DNA encoding the human A1 adenosine receptor

Claim 2

Isolated DNA encoding an A 1 adenosine receptor selected from the group consisting of: (a) isolated human genomic DNA which encodes the human A 1 adenosine receptor given herein as SEQ ID NO:6 and which contains the DNA sequences given herein as SEQ ID NO:1 and SEQ ID NO:3; (b) isolated human genomic DNA which hybridizes to isolated DNA of (a) above under conditions represented by a wash stringency of 0.3M NaCl, 0.03M sodium citrate, and 0.1% SDS at 60° C., and which encodes a human A 1 adenosine receptor; and (c) isolated DNA differing from the isolated DNAs of (a) and (b) above in nucleotide sequence due to the degeneracy of the genetic code, and which encodes an A 1 adenosine receptor encoded by isolated DNA of (a) or (b) above.

Claim 3

A recombinant DNA comprising vector DNA and a DNA according to claim 1.

Claim 4

A recombinant DNA according to claim 2, wherein said vector DNA comprises a vector selected from the group consisting of plasmids, adenoviruses, and cytomegaloviruses.

Claim 5

A recombinant DNA according to claim 2, wherein said vector DNA comprises a baculovirus vector.

Claim 6

A host cell containing a recombinant DNA of claim 2 and capable of expressing the encoded protein.

Claim 7

A host cell according to claim 5, wherein said host cell is a mammalian cell.

Claim 8

A host cell according to claim 5, wherein said host cell is selected from the group consisting of baby hamster kidney cells, mouse cells, human embryo cells, and chinese hamster ovary cells.

Claim 9

A host cell according to claim 5, wherein said host cell is an insect cell.

Claim 10

Isolated DNA encoding an A 1 adenosine receptor selected from the group consisting of: (a) isolated DNA which encodes a human A 1 adenosine receptor comprising DNA having the sequence given herein as SEQ ID NO:5; (b) isolated DNA which hybridizes to isolated DNA of (a) above under conditions represented by a wash stringency of 0.3M NaCl, 0.03M sodium citrate, and 0.1% SDS at 60° C., and which encodes a human A 1 adenosine receptor; and (c) isolated DNA differing from the isolated DNAs of (a) and (b) above in nucleotide sequence due to the degeneracy of the genetic code, and which encodes a human A 1 adenosine receptor encoded by isolated DNA of (a) or (b) above.

Claim 11

Isolated DNA encoding an A 1 adenosine receptor selected from the group consisting of: (a) isolated DNA which encodes a human A 1 adenosine receptor comprising DNA having the sequence given herein as SEQ ID NO:5; (b) isolated DNA which hybridizes to isolated DNA of (a) above under conditions represented by a wash stringency of 0.3M NaCl, 0.03M sodium citrate, and 0.1% SDS at 60° C., and which encodes a human A 1 adenosine receptor; and (c) isolated DNA differing from the isolated DNAs of (a) and (b) above in nucleotide sequence due to the degeneracy of the genetic code, and which encodes a human A 1 adenosine receptor encoded by isolated DNA of (a) or (b) above.

Claim 12

A recombinant DNA comprising vector DNA and a DNA according to claim 9.

Claim 13

A recombinant DNA according to claim 10, wherein said vector DNA comprises a vector selected from the group consisting of plasmids, adenoviruses, and cytomegaloviruses.

Claim 14

A recombinant DNA according to claim 10, wherein said vector DNA comprises a baculovirus vector.

Claim 15

A host cell containing a recombinant DNA of claim 10 and capable of expressing the encoded protein.

Claim 16

A host cell according to claim 13, wherein said host cell is a mammalian cell.

Claim 17

A host cell according to claim 13, wherein said host cell is selected from the group consisting of baby hamster kidney cells, mouse cells, human embryo cells, and chinese hamster ovary cells.

Claim 18

A host cell according to claim 13, wherein said host cell is an insect cell.

