US5266199A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Serum separating apparatus
Assignee: Niigata Engineering Co Ltd
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Filing: 1991-11-19
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Granted: 1993-11-30
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Expires: 2011-11-19
Abstract
Abstract This invention relates to an apparatus used in a centrifugal separating step for sample preparation in the field of clinical chemistry. Especially, the invention relates to a separating apparatus used for isolating a serum fraction from a freshly isolated blood sample without contamination with a clot fraction. A serum separating apparatus according to the present invention is characterized by comprising an elastic body in the form of a cylindrical tube and a ball which can fitted to an opening of the elastic body and by positioning at a boundary of said fractions after the centrifugation.
Inventors
- 👤 Youichi Tsukagoshi
- 👤 Toshio Inoue
- 👤 Toshimasa Yamamoto
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 21:20
CPC Classifications
B; B01; B01L; B01L3/00; B01L3/50; B01L3/502; B01L3/5021; B01L3/50215; G; G01; G01N; G01N33/00; G01N33/48; G01N33/483; G01N33/487; G01N33/49; G01N33/491
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
159 words
A serum separating apparatus used for separating serum and clot fractions in a step of centrifuging to blood sample, said serum separating apparatus comprising: an elastic ring have a specific gravity of between 1.05 and 1.5: an elastic cylindrical tube being flexible and having top and bottom elastic flanges, the top and bottom flanges extending horizontally from the surface of said tube and having a specific gravity of between 1.05 and 1.5, said elastic cylindrical tube being surrounded by said elastic ring; and a ball having a diameter in the range of 50 to 90% of the inner diameter of said tube and having a specific gravity less than 1.03, which is fitted to an opening of said elastic cylindrical tube, wherein the specific gravity of said ball is less than that of serum, and the mean specific gravity of said elastic tube, said elastic ring, and said ball is in the range between that of serum and clot.
Claim 2
Dependent Claim
159 words
A serum separating apparatus used for separating serum and clot fractions in a step of centrifuging to blood sample, said serum separating apparatus comprising: an elastic ring have a specific gravity of between 1.05 and 1.5: an elastic cylindrical tube being flexible and having top and bottom elastic flanges, the top and bottom flanges extending horizontally from the surface of said tube and having a specific gravity of between 1.05 and 1.5, said elastic cylindrical tube being surrounded by said elastic ring; and a ball having a diameter in the range of 50 to 90% of the inner diameter of said tube and having a specific gravity less than 1.03, which is fitted to an opening of said elastic cylindrical tube, wherein the specific gravity of said ball is less than that of serum, and the mean specific gravity of said elastic tube, said elastic ring, and said ball is in the range between that of serum and clot.
Claim 3
Dependent Claim
84 words
A serum separating apparatus according to claim 1, wherein said elastic tube is made from a non-thrombotic and biologically inactive material, the non-thrombotic and biologically inactive material having the characteristic of preventing the blood sample from adhering thereto, wherein said material is selected from a group consisting of ABS resin, polyethylene terephtalate resin, polybutylene terephtalate resin, polycarbonate resin, polyamide resin, polymethylmethacrylate resin, and polyacetal resin, and wherein said ball is made of a plastic material selected from a group consisting of polystyrene and polypropylene.
Claim 4
Dependent Claim
19 words
A serum separating apparatus according to claim 1, wherein said elastic ring has an opening providing two horizontal ends.
Claim 5
Dependent Claim
34 words
A serum separating apparatus according to claim 3, wherein said two horizontal ends of the elastic ring respectively have an L-shape and a complementary reversed L-shape so as to interlock when meeting each other.
Claim 6
Dependent Claim
19 words
A serum separating apparatus according to claim 1, wherein said elastic ring has greater flexibility than said elastic tube.
Claim 7
Dependent Claim
32 words
A serum separating apparatus according to claim 5, wherein said elastic ring is made from one of derivatives of plant material including cellulose fibers and derivatives of animal material including silk fibers.
