US5279941A
Expired / Lapsed
US
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Determination of endometrial receptivity toward embryo implantation
Assignee: Cytyc Corp
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Filing: 1992-06-12
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Granted: 1994-01-18
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Expires: 2012-06-12
Abstract
Abstract Methods are provided for detecting receptivity of mammalian endometrium to embryo implantation comprising obtaining a sample of the endometrium, contacting the endometrium with a monoclonal antibody for (beta)3 integrin and detecting (beta)3 integrin in the endometrium. The invention also provides for methods of diagnosing infertility in a mammal and methods of detecting the window of embryo implantation in endometrium. Methods of in vitro fertilization, methods of preventing embryo implantation and a method of monitoring endometrial maturation are also within the scope of the present invention. The present invention is also directed to contraceptives. Diagnostic kits useful in the practice of the methods of the invention are also provided.
Inventors
- 👤 Bruce Lessey
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 01:37
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/566; G01N33/567; A; A61; A61D; A61D19/00; A61D19/04; A61K; A61K35/00; A61K35/12; A61K35/48; C; C07; C07K; C07K16/00; C07K16/18; C07K16/28; C07K16/2839; C07K16/2848; G01N33/575; G01N33/5755; G01N33/68; G01N33/6887; G01N33/6893; A61K38/00; G01N2333/00; G01N2333/435; G01N2333/705; G01N2333/70546; G01N2333/70557; G01N2474/00; G01N2474/20
Claims Analysis
Total Claims: 36
Claim 1
Independent Claim
54 words
A method for detecting receptivity of endometrium to embryo implantation comprising: a. obtaining a sample of said endometrium, b. contacting said sample with a monoclonal antibody specific for β 3 integrin, c. detecting the presence or absence of β 3 in said sample, and d. correlating the presence of β 3 with endometrial receptivity.
Claim 2
Independent Claim
54 words
A method for detecting receptivity of endometrium to embryo implantation comprising: a. obtaining a sample of said endometrium, b. contacting said sample with a monoclonal antibody specific for β 3 integrin, c. detecting the presence or absence of β 3 in said sample, and d. correlating the presence of β 3 with endometrial receptivity.
Claim 3
Dependent Claim
11 words
The method of claim 1 wherein said monoclonal antibody is SSA6.
Claim 4
Dependent Claim
15 words
The method of claim 1 wherein said β 3 is combined with another integrin subunit.
Claim 5
Dependent Claim
14 words
The method of claim 3 wherein said other integrin subunit is α v .
Claim 6
Dependent Claim
11 words
The method of claim 4 wherein said monoclonal antibody is 23C6.
Claim 7
Dependent Claim
46 words
The method of claim 1 wherein said sample is a tissue section and said detecting step comprises immunohistochemically staining said sample with a labeled secondary antibody which binds the anti-β 3 antibody, wherein the presence of said label corresponds to the presence of β 3 integrin.
Claim 8
Dependent Claim
54 words
The method of claim 1 wherein said sample is a cell extract and said contacting said detecting steps comprise immunoblotting with said monoclonal antibody specific for β 3 integrin and labeled secondary antibody which binds the anti-β 3 integrin antibody, wherein the presence of said label corresponds to the presence of β 3 integrin.
Claim 9
Dependent Claim
10 words
The method of claim 1 wherein said endometrium is human.
Claim 10
Independent Claim
70 words
A method for diagnosing infertility in a mammal comprising: a. obtaining a sample of endometrium from said mammal, b. contacting said sample with a monoclonal antibody specific for β 3 integrin, c. detecting the expression of β 3 in said sample, d. repeating steps a.-c. with samples of said endometrium at a plurality of stages of the menstrual cycle, and e. correlating the lack of β 3 expression with infertility.
Claim 11
Independent Claim
70 words
A method for diagnosing infertility in a mammal comprising: a. obtaining a sample of endometrium from said mammal, b. contacting said sample with a monoclonal antibody specific for β 3 integrin, c. detecting the expression of β 3 in said sample, d. repeating steps a.-c. with samples of said endometrium at a plurality of stages of the menstrual cycle, and e. correlating the lack of β 3 expression with infertility.
Claim 12
Dependent Claim
11 words
The method of claim 9 wherein said monoclonal antibody is SSA6.
Claim 13
Dependent Claim
15 words
The method of claim 9 wherein said β 3 is combined with another integrin subunit.
Claim 14
Dependent Claim
14 words
The method of claim 11 wherein said other integrin subunit is α v .
Claim 15
Dependent Claim
11 words
The method of claim 12 wherein said monoclonal antibody is 23C6.
Claim 16
Dependent Claim
10 words
The method of claim 9 wherein said mammal is human.
Claim 17
Dependent Claim
46 words
The method of claim 9 wherein said sample is a tissue section and said detecting step comprises immunohistochemically staining said sample with a labeled secondary antibody which binds the anti-β 3 antibody, wherein the presence of said label corresponds to the presence of β 3 integrin.
