claim 14
Dependent Claim
18 words
A method according to claim 1, wherein the labelled carbohydrate substances are imaged with a charge coupled device.
Claim 15
Dependent Claim
20 words
A method according to claim 1, wherein the carbohydrate substances are derived from glycoproteins, proteoglycans, glycolipids, glycosphingolipids, polysaccharides, and glycosaminoglycans.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-08-22 | 1998-02-21 | 1998-08-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-08-22 | 2002-02-21 | 2002-08-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-08-22 | 2006-02-21 | 2006-08-23 | $7700.00 | Window Closed / Lapsed |