US5268290A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for producing neuraminidase
Assignee: KH Neochem Co Ltd
•
Filing: 1989-07-25
•
Granted: 1993-12-07
•
Expires: 2009-07-25
Abstract
Abstract Streptomyces lividans transformed with a recombinant DNA containing a DNA fragment which is derived from Micromonospora viridifaciens and which comprises a DNA sequence encoding a neuraminidase as well as flanking sequence regions is cultured, and neuraminidase can be efficiently recovered from the culture. Neuraminidase is utilized for clinical diagnosis of inflammatory diseases, diffuse collagen diseases, cancer, etc.
Inventors
- 👤 Mamoru Hasegawa
- 👤 Kazuhiro Sakurada
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 02:11
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12Y; C12Y302/00; C12Y302/01; C12Y302/01018; A; A61; A61K; A61K38/00
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
80 words
A process for producing neuraminidase which comprises culturing in a medium a microorganism belonging to the genus Streptomyces and transformed with a recombinant DNA containing an isolated DNA fragment that consists essentially of a M. viridifaciens DNA fragment comprising a DNA sequence encoding a neuraminidase and no more than 500 base pairs of native 3'-flanking sequence regions, and no more than 5.2K base pairs of native 5'-flanking sequence regions, accumulating neuraminidase in a culture and recovering neuraminidase from the culture.
Claim 2
Dependent Claim
80 words
A process for producing neuraminidase which comprises culturing in a medium a microorganism belonging to the genus Streptomyces and transformed with a recombinant DNA containing an isolated DNA fragment that consists essentially of a M. viridifaciens DNA fragment comprising a DNA sequence encoding a neuraminidase and no more than 500 base pairs of native 3'-flanking sequence regions, and no more than 5.2K base pairs of native 5'-flanking sequence regions, accumulating neuraminidase in a culture and recovering neuraminidase from the culture.
Claim 3
Dependent Claim
26 words
The process according to claim 1, wherein the microorganism belonging to the genus Streptomyces is Streptomyces lividans NED-BG6 (FERM BP-1768) or Streptomyces lividans NED-BC4 (FERM BP-2340).
Claim 4
Dependent Claim
58 words
A microorganism belonging to the genus Streptomyces and transformed with a recombinant DNA containing an isolated DNA fragment that consists essentially of a M. viridifaciens DNA fragment comprising a DNA sequence encoding a neuraminidase and no more than 500 base pairs of native 3'-flanking sequence regions, and no more than 5.2K base pairs of native 5'-flanking sequence regions.
Claim 5
Dependent Claim
58 words
A microorganism belonging to the genus Streptomyces and transformed with a recombinant DNA containing an isolated DNA fragment that consists essentially of a M. viridifaciens DNA fragment comprising a DNA sequence encoding a neuraminidase and no more than 500 base pairs of native 3'-flanking sequence regions, and no more than 5.2K base pairs of native 5'-flanking sequence regions.
Claim 6
Dependent Claim
19 words
The microorganism according to claim 3, which is Streptomyces lividans NED-BG6 (FERM BP-1768) or Streptomyces lividans NED-BC4 (FERM BP-2340).
Claim 7
Dependent Claim
48 words
A recombinant DNA containing an isolated DNA fragment that consists essentially of a DNA fragment comprising a M. viridifaciens DNA sequence encoding a neuraminidase and no more than 500 base pairs of native 3'-flanking sequence regions, and no more than 5.2K base pairs of native 5'-flanking sequence regions.
Claim 8
Dependent Claim
48 words
A recombinant DNA containing an isolated DNA fragment that consists essentially of a DNA fragment comprising a M. viridifaciens DNA sequence encoding a neuraminidase and no more than 500 base pairs of native 3'-flanking sequence regions, and no more than 5.2K base pairs of native 5'-flanking sequence regions.
Claim 9
Dependent Claim
70 words
Recombinant DNA pNED-BG6 containing a DNA fragment which comprises a DNA sequence encoding a neuraminidase as well as flanking sequence regions, characterized in that it is 8.3 kb, and has the following numbers of restriction endonuclease cleavage sites, 2 for Bam HI, 3 for Xho I, 5 for Bcl I, 3 for Cla I, 1 for Sac I, 2 for Bgl II, 2 for Kpn and 1 for Pst I.
Claim 10
Dependent Claim
70 words
Recombinant DNA pNED-BG6 containing a DNA fragment which comprises a DNA sequence encoding a neuraminidase as well as flanking sequence regions, characterized in that it is 8.3 kb, and has the following numbers of restriction endonuclease cleavage sites, 2 for Bam HI, 3 for Xho I, 5 for Bcl I, 3 for Cla I, 1 for Sac I, 2 for Bgl II, 2 for Kpn and 1 for Pst I.
Claim 11
Dependent Claim
70 words
Recombinant DNA pNED-BC6 containing a DNA fragment which comprises a DNA sequence encoding a neuraminidase as well as flanking sequence regions, characterized in that it is 13.7 kb, and has the following numbers of restriction endonuclease cleavage sites, 3 for Bam HI, 3 for Xho I, 5 for Bcl I, 3 for Cla I, 1 for Sac I, 2 for Bgl II, 2 for Kpn and 3 for Pst I.
Claim 12
Dependent Claim
70 words
Recombinant DNA pNED-BC6 containing a DNA fragment which comprises a DNA sequence encoding a neuraminidase as well as flanking sequence regions, characterized in that it is 13.7 kb, and has the following numbers of restriction endonuclease cleavage sites, 3 for Bam HI, 3 for Xho I, 5 for Bcl I, 3 for Cla I, 1 for Sac I, 2 for Bgl II, 2 for Kpn and 3 for Pst I.
Claim 13
Dependent Claim
28 words
A gene encoding neuraminidase derived from a microorganism belonging to the genus Micromonospora represented by the DNA nucleotide sequence coding for the following amino acid sequence. ##STR2## ##STR3##
Claim 14
Dependent Claim
28 words
A gene encoding neuraminidase derived from a microorganism belonging to the genus Micromonospora represented by the DNA nucleotide sequence coding for the following amino acid sequence. ##STR2## ##STR3##
Claim 15
Dependent Claim
24 words
The process according to claim 1, wherein the isolated DNA fragment contains the nucleotide sequence coding for the following amino acid sequence. ##STR4## ##STR5##
Claim 16
Dependent Claim
25 words
The recombinant isolated DNA according to claim 5, wherein the DNA fragment contains the nucleotide sequence coding for the following amino acid sequence. ##STR6## ##STR7##
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-12-06 | 1997-06-07 | 1997-12-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-12-06 | 2001-06-07 | 2001-12-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-12-06 | 2005-06-07 | 2005-12-07 | $7700.00 | Window Closed / Lapsed |