Patent US3273562A successfully refreshed from Google Patents.
US5334761A
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Cationic lipids
Assignee: Life Technologies Corp
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Filing: 1992-08-28
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Granted: 1994-08-02
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Expires: 2012-08-28
Abstract
Abstract The present invention discloses cationic lipids useful for making lipid aggregates for delivery of macromolecules and other compounds into cells. They are especially useful for the DNA-dependent transformation of cells. Also disclosed are lipids useful both for the delivery of macromolecules and also useful as intermediates for making other such lipids.
Inventors
- 👤 Gulilat Gebeyehu
- 👤 Joel A. Jessee
- 👤 Valentina C. Ciccarone
- 👤 Pamela Hawley-Nelson
- 👤 Anna Chytil
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 17:45
CPC Classifications
C; C07; C07C; C07C237/00; C07C237/02; C07C237/04; C07C237/10; A; A61; A61K; A61K47/00; A61K47/50; A61K47/51; A61K47/54; A61K47/543; A61K9/00; A61K9/10; A61K9/127; A61K9/1271; A61K9/1272; C07C217/00; C07C217/02; C07C217/04; C07C217/28
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
196 words
A composition having the structure wherein R 1 and R 2 independently of another, are C 1-23 alkyl or alkenyl, or ##STR12## alkyl or alkenyl, Z 1 and Z 2 independently of one another, are H or unbranched alkyl C 1-6 , q is 1 to 6, X is selected from any of X 1 -X 8 , X 15 and X 16 where X 1 is --(CH 2 ) n Br, (CH 2 ) n Cl, (CH 2 ) n F, (CH 2 ) n F or (CH 2 ) n I where n=0-6 or X 2 is --(CH 2 ) n NH 2 n=0-6 or X 3 is --NH--(CH 2 ) m --NH 2 m=2-6 or X 4 is --NH--(CH 2 ) 3 --NH--(CH 2 ) 4 --NH 2 or X 5 is --NH--(CH 2 ) 3 --NH--(CH 2 ) 4 --NH(CH 2 ) 3 --NH 2 or X 6 is ##STR13## X 7 is ##STR14## X 8 is ##STR15## X 15 is --(CH 2 ) r --SH where r is 0-6 or X 16 is --(CH 2 ) s --S--S--(CH 2 ) t --NH 2 where s is 0-6 and t is 2-6.
Claim 2
Dependent Claim
196 words
A composition having the structure wherein R 1 and R 2 independently of another, are C 1-23 alkyl or alkenyl, or ##STR12## alkyl or alkenyl, Z 1 and Z 2 independently of one another, are H or unbranched alkyl C 1-6 , q is 1 to 6, X is selected from any of X 1 -X 8 , X 15 and X 16 where X 1 is --(CH 2 ) n Br, (CH 2 ) n Cl, (CH 2 ) n F, (CH 2 ) n F or (CH 2 ) n I where n=0-6 or X 2 is --(CH 2 ) n NH 2 n=0-6 or X 3 is --NH--(CH 2 ) m --NH 2 m=2-6 or X 4 is --NH--(CH 2 ) 3 --NH--(CH 2 ) 4 --NH 2 or X 5 is --NH--(CH 2 ) 3 --NH--(CH 2 ) 4 --NH(CH 2 ) 3 --NH 2 or X 6 is ##STR13## X 7 is ##STR14## X 8 is ##STR15## X 15 is --(CH 2 ) r --SH where r is 0-6 or X 16 is --(CH 2 ) s --S--S--(CH 2 ) t --NH 2 where s is 0-6 and t is 2-6.
Claim 3
Dependent Claim
12 words
A compound according to claim 1 wherein X is X 2 .
Claim 4
Dependent Claim
16 words
A compound according to claim 1 wherein X is X 2 , and n is 2.
Claim 5
Dependent Claim
30 words
A compound according to claim 1 wherein X is X 2 , n is 1, R 1 =R 2 is C 18 alkenyl and Z 1 =Z 2 is methyl.
Claim 6
Dependent Claim
12 words
A compound according to claim 1 wherein X is X 1 .
Claim 7
Dependent Claim
21 words
A compound according to claim 1 wherein X is X 1 is (CH 2 ) n Br where n is 1.
Claim 8
Dependent Claim
36 words
A compound according to claim 1 wherein X is X 1 is (CH 2 ) n Br where n is 1, R 1 =R 2 is C 18 alkenyl and Z 1 =Z 2 is methyl.
Claim 9
Dependent Claim
12 words
A compound according to claim 1 wherein X is X 3 .
Claim 10
Dependent Claim
19 words
A compound according to claim 1 wherein X is X 3 , q is 2, and m is 3.
Claim 11
Dependent Claim
33 words
A compound according to claim 1 wherein X is X 3 , q is 2, m is 3, Z 1 =Z 2 is methyl and R 1 =R 2 is C 18 alkenyl.
Claim 12
Dependent Claim
12 words
A compound according to claim 1 wherein X is X 5 .
Claim 13
Dependent Claim
15 words
A compound according to claim 1 wherein X is X 5 and q is 2.
Claim 14
Dependent Claim
30 words
A compound according to claim 1 wherein X is X 5 , q is 2, Z 1 =Z 2 is methyl, and R 1 =R 2 is C 18 alkenyl.
Claim 15
Dependent Claim
12 words
A compound according to claim 1 wherein X is X 6 .
Claim 16
Dependent Claim
15 words
A compound according to claim 1 wherein X is X 6 and q is 2.
Claim 17
Dependent Claim
30 words
A compound according to claim 1 wherein X is X 6 , q is 2, Z 1 =Z 2 is methyl and R 1 =R 2 is C 18 alkenyl.
Claim 18
Dependent Claim
9 words
A lipid aggregate comprising a compound of claim 1.
Claim 19
Dependent Claim
15 words
A kit for preparing a lipid aggregate comprising a cationic lipid according to claim 1.
References Cited (Backward Citations)
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