US5286716A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Inhibitor of the proliferation of endothelial cells
Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenschaften eV
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Filing: 1991-03-01
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Granted: 1994-02-15
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Expires: 2011-03-01
Abstract
Abstract Protein which acts as an inhibitor of the proliferation of endothelial ce which is obtainable from baby hamster kidney cells (ATCC CCL 10) and has a molecular weight between 60 and 100 kD in gel filtration under native conditions and is thermally stable at 60° C., as well as a process for its isolation and its use in the treatment of diseases.
Inventors
- 👤 Werner Risau
- 👤 Hannes Drexler
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 23:23
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/515; A; A61; A61P; A61P27/00; A61P27/02; A61P29/00; A61P3/00; A61P3/08; A61P35/00; A61P43/00; A61P9/00; A61P9/06; C07K14/46; C07K14/47; C07K14/4701; C07K14/4702; C07K14/4703
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
89 words
A protein purified from baby hamster kidney cells which has a molecular weight between 60 and 100 kD as determined by gel filtration under native conditions and is thermally stable at 60° C., wherein said protein inhibits the proliferation of endothelial cells and is obtained by a process comprising the steps of: a) concentrating a conditioned medium of embryonic cells by ultrafiltration, b) precipitating the protein using ammonium sulfate, and c) purifying the protein further using affinity chromatography on heparin sepharose, anion-exchange chromatography, Affi-gel chromatography and/or hydrophobic interaction chromatography.
Claim 2
Dependent Claim
89 words
A protein purified from baby hamster kidney cells which has a molecular weight between 60 and 100 kD as determined by gel filtration under native conditions and is thermally stable at 60° C., wherein said protein inhibits the proliferation of endothelial cells and is obtained by a process comprising the steps of: a) concentrating a conditioned medium of embryonic cells by ultrafiltration, b) precipitating the protein using ammonium sulfate, and c) purifying the protein further using affinity chromatography on heparin sepharose, anion-exchange chromatography, Affi-gel chromatography and/or hydrophobic interaction chromatography.
Claim 3
Dependent Claim
18 words
The protein according to claim 1 wherein the baby hamster kidney cells are from cell line ATCC CCL10.
Claim 4
Dependent Claim
24 words
The protein according to claim 1 wherein said protein retains about 70% of the original activity after treatment at 60° C. for 45 minutes.
Claim 5
Dependent Claim
14 words
The protein according to claim 1 wherein said protein does not bind to heparin-sepharose.
Claim 6
Dependent Claim
49 words
A protein purified from baby hamster kidney cells which has a molecular weight between 60 and 100 kD as determined by gel filtration under native conditions and is thermally stable at 60° C., wherein said protein inhibits the proliferation of endothelial cells and does not bind to heparin sepharose.
Claim 7
Dependent Claim
49 words
A protein purified from baby hamster kidney cells which has a molecular weight between 60 and 100 kD as determined by gel filtration under native conditions and is thermally stable at 60° C., wherein said protein inhibits the proliferation of endothelial cells and does not bind to heparin sepharose.
Claim 8
Dependent Claim
73 words
A process for isolating a protein purified from baby hamster kidney cells which has a molecular weight between 60 and 100 kD as determined by gel filtration under native conditions and is thermally stable at 60° C., wherein said protein inhibits the proliferation of endothelial cells, comprising the steps of: a) concentrating a conditioned medium of embryonic cells, b) precipitating the protein using ammonium sulfate, and c) purifying the protein further using chromatography.
Claim 9
Dependent Claim
73 words
A process for isolating a protein purified from baby hamster kidney cells which has a molecular weight between 60 and 100 kD as determined by gel filtration under native conditions and is thermally stable at 60° C., wherein said protein inhibits the proliferation of endothelial cells, comprising the steps of: a) concentrating a conditioned medium of embryonic cells, b) precipitating the protein using ammonium sulfate, and c) purifying the protein further using chromatography.
Claim 10
Dependent Claim
20 words
The process according to claim 6 wherein said embryonic cells are baby hamster kidney cells from cell line ATCC CCL10.
Claim 11
Dependent Claim
38 words
The process according to claim 6 wherein ultrafiltration is used in step a) with a membrane that has a nominal exclusion size of 10 kD and in step b) ammonium sulfate is added up to about 75% saturation.
Claim 12
Dependent Claim
16 words
The process according to claim 6 wherein said chromatography includes anion exchange and hydrophobic interaction chromatography.
Claim 13
Dependent Claim
19 words
The process according to claim 6 wherein said chromatography includes heparin affinity chromatography, gel filtration chromatography and/or Affi-gel chromatography.
Claim 14
Dependent Claim
52 words
A pharmaceutical composition comprising a protein purified from baby hamster kidney cells which has a molecular weight between 60 and 100 kD as determined by gel filtration under native conditions and is thermally stable at 60° C., wherein said protein inhibits the proliferation of endothelial cells, in combination with a suitable carrier.
Claim 15
Dependent Claim
52 words
A pharmaceutical composition comprising a protein purified from baby hamster kidney cells which has a molecular weight between 60 and 100 kD as determined by gel filtration under native conditions and is thermally stable at 60° C., wherein said protein inhibits the proliferation of endothelial cells, in combination with a suitable carrier.
References Cited (Backward Citations)
US4210719A
Cited
US4229531A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-02-14 | 1997-08-16 | 1998-02-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-02-14 | 2001-08-16 | 2002-02-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-02-14 | 2005-08-16 | 2006-02-15 | $7700.00 | Window Closed / Lapsed |