US5324508A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for decreasing the formation of scar tissue using a purified mammalian monokine product
Assignee: University of Missouri–St. Louis
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Filing: 1992-06-16
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Granted: 1994-06-28
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Expires: 2012-06-16
Abstract
Abstract Fibroblast growth in a healing wound, and the consequent formation of scar tissue, may be inhibited by application of a macrophage monokine product, the macrophage monokine having a molecular weight of no more than about 10,000 Dalton. The macrophage monokine appears to be generally effective across species of mammals, and also exhibits the characteristic of stimulating hair growth on skin cells to which it is applied.
Inventors
- 👤 Edward H. Adelstein
- 👤 Barbro A. L. Barrett
- 👤 William H. Thornton, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 14:24
CPC Classifications
A; A61; A61Q; A61Q7/00; A61K; A61K8/00; A61K8/18; A61K8/30; A61K8/64; C; C07; C07K; C07K14/00; C07K14/435; C07K14/52; A61K38/00
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
140 words
A method of decreasing the formation of scar tissue during the healing of an injury comprising applying a purified mammalian monokine product to the site of an injury in an effective dose to decrease the formation of scar tissue during the healing of said injury, said purified monokine being produced by the steps of: culturing mammalian macrophage cells in a cell culture media for a period of no more than about 72 hours while allowing the cells to adhere to a surface, to cause said cells to excrete monokine into said media; and separating said cells from said culture media; and removing from said culture media monokine components having a molecular weight above about 6,000 Dalton to obtain a solution of said purified monokine which is essentially free of said components having a molecular weight of above about 6,000 Dalton.
Claim 2
Independent Claim
140 words
A method of decreasing the formation of scar tissue during the healing of an injury comprising applying a purified mammalian monokine product to the site of an injury in an effective dose to decrease the formation of scar tissue during the healing of said injury, said purified monokine being produced by the steps of: culturing mammalian macrophage cells in a cell culture media for a period of no more than about 72 hours while allowing the cells to adhere to a surface, to cause said cells to excrete monokine into said media; and separating said cells from said culture media; and removing from said culture media monokine components having a molecular weight above about 6,000 Dalton to obtain a solution of said purified monokine which is essentially free of said components having a molecular weight of above about 6,000 Dalton.
Claim 3
Dependent Claim
15 words
The method of claim 1 in which said solution of purified monokine is essentially cell-free.
Claim 4
Dependent Claim
18 words
The method of claim 1 in which said monokine is produced by the culturing of human macrophage cells.
Claim 5
Dependent Claim
23 words
The method of claim 1 in which said monokine is purified by separating out impurities having molecular weights of above about 4,000 Dalton.
Claim 6
Dependent Claim
15 words
The method of claim 1 in which said macrophage cells are obtained by peritoneal lavage.
Claim 7
Dependent Claim
14 words
The method of claim 1 in which said cell culture media is serum free.
Claim 8
Dependent Claim
19 words
The method of claim 1 in which the purified monokine product is applied to a granuloma of a horse.
Claim 9
Dependent Claim
34 words
The method of claim 1 in which said monokine is produced by the culturing of human macrophage cells and is purified by separating out monokine impurities having molecular weights of above about 4,000 Dalton.
Claim 10
Dependent Claim
15 words
The method of claim 8 in which said solution of purified monokine is essentially cell-free.
Claim 11
Dependent Claim
15 words
The method of claim 9 in which said macrophage cells are obtained by peritoneal lavage.
Claim 12
Dependent Claim
14 words
The method of claim 10 in which said cell culture media is serum free.
References Cited (Backward Citations)
US4929442A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-06-27 | 1997-12-27 | 1998-06-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-06-27 | 2001-12-27 | 2002-06-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-06-27 | 2005-12-27 | 2006-06-28 | $7700.00 | Window Closed / Lapsed |