US5268306A
Expired / Lapsed
US
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Preparation of a solid phase matrix containing a bound specific binding pair
Assignee: Roche Diagnostics GmbH
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Filing: 1991-12-23
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Granted: 1993-12-07
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Expires: 2011-12-23
Abstract
Abstract A specific binding pair is bound to an insoluble carrier for use in determining an analyte such as in an immunoassay. The carrier is coated with a first polymer containing a protein polymer having a molecular weight of at least about 20,000 and molecules of a first member of a specific binding pair. A second polymer containing a second member of the specific binding pair is bound to the first member on the carrier by binding of the first and second members of the specific binding pair. The first polymer is preferably more hydrophobic than the second polymer. The protein polymer can be prepared by cross-linking hydrophobic protein molecules of 10,000 to 700,000 molecular weight with a bifunctional or polyfunctional compound to obtain a protein polymer of 200,000 to 20,000,000 molecular weight. The second polymer can be the second member of the specific binding pair or the second member cross-linked with a linker or the second member cross-linked to a hydrophobic protein.
Inventors
- 👤 Michael Berger
- 👤 Arno Deger
- 👤 Josef Maier
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 16:15
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/543; G01N33/54353; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/811; Y10S530/812; Y10S530/813; Y10S530/815; Y10S530/816
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
157 words
Process for the production of a solid phase matrix of an insoluble carrier with a specifically bindable substance bound thereto, comprising: (i) coating a substance on an insoluble carrier material, wherein said substance contains a first polymer, said first polymer is a protein polymer comprising hydrophobic protein molecules having a molecular weight of from 10,000 to 700,000 which have been cross-linked with a bifunctional or polyfunctional compound to produce said protein polymer, and a plurality of molecules of a first member of a specific binding pair P1, and (ii) cross linking said first polymer to a second polymer which comprises a plurality of molecules of a second member of a specific binding pair P2, and binding sites for a complex of an immune component and an analyte, wherein said substance and said second polymer are cross linked via binding of P1 and P2 to each other, wherein said first polymer is more hydrophobic than said second polymer.
Claim 2
Dependent Claim
157 words
Process for the production of a solid phase matrix of an insoluble carrier with a specifically bindable substance bound thereto, comprising: (i) coating a substance on an insoluble carrier material, wherein said substance contains a first polymer, said first polymer is a protein polymer comprising hydrophobic protein molecules having a molecular weight of from 10,000 to 700,000 which have been cross-linked with a bifunctional or polyfunctional compound to produce said protein polymer, and a plurality of molecules of a first member of a specific binding pair P1, and (ii) cross linking said first polymer to a second polymer which comprises a plurality of molecules of a second member of a specific binding pair P2, and binding sites for a complex of an immune component and an analyte, wherein said substance and said second polymer are cross linked via binding of P1 and P2 to each other, wherein said first polymer is more hydrophobic than said second polymer.
Claim 3
Dependent Claim
30 words
Process of claim 1, wherein said protein is a soluble protein having a molecular weight above about 200,000 and is more hydrophobic than both P 1 and P 2 .
Claim 4
Dependent Claim
17 words
Process of claim 1, wherein said protein polymer has a molecular weight of from 200,000 to 20,000,000.
Claim 5
Dependent Claim
11 words
Process of claim 1, wherein said bifunctional compound is disuccinimidyl suberate.
Claim 6
Dependent Claim
14 words
Process of claim 1, wherein said protein is bovine serum albumin or immune-gamma globulin.
Claim 7
Dependent Claim
22 words
Process of claim 1, wherein said second polymer consists of cross-linked molecules of P 2 and a homo-, heterobi- or polyfunctional linker.
Claim 8
Dependent Claim
15 words
Process of claim 1, wherein said second polymer consists of molecules of P 2 .
Claim 9
Dependent Claim
17 words
Process of claim 1, wherein said second polymer comprises a second component cross-linked with P 2 .
Claim 10
Dependent Claim
12 words
Process of claim 1, wherein said second polymer comprises a hydrophobic protein.
Claim 11
Dependent Claim
17 words
Process of claim 1, wherein said second polymer comprises a hydrophobic protein cross-linked to P 2 .
Claim 12
Dependent Claim
27 words
Process of claim 1, wherein the specific binding pair of P 1 -P 2 is selected from the group consisting of biotin-avidin, biotin-streptavidin, antigen-antibody, hapten-antibody, protein A-immune-γ-globulin.
Claim 13
Dependent Claim
22 words
Process according to claim 1, wherein said insoluble solid carrier is furan, glass, titanium, dioxide, polystyrene, γ-globulin activated polystyrene, paper, or polyolefin.
Claim 14
Dependent Claim
17 words
Solid matrix useful in determining an analyte in a sample prepared by the process of claim 1.
References Cited (Backward Citations)
EP0016552A1
Cited
US4282287A
Cited
GB2103791A
Cited
EP0122209A1
Cited
US4820644A
Cited
WO1987004794A1
Cited
US4778751A
Cited
EP0269092A2
Cited
US5061640A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-12-06 | 1997-06-07 | 1997-12-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-12-06 | 2001-06-07 | 2001-12-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-12-06 | 2005-06-07 | 2005-12-07 | $7700.00 | Window Closed / Lapsed |