US5279833A
Expired / Lapsed
US
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Liposomal transfection of nucleic acids into animal cells
Assignee: Yale University
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Filing: 1990-04-04
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Granted: 1994-01-18
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Expires: 2010-04-04
Abstract
Abstract A reagent for transfecting a nucleic acid into an animal cell containing a neutral phospholipid such as dioleoylphosphatidylethanolamine, a cationic lipid such as stearylamine or dimethyldioctadecylammonium bromide, a tertiary carbyl amine, or a benzethonium salt such as methylbenzethonium chloride. A method for using this reagent in the transfection process is also described.
Inventors
- 👤 John K. Rose
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 16:28
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/87; C12N15/88; A; A61; A61K; A61K9/00; A61K9/10; A61K9/127; A61K9/1271; A61K9/1272
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
81 words
A liposome for introducing a nucleic acid into an animal cell comprising (a) a neutral lipid selected from the group consisting of lecithin, lysolecithin, lysophosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, sphinogomyelin, cephalin, cardiolipin, phosphatidic acid, cerebrosides, dioleoylphosphatidylcholine, dipalmitoylphosphatidylcholine, dioleoylphosphatidylglycerol, dipalmitoylphosphatidylglycerol, palmitoyloleoylphosphatidylcholine, palmitoyloleoylphosphatidylethanolamine, diheptadecanoylphosphatidylethanolamine, dilauroylphosphatidylethanolamine, dimyristoylphosphatidylethanolamine, distearoylphosphatidylethanolamine, beta-linoleoyl-gamma-palmitoylphosphatidylethanolamine and beta-oleoyl-gamma-palmitoylphosphatidylethanolamine, and (b) a cationic lipid selection from the group consisting of dimethyldioctadecylammonium bromide, cetylidimethylethylammonium bromide, stearylamine and methylbenzethonium chloride, wherein the weight ratio of the cationic lipid to the neutral lipid is 6:30 to 12:30.
Claim 2
Dependent Claim
81 words
A liposome for introducing a nucleic acid into an animal cell comprising (a) a neutral lipid selected from the group consisting of lecithin, lysolecithin, lysophosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, sphinogomyelin, cephalin, cardiolipin, phosphatidic acid, cerebrosides, dioleoylphosphatidylcholine, dipalmitoylphosphatidylcholine, dioleoylphosphatidylglycerol, dipalmitoylphosphatidylglycerol, palmitoyloleoylphosphatidylcholine, palmitoyloleoylphosphatidylethanolamine, diheptadecanoylphosphatidylethanolamine, dilauroylphosphatidylethanolamine, dimyristoylphosphatidylethanolamine, distearoylphosphatidylethanolamine, beta-linoleoyl-gamma-palmitoylphosphatidylethanolamine and beta-oleoyl-gamma-palmitoylphosphatidylethanolamine, and (b) a cationic lipid selection from the group consisting of dimethyldioctadecylammonium bromide, cetylidimethylethylammonium bromide, stearylamine and methylbenzethonium chloride, wherein the weight ratio of the cationic lipid to the neutral lipid is 6:30 to 12:30.
Claim 3
Dependent Claim
11 words
The liposome of claim 1, wherein the neutral lipid is dioleoylphosphatidylethanolamine.
Claim 4
Dependent Claim
12 words
The liposome of claim 1, wherein the cationic lipid is cetyldimethylethylammonium bromide.
Claim 5
Dependent Claim
12 words
The liposome of claim 1, wherein the cationic lipid is dimethyldioctadecylammonium bromide.
Claim 6
Dependent Claim
11 words
The liposome of claim 1, wherein the cationic lipid is stearylamine.
Claim 7
Dependent Claim
12 words
The liposome of claim 1, wherein the cationic lipid is methylbenzothonium chloride.
Claim 8
Dependent Claim
41 words
A composition comprising the liposome of claim 1, a nucleic acid, and an animal cell selected from the group consisting of arythrocytes, baby hamster kidney cells, AtI 20 cells, RK-13 cells, HeLa cells, Madin-Darby canine kidney cells and monkey COS cells.
Claim 9
Dependent Claim
37 words
A method for introducing RNA or DNA into an animal cell comprising (a) contacting said RNA or DNA with the liposome of claim 1 and then (b) contacting said liposome-RNA or liposome-DNA complex with an animal cell.
Claim 10
Dependent Claim
24 words
The method according to claim 8, wherein the method is carried out at a temperature of 37° C. for a period of three hours.
Claim 11
Dependent Claim
37 words
The method according to claim 8, wherein the animal cell is selected from the group consisting of arythrocytes, baby hamster kidney cells, AtI 20 cells, RK-13 cells, Heln cells, Madin-Darby canine kidney cells and monkey COS cells.
Claim 12
Dependent Claim
37 words
The method according to claim 9, wherein the animal cell is selected from the group consisting of erythrocytes, baby hamster kidney cells, AtI 20 cells, RK-13 cells, Heln cells, Madin-Darby canine kidney cells and monkey COS cells.
References Cited (Backward Citations)
US4897355A
Cited
US4946787A
Cited
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