Claim 8
Dependent Claim
127 words
A serum separating apparatus used for dividing serum and clot fractions in a step of centrifuging a blood sample, said serum separating apparatus comprising: an elastic ring have a specific gravity of between 1.03 and 1.5: an elastic cylindrical tube having top and bottom elastic flanges, the top and bottom flanges extending horizontally from the surface of said elastic tube and having a specific gravity of between 1.03 and 1.5, the elastic tube being surrounded by said elastic ring; and a ball having a diameter in the range of at least 35% of the inner diameter of said tube and having a specific gravity which is less than 1.05, which is fitted to an opening of said elastic tube; wherein said elastic ring has two horizontal ends.
Claim 9
Dependent Claim
127 words
A serum separating apparatus used for dividing serum and clot fractions in a step of centrifuging a blood sample, said serum separating apparatus comprising: an elastic ring have a specific gravity of between 1.03 and 1.5: an elastic cylindrical tube having top and bottom elastic flanges, the top and bottom flanges extending horizontally from the surface of said elastic tube and having a specific gravity of between 1.03 and 1.5, the elastic tube being surrounded by said elastic ring; and a ball having a diameter in the range of at least 35% of the inner diameter of said tube and having a specific gravity which is less than 1.05, which is fitted to an opening of said elastic tube; wherein said elastic ring has two horizontal ends.
Claim 10
Dependent Claim
34 words
A serum separating apparatus according to claim 7, wherein said two horizontal ends of the elastic ring respectively have an L-shaped and a complementary reversed L-shape so as to interlock when meeting each other.
Claim 11
Dependent Claim
90 words
A serum separating apparatus according to claim 7, wherein said elastic tube and said elastic ring are made of a non-thrombotic and biologically inactive material, the non-thrombotic and biologically inactive material having the characteristic of preventing the blood sample from adhering thereto, wherein said material is selected from a group consisting of ABS resin, polyethylene terephtalate resin, polyurethane resin, polybutylene terephtalate resin, polycarbonate resin, polyamide resin, polymethylmethacrylate resin, and polyacetal resin, and wherein said ball is made of a plastic material selected from a group consisting of polystyrene and polypropylene.
Claim 12
Dependent Claim
33 words
A serum separating apparatus according to claim 7, wherein said elastic tube has an opening through one end to another with a gradient of inner diameters along the longitudinal axis of said tube.
Claim 13
Dependent Claim
30 words
A serum separating apparatus according to claim 10, wherein an upper end of said opening is widely opened to prevent accumulation of clot or platelet adhesion on said elastic body.
Claim 14
Dependent Claim
145 words
A serum separating apparatus used for dividing serum and clot fractions in a step of centrifuging a blood sample, said serum separating apparatus comprising: an elastic ring have a specific gravity of between 1.03 and 1.5: an elastic cylindrical tube having a specific gravity of between 1.03 and 1.5, the elastic ring surrounding said elastic cylindrical tube which is provided within an inner space, the elastic cylindrical tube having a supporting entity so as to be fixed with the elastic ring; and a ball having a specific gravity less than 1.05 and a diameter in the range 35% or more of the inner diameter of said tube; wherein an upper inner side of said elastic cylindrical tube has at least a pair of projected portions capable of being horizontally extended from said elastic tube so as to prevent passing of the ball through the tube.
Claim 15
Dependent Claim
145 words
A serum separating apparatus used for dividing serum and clot fractions in a step of centrifuging a blood sample, said serum separating apparatus comprising: an elastic ring have a specific gravity of between 1.03 and 1.5: an elastic cylindrical tube having a specific gravity of between 1.03 and 1.5, the elastic ring surrounding said elastic cylindrical tube which is provided within an inner space, the elastic cylindrical tube having a supporting entity so as to be fixed with the elastic ring; and a ball having a specific gravity less than 1.05 and a diameter in the range 35% or more of the inner diameter of said tube; wherein an upper inner side of said elastic cylindrical tube has at least a pair of projected portions capable of being horizontally extended from said elastic tube so as to prevent passing of the ball through the tube.
Claim 16
Dependent Claim
28 words
A serum separating apparatus according to claim 12, wherein the bottom of the elastic body has an elastic flange which being horizontally extended to support the elastic ring.
Claim 17
Dependent Claim
22 words
A serum separating apparatus according to claim 12, wherein said elastic ring is an opened ring having first and second horizontal ends.