Claim 19

An oligonucleotide capable of binding to an intron of the A 1 adenosine receptor gene, said oligonucleotide selected from the group consisting of: (a) (i) isolated DNA comprising the first intron of the human genomic DNA encoding the human A 1 adenosine receptor according to SEQ ID NO:6, (ii) isolated DNA comprising the second intron of the human genomic DNA encoding the human A 1 adenosine receptor according to SEQ ID NO:6, (iii) isolated DNA comprising the third intron of the human genomic DNA encoding the human A 1 adenosine receptor according to SEQ ID NO:6, (iv) isolated DNA comprising the fourth intron of the human genomic DNA encoding the human A 1 adenosine receptor according to SEQ ID NO:6, and (v) isolated DNA comprising the fifth intron of the human genomic DNA encoding the human A 1 adenosine receptor according to SEQ ID NO:6; and (b) isolated DNA which hybridizes to the isolated DNAs of (a) above under conditions represented by a wash stringency of 0.3M NaCl, 0.03M sodium citrate, and 0.1% SDS at 60° C.

Claim 20

An oligonucleotide capable of binding to an intron of the A 1 adenosine receptor gene, said oligonucleotide selected from the group consisting of: (a) (i) isolated DNA comprising the first intron of the human genomic DNA encoding the human A 1 adenosine receptor according to SEQ ID NO:6, (ii) isolated DNA comprising the second intron of the human genomic DNA encoding the human A 1 adenosine receptor according to SEQ ID NO:6, (iii) isolated DNA comprising the third intron of the human genomic DNA encoding the human A 1 adenosine receptor according to SEQ ID NO:6, (iv) isolated DNA comprising the fourth intron of the human genomic DNA encoding the human A 1 adenosine receptor according to SEQ ID NO:6, and (v) isolated DNA comprising the fifth intron of the human genomic DNA encoding the human A 1 adenosine receptor according to SEQ ID NO:6; and (b) isolated DNA which hybridizes to the isolated DNAs of (a) above under conditions represented by a wash stringency of 0.3M NaCl, 0.03M sodium citrate, and 0.1% SDS at 60° C.

Claim 21

An oligonucleotide according to claim 17 labelled with a detectable group.

Claim 22

A recombinant DNA comprising vector DNA and an oligonucleotide according to claim 17.

Claim 23

A recombinant DNA according to claim 19, wherein said DNA is capable of exchanging said oligonucleotide with a homologous nucleotide in a suitable cell by homologous recombination.

Claim 24

Isolated human genomic DNA which encodes the human A 1 adenosine receptor given herein as SEQ ID NO:6 and which contains the DNA sequences given herein as SEQ ID NO:1 and SEQ ID NO;3.

Claim 25

Isolated human genomic DNA which encodes the human A 1 adenosine receptor given herein as SEQ ID NO:6 and which contains the DNA sequences given herein as SEQ ID NO:1 and SEQ ID NO;3.

Claim 26

A recombinant DNA comprising vector DNA and DNA according to claim 21.

Claim 27

A recombinant DNA according to claim 22, wherein said vector DNA comprises a vector selected from the group consisting of plasmids, adenoviruses, and cytomegaloviruses.

Claim 28

A recombinant DNA according to claim 22, wherein said vector DNA comprises a baculovirus vector.

Claim 29

A host cell containing a recombinant DNA of claim 22 and capable of expressing the encoded protein.

Claim 30

Isolated DNA which encodes a human A 1 adenosine receptor having the sequence given herein as SEQ ID NO:5.

Claim 31

Isolated DNA which encodes a human A 1 adenosine receptor having the sequence given herein as SEQ ID NO:5.

Claim 32

A recombinant DNA comprising vector DNA and a DNA according to claim 26.

Claim 33

A recombinant DNA according to claim 27, wherein said vector DNA comprises a vector selected from the group consisting of plasmids, adenoviruses, and cytomegaloviruses.

Claim 34

A recombinant DNA according to claim 27, wherein said vector DNA comprises a baculovirus vector.

Claim 35

A host cell containing a recombinant DNA of claim 27 and capable of expressing the encoded protein.