US5318898A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Production of recombinant bone-inducing proteins
Assignee: Genetics Institute LLC
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Filing: 1991-04-02
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Granted: 1994-06-07
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Expires: 2011-04-02
Abstract
Abstract An improved process for producing recombinant bone-inducing protein of the BMP-2 family as described. A suitable mammalian host cell transformed with a DNA encoding the protein is cultured in an appropriate culture medium to which is added about 10 to 1000 μg/ml dextran sulfate. The presence of dextran sulfate in the medium results in an increased yield of recombinant bone-inducing protein.
Inventors
- 👤 David I. Israel
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 04:40
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/38; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/51
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
80 words
In a process for producing a recombinant bone-inducing protein of the BMP-2 family which comprises culturing in a suitable culture medium a mammalian host cell grown in suspension, said host cell being transformed with a DNA sequence encoding said protein as set forth in SEQ ID NO:1, and recovering the protein from said culture medium, the improvement for increasing the yield of said bone-inducing protein which comprises adding about 10 to about 1000 μμg/ml dextran sulfate to said culture medium.
Claim 2
Dependent Claim
80 words
In a process for producing a recombinant bone-inducing protein of the BMP-2 family which comprises culturing in a suitable culture medium a mammalian host cell grown in suspension, said host cell being transformed with a DNA sequence encoding said protein as set forth in SEQ ID NO:1, and recovering the protein from said culture medium, the improvement for increasing the yield of said bone-inducing protein which comprises adding about 10 to about 1000 μμg/ml dextran sulfate to said culture medium.
Claim 3
Dependent Claim
21 words
The process of claim 1, wherein said amount of dextran sulfate is in the range of about 10 to 20 μg/ml.
Claim 4
Dependent Claim
81 words
In a process for producing a recombinant bone-inducing protein of the BMP-2 family which comprises culturing in a suitable culture medium a mammalian host cell grown in a monolayer, said host cell being transformed with a DNA sequence encoding said protein as set forth in SEQ ID NO:1, and recovering the protein from said culture medium, the improvement for increasing the yield of said bone-inducing protein which comprises adding about 100 to about 1000 μg/ml dextran sulfate to said culture medium.
Claim 5
Dependent Claim
81 words
In a process for producing a recombinant bone-inducing protein of the BMP-2 family which comprises culturing in a suitable culture medium a mammalian host cell grown in a monolayer, said host cell being transformed with a DNA sequence encoding said protein as set forth in SEQ ID NO:1, and recovering the protein from said culture medium, the improvement for increasing the yield of said bone-inducing protein which comprises adding about 100 to about 1000 μg/ml dextran sulfate to said culture medium.
References Cited (Backward Citations)
US3407076A
Cited
US4994387A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-06-06 | 1997-12-06 | 1998-06-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-06-06 | 2001-12-06 | 2002-06-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-06-06 | 2005-12-06 | 2006-06-07 | $7700.00 | Window Closed / Lapsed |