Claim 18
Dependent Claim
27 words
A serum separating apparatus according to claim 12, wherein said first and second horizontal ends are L-shape and reversed L-shape, respectively, so as to meet each other.
Claim 19
Dependent Claim
85 words
A serum separating apparatus according to claim 12, wherein said elastic body and said elastic ring are made of a non-thrombotic and biologically inactive material, which are difficult to be adhered by the blood sample, wherein said material is selected from a group consisting of ABS resin, polyethylene terephtalate resin, polyurethane resin, polybuthylene terephtalate resin, polycarbonate resin, polyamide resin, polymethylmethacrylate resin and polyacetal resin, and wherein said ball is made of a material selected from a group of plastic materials such as polystyrene and polypropylene.
Claim 20
Dependent Claim
140 words
A serum separating apparatus used for separating serum and clot fractions during centrifugation of a blood sample, said serum separating apparatus comprising: an elastic ring having a specific gravity of between 1.03 and 1.5: an elastic cylindrical tube having a specific gravity of between 1.03 and 1.5, surrounded by said elastic ring, said elastic cylindrical tube having top and bottom elastic flanges capable of horizontally extending from the surface of said elastic tube; and a ball having a diameter in the range of 50% to 90% of the inner diameter of said elastic tube, said ball being fit within an opening of said elastic body, wherein the specific gravity of said ball is less than 1.03, and the mean specific gravity of said elastic tube, said elastic ring, and said ball is in the range of between 1.03 and 1.08.
Claim 21
Dependent Claim
140 words
A serum separating apparatus used for separating serum and clot fractions during centrifugation of a blood sample, said serum separating apparatus comprising: an elastic ring having a specific gravity of between 1.03 and 1.5: an elastic cylindrical tube having a specific gravity of between 1.03 and 1.5, surrounded by said elastic ring, said elastic cylindrical tube having top and bottom elastic flanges capable of horizontally extending from the surface of said elastic tube; and a ball having a diameter in the range of 50% to 90% of the inner diameter of said elastic tube, said ball being fit within an opening of said elastic body, wherein the specific gravity of said ball is less than 1.03, and the mean specific gravity of said elastic tube, said elastic ring, and said ball is in the range of between 1.03 and 1.08.
Claim 22
Dependent Claim
141 words
A serum separating apparatus used for separating serum and clot fractions during centrifugation of a blood sample, said serum separating apparatus comprising; an elastic ring having a specific gravity of between 1.05 and 1.5; an elastic cylindrical tube having top and bottom elastic flanges extending horizontally from the surface of said elastic tube, the elastic tube having a specific gravity of between 1.05 and 1.5, the elastic ring surrounding at least a portion of the elastic tube; and a ball having a diameter int he range of 50% to 90% of the inner diameter of said elastic tube and having a specific gravity which is at most equal to 1.03, the ball being fit to an opening of said elastic tube, wherein said elastic material used for the elastic ring is more flexible than the material used for the elastic tube.
Claim 23
Dependent Claim
141 words
A serum separating apparatus used for separating serum and clot fractions during centrifugation of a blood sample, said serum separating apparatus comprising; an elastic ring having a specific gravity of between 1.05 and 1.5; an elastic cylindrical tube having top and bottom elastic flanges extending horizontally from the surface of said elastic tube, the elastic tube having a specific gravity of between 1.05 and 1.5, the elastic ring surrounding at least a portion of the elastic tube; and a ball having a diameter int he range of 50% to 90% of the inner diameter of said elastic tube and having a specific gravity which is at most equal to 1.03, the ball being fit to an opening of said elastic tube, wherein said elastic material used for the elastic ring is more flexible than the material used for the elastic tube.
Claim 24
Dependent Claim
30 words
A serum separating apparatus according to claim 18, wherein the material used for the elastic ring is one of plant material including cellulose fibers and animal material including silk fibers.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-11-29 | 1997-05-31 | 1997-11-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-11-29 | 2001-05-31 | 2001-11-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-11-29 | 2005-05-31 | 2005-11-30 | $7700.00 | Window Closed / Lapsed |