US5278046A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Soybean peroxidase assays
Assignee: Wiley Organics Inc
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Filing: 1991-09-16
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Granted: 1994-01-11
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Expires: 2011-09-16
Abstract
Abstract An analytical composition comprising antibody conjugate, an indicator which is capable of providing a detectable condition in the presence of peroxidase and hydrogen peroxide assays performed using this composition are described.
Inventors
- 👤 Mark A. Johnson
- 👤 Alexander R. Pokora
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 14:25
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/535; C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/97; Y10S435/975
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
29 words
An analytical composition comprising soybean peroxidase or a soybean peroxidase-antibody conjugate, an indicator which is capable of providing a detectable condition in the presence of peroxidase and hydrogen peroxide.
Claim 2
Dependent Claim
29 words
An analytical composition comprising soybean peroxidase or a soybean peroxidase-antibody conjugate, an indicator which is capable of providing a detectable condition in the presence of peroxidase and hydrogen peroxide.
Claim 3
Dependent Claim
12 words
The composition of claim 1 wherein said indicator is a leuco dye.
Claim 4
Dependent Claim
14 words
The composition of claim 1 wherein said composition comprises soybean peroxidase and said indicator.
Claim 5
Dependent Claim
16 words
The composition of claim 1 wherein said composition comprises a soybean peroxidase-antibody conjugate and said indicator.
Claim 6
Dependent Claim
17 words
An analytical element comprising an adsorbent carrier material containing soybean peroxidase or a soybean peroxidase antibody conjugate.
Claim 7
Dependent Claim
17 words
An analytical element comprising an adsorbent carrier material containing soybean peroxidase or a soybean peroxidase antibody conjugate.
Claim 8
Independent Claim
63 words
A method for the determination of an analyte comprising the steps of contacting a sample of a liquid suspected of containing an analyte with soybean peroxidase or a soybean peroxidase-labeled antibody, an indicator which is capable of providing a detectable change in the presence of peroxidase and hydrogen peroxide, and determining said detectable change as a result of the presence of said analyte.
Claim 9
Independent Claim
63 words
A method for the determination of an analyte comprising the steps of contacting a sample of a liquid suspected of containing an analyte with soybean peroxidase or a soybean peroxidase-labeled antibody, an indicator which is capable of providing a detectable change in the presence of peroxidase and hydrogen peroxide, and determining said detectable change as a result of the presence of said analyte.
Claim 10
Dependent Claim
37 words
The method of claim 6 for the determination of an analyte other than hydrogen peroxide wherein said sample is also contacted with an interactive composition which is capable of reacting with said analyte to provide hydrogen peroxide.
Claim 11
Independent Claim
83 words
A method for detecting an antigen which includes the steps of adding soybean peroxidase to a sample suspected of containing the antigen to be detected under conditions such that said peroxidase directly or indirectly bonds to said antigen via an antibody and adding a substrate for said peroxidase and an indicator to said sample under conditions such that said peroxidase and said substrate react with said indicator and produce a change which is an indication of the presence and/or amount of said antigen.
Claim 12
Independent Claim
83 words
A method for detecting an antigen which includes the steps of adding soybean peroxidase to a sample suspected of containing the antigen to be detected under conditions such that said peroxidase directly or indirectly bonds to said antigen via an antibody and adding a substrate for said peroxidase and an indicator to said sample under conditions such that said peroxidase and said substrate react with said indicator and produce a change which is an indication of the presence and/or amount of said antigen.
Claim 13
Dependent Claim
12 words
The method of claim 8 wherein said indicator is a leuco dye.
Claim 14
Dependent Claim
13 words
The method of claim 8 wherein said peroxidase is conjugated with said antibody.
Claim 15
Dependent Claim
30 words
The method of claim 8 wherein said method employs a first antibody which is specific to said antigen and a second antibody which bridges said first antibody to said peroxidase.
Claim 16
Dependent Claim
4 words
A soybean peroxidase-antibody conjugate.
Claim 17
Dependent Claim
4 words
A soybean peroxidase-antibody conjugate.
Claim 18
Dependent Claim
6 words
A soybean peroxidase-antisoybean peroxidase antibody complex.
Claim 19
Dependent Claim
6 words
A soybean peroxidase-antisoybean peroxidase antibody complex.
References Cited (Backward Citations)
US5147793A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-01-10 | 1997-07-12 | 1998-01-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-01-10 | 2001-07-12 | 2002-01-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-01-10 | 2005-07-12 | 2006-01-11 | $7700.00 | Window Closed / Lapsed |