Claim 18
Dependent Claim
54 words
The method of claim 9 wherein said sample is a cell extract and said contacting and detecting steps comprise immunoblotting with said monoclonal antibody specific for β 3 integrin and labeled secondary antibody which binds the anti-β 3 integrin antibody, wherein the presence of said label corresponds to the presence of β 3 integrin.
Claim 19
Dependent Claim
24 words
The method of claim 9 wherein said stages of the menstrual cycle are selected from the group consisting of follicular phase and secretory phase.
Claim 20
Independent Claim
76 words
A method of detecting the window of embryo implantation in endometrium comprising: a. obtaining a sample of endometrium, b. contacting said sample of endometrium with a monoclonal antibody specific for β 3 integrin, c. detecting the presence or absence of β 3 in said sample, d. repeating steps a.-c. with samples of endometrium at a plurality of stages of the menstrual cycle, and e. correlating the presence of β 3 with the window of embryo implantation.
Claim 21
Independent Claim
76 words
A method of detecting the window of embryo implantation in endometrium comprising: a. obtaining a sample of endometrium, b. contacting said sample of endometrium with a monoclonal antibody specific for β 3 integrin, c. detecting the presence or absence of β 3 in said sample, d. repeating steps a.-c. with samples of endometrium at a plurality of stages of the menstrual cycle, and e. correlating the presence of β 3 with the window of embryo implantation.
Claim 22
Dependent Claim
11 words
The method of claim 18 wherein said monoclonal antibody is SSA6.
Claim 23
Dependent Claim
15 words
The method of claim 18 wherein said β 3 is combined with another integrin subunit.
Claim 24
Dependent Claim
14 words
The method of claim 20 wherein said other integrin subunit is α v .
Claim 25
Dependent Claim
11 words
The method of claim 21 wherein said monoclonal antibody is 23C6.
Claim 26
Dependent Claim
46 words
The method of claim 18 wherein said sample is a tissue section and said detecting step comprises immunohistochemically staining said sample with a labeled secondary antibody which binds the anti-β 3 antibody, wherein the presence of said label corresponds to the presence of β 3 integrin.
Claim 27
Dependent Claim
54 words
The method of claim 18 wherein said sample is a cell extract and said contacting and detecting steps comprise immunoblotting with said monoclonal antibody specific for β 3 integrin and labeled secondary antibody with binds the anti-β 3 integrin antibody, wherein the presence of said label corresponds to the presence of β 3 integrin.
Claim 28
Independent Claim
69 words
A method of monitoring endometrial maturation in a mammal comprising: a. obtaining a sample of the endometrium, b. contacting said sample with a monoclonal antibody specific for β 3 integrin, c. detecting the appearance of β 3 in said sample, d. repeating steps a.-c. with samples of endometrium at a plurality of stages of the endometrial cycle, and e. correlating the appearance of β 3 integrin with endometrial maturation.
Claim 29
Independent Claim
69 words
A method of monitoring endometrial maturation in a mammal comprising: a. obtaining a sample of the endometrium, b. contacting said sample with a monoclonal antibody specific for β 3 integrin, c. detecting the appearance of β 3 in said sample, d. repeating steps a.-c. with samples of endometrium at a plurality of stages of the endometrial cycle, and e. correlating the appearance of β 3 integrin with endometrial maturation.
Claim 30
Dependent Claim
11 words
The method of claim 25 wherein said monoclonal antibody is SSA6.
Claim 31
Dependent Claim
15 words
The method of claim 25 wherein said β 3 is combined with another integrin subunit.
Claim 32
Dependent Claim
14 words
The method of claim 27 wherein said other integrin subunit is α v .
Claim 33
Dependent Claim
11 words
The method of claim 28 wherein said monoclonal antibody is 23C6.
Claim 34
Dependent Claim
46 words
The method of claim 25 wherein said sample is a tissue section and said detecting step comprises immunohistochemically staining said sample with a labeled secondary antibody which binds the anti-β 3 antibody, wherein the presence of said label corresponds to the presence of β 3 integrin.
Claim 35
Dependent Claim
54 words
The method of claim 25 wherein said sample is a cell extract and said contacting and detecting steps comprise immunoblotting with said monoclonal antibody specific for β 3 integrin and labeled secondary antibody which binds the anti-β 3 integrin antibody, wherein the presence of said label corresponds to the presence of β 3 integrin.
Claim 36
Dependent Claim
24 words
The method of claim 25 wherein said stages of the endometrial cycle are selected from the group consisting of follicular phase and secretory phase.
References Cited (Backward Citations)
US5057604A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-01-17 | 1997-07-19 | 1998-01-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-01-17 | 2001-07-19 | 2002-01-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-01-17 | 2005-07-19 | 2006-01-18 | $7700.00 | Window Closed / Lapsed |