US5312733A
Expired / Lapsed
US
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Developmental marker gene of CD4-CD8 thymocytes
Assignee: Research Development Foundation
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Filing: 1991-04-11
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Granted: 1994-05-17
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Expires: 2011-04-11
Abstract
Abstract The present invention provides novel DNA sequences, recombinant DNA (rDNA) molecules, processes for producing novel T-cell proteins expressed in T-cell development, the novel T-cell proteins in substantially pure form and antibodies which bind to the novel proteins. More particularly, it relates to novel DNA sequences expressed in appropriate hosts and the novel T-cell proteins produced in these hosts. The present invention also provides novel transmembrane proteins in substantially pure form, rDNA molecules encoding transmembrane proteins and processes for producing the novel transmembrane proteins. The DNA sequences and recombinant DNA molecules of this invention are characterized in that they are expressed by T lymphoma cells and have at least one of the following characteristics: (1) is expressed in normal thymus, activated spleen cells, or gut associated lymphoid tissue, (2) is expressed in ovarian tissue, normal liver and/or in a stage specific manner in embryonic development and (3) encode novel transmembrane proteins having multiple membrane spanning domains.
Inventors
- 👤 Carol L. MacLeod
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 18:47
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; C07K16/28; C07K16/30; A; A61; A61P; A61P37/00; C07K14/00; C07K14/435; C07K14/46; C07K14/705; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12P; C12P21/00; C07K2317/00; C07K2317/30; C07K2317/34
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
78 words
An expression vehicle which comprises a DNA sequence of a gene selected from the group consisting of 19.1 (seq. id. No. 1), 19.2 (seq. id. no. 2), 19.4 (seq. id. no. 3), 19.5 (seq. id. no. 4) and 20.5 (seq. id. no. 5) wherein said expression vehicle is capable of replication in a host which comprises, in operable linkage: a) an origin or replication; b) a promoter; and c) a DNA sequence coding for a T cell protein.
Claim 2
Dependent Claim
78 words
An expression vehicle which comprises a DNA sequence of a gene selected from the group consisting of 19.1 (seq. id. No. 1), 19.2 (seq. id. no. 2), 19.4 (seq. id. no. 3), 19.5 (seq. id. no. 4) and 20.5 (seq. id. no. 5) wherein said expression vehicle is capable of replication in a host which comprises, in operable linkage: a) an origin or replication; b) a promoter; and c) a DNA sequence coding for a T cell protein.
Claim 3
Dependent Claim
43 words
The expression vehicle of claim 1, wherein said expression vehicle is a plasmid capable of replication in a host which comprises, in operable linkage: a) an origin of replication; b) a promoter; and c) a DNA sequence coding for a T cell protein.
Claim 4
Dependent Claim
92 words
The expression vehicle of claim 1, wherein said expression vehicle is a phage or plasmid capable of replication in a prokaryotic host which comprises, in operable linkage: a) a prokaryotic origin of replication; b) a prokaryotic promoter; and c) a DNA sequence coding for a T lymphoma cell protein, wherein the protein is encoded by a gene selected from the group of cDNA clones consisting of 19.1 (seq. id. no. 1), 19.2 (seq. id. no. 2), 19.4 (seq. id. no. 3), 19.5 (seq. id. no. 4) and 20.5 (seq. id. no. 5).
Claim 5
Dependent Claim
86 words
A vector comprising a DNA sequence coding for a T lymphoma cell protein, wherein said T cell protein is encoded by a gene selected from the group consisting of 19.1 (seq. id. no. 1), 19.2 (seq. id. no. 2) 19.4 (seq. id. no. 3), 19.5 (seq. id. no. 4), and 20.5 (seq. id. 5) and said vector is capable of replication in a host which comprises, in operable linkage: a) an origin of replication; b) a promoter; and c) a DNA sequence coding for said gene.
Claim 6
Dependent Claim
86 words
A vector comprising a DNA sequence coding for a T lymphoma cell protein, wherein said T cell protein is encoded by a gene selected from the group consisting of 19.1 (seq. id. no. 1), 19.2 (seq. id. no. 2) 19.4 (seq. id. no. 3), 19.5 (seq. id. no. 4), and 20.5 (seq. id. 5) and said vector is capable of replication in a host which comprises, in operable linkage: a) an origin of replication; b) a promoter; and c) a DNA sequence coding for said gene.
Claim 7
Dependent Claim
22 words
The vector of claim 4 wherein said vector is isolated from the group consisting of a plasmid, a phage and a cosmid.
Claim 8
Dependent Claim
19 words
The vector of claim 5, wherein said vector is pT7T3-19.1 (seq. id. no. 1) having ATCC Accession number 68298.
Claim 9
Dependent Claim
19 words
The vector of claim 5, wherein said vector is pT7T3-19.2 (seq. id. no. 2) having ATCC Accession number 68299.
Claim 10
Dependent Claim
21 words
The vector of claim 5, wherein said vector is pT7T3-19.4 (seq. id. no. 3) having ATCC Accession numbers 68300 through 68302.
Claim 11
Dependent Claim
19 words
The vector of claim 5, wherein said vector is pT7T3-19.5 (seq. id. no. 4) having ATCC Accession number 68303.
Claim 12
Dependent Claim
19 words
The vector of claim 5, wherein said vector is pT7T3-20.5 (seq. id. no. 5) having ATCC Accession number 68305.
Claim 13
Dependent Claim
52 words
A host transformed with a recombinant DNA molecule, wherein said recombinant DNA molecule comprises a DNA sequence for a gene selected from the group consisting of 19.1 (seq. id. no. 1), 19.2 (seq. id. no. 2), 19.4 (seq. id. no. 3), 19.5 (seq. id. no. 4) and 20.5 (seq. id. no. 5).
Claim 14
Dependent Claim
52 words
A host transformed with a recombinant DNA molecule, wherein said recombinant DNA molecule comprises a DNA sequence for a gene selected from the group consisting of 19.1 (seq. id. no. 1), 19.2 (seq. id. no. 2), 19.4 (seq. id. no. 3), 19.5 (seq. id. no. 4) and 20.5 (seq. id. no. 5).
Claim 15
Dependent Claim
9 words
The host of claim 20, which is E. coli.
References Cited (Backward Citations)
No citation records indexed.
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