US5283079A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process to make magnetically responsive fluorescent polymer particles
Assignee: Baxter Healthcare Corp
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Filing: 1989-12-14
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Granted: 1994-02-01
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Expires: 2009-12-14
Abstract
Abstract This invention provides a novel process of producing magnetically responsive fluorescent polymer particles comprising polymeric core particles coated evenly with a layer of polymer containing magnetically responsive metal oxide. A wide variety of polymeric particles with sizes ranging from 1 to 100 microns can be used a core particles and transformed into magnetically responsive polymer particles. The surface of these magnetically responsive polymer particles can be coated further with another layer of functionalized polymer. These magnetically responsive fluorescent polymer particles can be used for passive or covalent coupling of biological material such as antigens, antibodies, enzymes or DNA/RNA hybridization and used as solid phase for various types of immunoassays, DNA/RNA hybridization probes assays, affinity purification, cell separation and other medical, diagnostic, and industrial applications.
Inventors
- 👤 Chao-Huei J. Wang
- 👤 Dinesh O. Shah
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 12:44
CPC Classifications
B; B01; B01J; B01J20/00; B01J20/28; B01J20/28002; B01J20/28004; A; A61; A61M; A61M1/00; A61M1/36; A61M1/3618; B01J20/28009; B01J20/28014; B01J20/28016; B01J8/00; B01J8/008; B03; B03C; B03C1/00; B03C1/005; B03C1/01; C; C08; C08F; C08F257/00; C08F257/02; C08F285/00; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816; C12Q1/6834; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; G01N33/54326; G01N33/5434; G01N33/58; G01N33/582; G01N33/585; H; H01; H01F; H01F1/00; H01F1/01; H01F1/03; H01F1/032; H01F1/10; H01F1/11; H01F1/111; G01N2446/00; G01N2446/10; G01N2446/40; G01N2446/80; G01N2446/84; G01N2446/86
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
49 words
A process to make monodispersed fluorescent magnetic particles of uniform size distribution and magnetic content comprising: a) evenly coating a fluorescent core polymer particle with a magnetically responsive metal oxide and polymer combination, said polymer combination being comprised of monomers able to absorb to said inner core polymer particle.
Claim 2
Dependent Claim
49 words
A process to make monodispersed fluorescent magnetic particles of uniform size distribution and magnetic content comprising: a) evenly coating a fluorescent core polymer particle with a magnetically responsive metal oxide and polymer combination, said polymer combination being comprised of monomers able to absorb to said inner core polymer particle.
Claim 3
Dependent Claim
22 words
The process of claim 1 wherein said fluorescent core polymer particle is comprised of polystyrene or cross-linked polystyrene, incorporated with fluorescent dye.
Claim 4
Dependent Claim
18 words
The process of claim 1 wherein said fluorescent core polymer particle ranges from about 1 to 100 microns.
Claim 5
Dependent Claim
17 words
The process of claim 1 wherein said magnetically responsive metal oxide is about 1 micron or less.
Claim 6
Dependent Claim
24 words
The process of claim 1 wherein said magnetically responsive metal oxide is selected from the group consisting of superparamagnetic, paramagnetic, or ferromagnetic metal oxide.
Claim 7
Dependent Claim
30 words
The process of claim 1 wherein the polymer of said magnetically responsive metal oxide and polymer combination is selected from the group consisting of polystyrene, cross-linked polystyrene or, functionalized polystyrene.
Claim 8
Dependent Claim
15 words
The process of claim 1 wherein the metal oxide is formed from transition metal salts.
Claim 9
Dependent Claim
25 words
The process of claim 1 wherein additional metal oxide and polymer combinations are added to evenly coat said first metal oxide and polymer combination coating.
Claim 10
Dependent Claim
76 words
A process to make monodispersed fluorescent magnetic particles of uniform size distribution and magnetic content comprising: a) evenly coating a core polymer particle with a magnetically responsive metal oxide and polymer combination, said polymer combination being comprised of monomers able to adsorb to said inner core polymer particle and said polymer combination containing a fluorescent dye or combination of fluorescent dyes; and b) coating said magnetically responsive metal oxide and polymer combination with an outer polymer.
