US5315122A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Apparatus and method for fluorescent lifetime measurement
Assignee: Becton Dickinson and Co
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Filing: 1992-08-25
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Granted: 1994-05-24
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Expires: 2012-08-25
Abstract
Abstract A flow cytometer measures fluorescence lifetimes by the phase shift of a reference signal and an emission from a fluorescent particle or cell in a flow chamber in order to identify the particle or cell. An acoustic optic modulator modulates laser light with a sinusoidal wave of a predetermined frequency to excite particles or cells. Detectors respond to emissions of individual particles or cells in the form of an output signal pulse at the predetermined frequency. Signals representative of fluorescence emitted and reference beam signals is measured. The particle is identified by the phase shift.
Inventors
- 👤 Bertram G. Pinsky
- 👤 John J. Ladasky, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 00:32
CPC Classifications
G; G01; G01N; G01N15/00; G01N15/10; G01N15/14; G01N15/1434; G01N21/00; G01N21/62; G01N21/63; G01N21/64; G01N21/6408; G01N15/1456; G01N15/1459; G01N2015/1454
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
92 words
A method of identifying a particle for use in a flow cytometer, the method comprising the steps of: providing a beam of light; intensity modulating the beam of light; passing the particle through the beam of intensity modulated light; collecting light from the particle for use as a reference; collecting fluorescence emitted by the particle; generating a first signal in response to the light collected as a reference and a second signal in response to the fluorescence collected; and determining the difference in phase between the first signal and the second signal.
Claim 2
Independent Claim
92 words
A method of identifying a particle for use in a flow cytometer, the method comprising the steps of: providing a beam of light; intensity modulating the beam of light; passing the particle through the beam of intensity modulated light; collecting light from the particle for use as a reference; collecting fluorescence emitted by the particle; generating a first signal in response to the light collected as a reference and a second signal in response to the fluorescence collected; and determining the difference in phase between the first signal and the second signal.
Claim 3
Dependent Claim
19 words
The method of claim 1 further comprising the step of using the difference in phase to identify the article.
Claim 4
Dependent Claim
20 words
The method of claim 1 wherein the light collected for use as a reference is light scattered by the particle.
Claim 5
Dependent Claim
20 words
The method of claim 1 wherein the light collected for use as a reference is fluorescence emitted by the particle.
Claim 6
Dependent Claim
34 words
The method of claim 1 wherein the step of determining the difference in phase between the first signal and the second signal comprises the step of multiplying the first signal by the second signal.
Claim 7
Dependent Claim
16 words
The method of claim 1 further comprising the steps of filtering the first and second signals.
Claim 8
Dependent Claim
16 words
The method of claim 1 further comprising the steps of limiting the first and second signals.
Claim 9
Dependent Claim
20 words
The method of claim 1 wherein the step of intensity modulating is performed by means of a traveling wave modulator.
Claim 10
Dependent Claim
76 words
A flow cytometer comprising: means for producing a light beam; means for intensity modulating the light beam; means for passing a fluorescently tagged particle through the modulated light beam such that light is the particle emits fluorescence on passing through the light beam; first fluorescence detecting means for detecting the fluorescence and generating a fluorescence signal in response to the fluorescence; means for determining the phase shift between the fluorescence light and the modulated light beam.
Claim 11
Dependent Claim
76 words
A flow cytometer comprising: means for producing a light beam; means for intensity modulating the light beam; means for passing a fluorescently tagged particle through the modulated light beam such that light is the particle emits fluorescence on passing through the light beam; first fluorescence detecting means for detecting the fluorescence and generating a fluorescence signal in response to the fluorescence; means for determining the phase shift between the fluorescence light and the modulated light beam.
Claim 12
Dependent Claim
60 words
The flow cytometer of claim 9 further comprising means for detecting at least part of the scattered light and generating a scatter signal in response to the scattered light and wherein the means for determining the phase shift between the fluorescence and the modulated light beam comprises means for determining the phase shift between the scattered light and the fluorescence.
Claim 13
Dependent Claim
55 words
The flow cytometer of claim 9 further comprising: second fluorescence detecting means for detecting fluorescence emitted by the particle; and wherein the means for determining the phase shift comprises means for determining the phase shift between the fluorescence detected by the first fluorescence detecting means and the fluorescence detected by the second fluorescence detecting means.
Claim 14
Dependent Claim
25 words
The flow cytometer of claim 9 wherein the means for determining the phase shift between the fluorescence and the modulated light beam comprises a mixer.
Claim 15
Dependent Claim
13 words
The flow cytometer of claim 9 comprising means for filtering the fluorescence signal.
Claim 16
Dependent Claim
13 words
The flow cytometer of claim 9 comprising means for limiting the fluorescence signal.
Claim 17
Dependent Claim
16 words
The flow cytometer of claim 9 wherein the means for modulating is a traveling wave modulator.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-05-23 | 1997-11-22 | 1998-05-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-05-23 | 2001-11-22 | 2002-05-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-05-23 | 2005-11-22 | 2006-05-24 | $7700.00 | Window Closed / Lapsed |