US5270197A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Cells expressing a substantial number of surface high affinity HBGF receptors but relatively few low affinity HBGF binding sites and system for assaying binding to HBGF receptor
Assignee: Boston Children's Hospital
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Filing: 1990-12-20
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Granted: 1993-12-14
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Expires: 2010-12-20
Abstract
Abstract A homogeneous population of cells having on average (1) a number of cell surface low-affinity heparin-binding growth factor (HBGF) sites per cell less than 20% of the number of such binding sites found on wild-type CHO-K1 cells (ATCC Accession No. CCL61), and at least three times the number of cell surface high-affinity HBGF receptors per cell found on such CHO-K1 cells; and an assay system utilizing such cells.
Inventors
- 👤 Avner Yayon
- 👤 David M. Ornitz
- 👤 Michael Klagsbrun
- 👤 Philip Leder
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 20:46
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/5005; G01N33/5008; C; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/50; C07K14/503; C07K14/705; C07K14/71; C12; C12N; C12N9/00; C12N9/14; C12N9/16; G01N33/74; C07K2319/00; C07K2319/01; C07K2319/034; C07K2319/32; C07K2319/61; G01N2500/00
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
62 words
A homogeneous in vitro population of mammalian cells having on average a number of cell surface low-affinity HBGF-binding sites per cell less than 20% of the number of such binding sites found on wild-type CHO-K1 cells (ATCC Accession No. CCL61), and on average at least three times the number of cell surface high-affinity HBGF receptors per cell found on said CHO-K1 cells.
Claim 2
Dependent Claim
62 words
A homogeneous in vitro population of mammalian cells having on average a number of cell surface low-affinity HBGF-binding sites per cell less than 20% of the number of such binding sites found on wild-type CHO-K1 cells (ATCC Accession No. CCL61), and on average at least three times the number of cell surface high-affinity HBGF receptors per cell found on said CHO-K1 cells.
Claim 3
Dependent Claim
25 words
The population of cells of claim 1 wherein each of the cells in said population comprises a recombinant nucleic acid encoding said high-affinity HBGF receptor.
Claim 4
Dependent Claim
27 words
The population of cells of claim 1, wherein said high-affinity HBGF receptor is endogenous to a species selected from the group consisting of human, mouse, and chicken.
Claim 5
Dependent Claim
18 words
The population of cells of claim 3, wherein said high-affinity HBGF receptor is a mouse high-affinity HBGF receptor.
Claim 6
Dependent Claim
19 words
The population of cells of claim 1, wherein said high-affinity HBGF receptor is a high-affinity aFGF or bFGF receptor.
Claim 7
Dependent Claim
23 words
The population of cells of claim 2, wherein said recombinant nucleic acid is incorporated into the genome of each cell of said population.
Claim 8
Dependent Claim
24 words
The population of cells of claim 2, wherein each cell of said population is transiently transfected with a vector comprising said recombinant nucleic acid.
Claim 9
Dependent Claim
57 words
A system for assaying the ability of a substance to bind to a high-affinity HBGF receptor, which system comprises a cell of the population of cells of claim 1 (the "test cell"), and an amount of heparin or a heparin-like molecule sufficient to induce binding of a HBGF to a high-affinity HBGF receptor on said test cell.
Claim 10
Dependent Claim
43 words
A system for assaying the ability of a substance to affect the interaction between a given type of HBGF and a high-affinity HBGF receptor, which system comprises said given type of HBGF and a cell of the population of cells of claim 1.
References Cited (Backward Citations)
US4882275A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-12-13 | 1997-06-14 | 1997-12-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-12-13 | 2001-06-14 | 2001-12-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-12-13 | 2005-06-14 | 2005-12-14 | $7700.00 | Window Closed / Lapsed |