Claim 11
Dependent Claim
76 words
A process to make monodispersed fluorescent magnetic particles of uniform size distribution and magnetic content comprising: a) evenly coating a core polymer particle with a magnetically responsive metal oxide and polymer combination, said polymer combination being comprised of monomers able to adsorb to said inner core polymer particle and said polymer combination containing a fluorescent dye or combination of fluorescent dyes; and b) coating said magnetically responsive metal oxide and polymer combination with an outer polymer.
Claim 12
Dependent Claim
19 words
The process of claim 9 wherein said core polymer particle is comprised of polystyrene, cross-linked polystyrene or functionalized polystyrene.
Claim 13
Dependent Claim
17 words
The process of claim 9 wherein said core polymer particle ranges from about 1 to 100 microns.
Claim 14
Dependent Claim
18 words
The process of claim 9 wherein said magnetically responsive metal oxide particle is about 1 micron or less.
Claim 15
Dependent Claim
24 words
The process of claim 9 wherein said magnetically responsive metal oxide is selected from the group consisting of superparamagnetic, paramagnetic, or ferromagnetic metal oxide.
Claim 16
Dependent Claim
30 words
The process of claim 9 wherein the polymer of said magnetically responsive metal oxide and polymer combination is selected from the group consisting of polystyrene, cross-linked polystyrene, or functionalized polystyrene.
Claim 17
Dependent Claim
15 words
The process of claim 9 wherein the metal oxide is formed from transition metal salts.
Claim 18
Dependent Claim
23 words
The process of claim 9 wherein said outer polymer coating is selected from the group consisting of polystyrene, cross-linked polystyrene, or functionalized polystyrene.
Claim 19
Dependent Claim
61 words
A process to make monodispersed fluorescent magnetic particles of uniform size distribution and magnetic content comprising: a) evenly coating a core polymer particle with a magnetically responsive metal oxide and polymer combination, said polymer combination being comprised of monomers able to adsorb to said inner core polymer particle and said polymer combination containing a fluorescent dye or combination of fluorescent dyes.
Claim 20
Dependent Claim
61 words
A process to make monodispersed fluorescent magnetic particles of uniform size distribution and magnetic content comprising: a) evenly coating a core polymer particle with a magnetically responsive metal oxide and polymer combination, said polymer combination being comprised of monomers able to adsorb to said inner core polymer particle and said polymer combination containing a fluorescent dye or combination of fluorescent dyes.
Claim 21
Dependent Claim
17 words
The process of claim 17 wherein said core polymer particle is comprised of polystyrene or cross-linked polystyrene.
Claim 22
Dependent Claim
17 words
The process of claim 17 wherein said core polymer particle ranges from about 1 to 100 microns.
Claim 23
Dependent Claim
18 words
The process of claim 17 wherein said magnetically responsive metal oxide particle is about 1 micron or less.
Claim 24
Dependent Claim
24 words
The process of claim 17 wherein said magnetically responsive metal oxide is selected from the group consisting of superparamagnetic, paramagnetic, or ferromagnetic metal oxide.
Claim 25
Dependent Claim
30 words
The process of claim 17 wherein the polymer of said magnetically responsive metal oxide and polymer combination is selected from the group consisting of polystyrene, cross-linked polystyrene, or functionalized polystyrene.
Claim 26
Dependent Claim
15 words
The process of claim 17 wherein the metal oxide is formed from transition metal salts.
Claim 27
Dependent Claim
22 words
The process of claim 17 wherein additional metal oxide and polymer combinations evenly coat said first metal oxide and polymer combination coating.
Claim 28
Dependent Claim
64 words
A process to make monodispersed fluorescent magnetic particles of uniform size distribution and magnetic content comprising: a) evenly coating a fluorescent core polymer particle with a magnetically responsive metal oxide and polymer combination, said polymer combination being comprised of monomers able to adsorb to said inner core polymer particle; and b) coating said magnetically responsive metal oxide and polymer combination with an outer polymer.
Claim 29
Dependent Claim
64 words
A process to make monodispersed fluorescent magnetic particles of uniform size distribution and magnetic content comprising: a) evenly coating a fluorescent core polymer particle with a magnetically responsive metal oxide and polymer combination, said polymer combination being comprised of monomers able to adsorb to said inner core polymer particle; and b) coating said magnetically responsive metal oxide and polymer combination with an outer polymer.
References Cited (Backward Citations)
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