US5246846A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for immobilizing proteins on a support containing amino, mercapto or hydroxy groups
Assignee: Individual
•
Filing: 1990-04-04
•
Granted: 1993-09-21
•
Expires: 2010-04-04
Abstract
Abstract Reactants containing amino, mercapto or hydroxy groups such as proteins, peptides, ligands, coenzymes or enzymes are immobilized on a support containing amino, mercapto or hydroxy groups by coupling the reactant to the support with a compound having the following formula (I) or (IA): ##STR1## wherein X is a halogen and R 1 , R 2 are the same or different and are X, R, COR, COOR, wherein R is C 1 -C 8 alkyl, COOH, CNS, N 3 or CN. The support may be first reacted with the compound to produce a derivatized support which is then reacted the reactant or the reactant may be first reacted with the compound and the resultant product then reacted with the support. An electrochemical biosensor can be prepared by using a conductive support.
Inventors
- 👤 Fritz Pittner
- 👤 Thomas Schalkhammer
- 👤 Gerald Urban
- 👤 Eva Mann-Buxbaum
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 08:19
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/002; C12Q1/003; C12N; C12N11/00; C12N11/02; C12N11/06; C12N11/14; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/811; Y10S530/812; Y10S530/815; Y10S530/816
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
109 words
A process for immobilizing a reactant on a support having amino, mercapto or hydroxy groups comprising (i) reacting a support having an amino, mercapto or hydroxy group with a compound having the formula (I) or (IA) ##STR5## wherein X is a halogen and R 1 , R 2 are the same or different and are X, R, COR, COOR, wherein R is C 1 -C 8 alkyl, COOH, CNS, N 3 or CN to form a derivatized support, and (ii) reacting a reactant to be immobilized having an amino, mercapto or hydroxy group with the halogen in ortho or para position to the C═O group of said derivatized support.
Claim 2
Dependent Claim
109 words
A process for immobilizing a reactant on a support having amino, mercapto or hydroxy groups comprising (i) reacting a support having an amino, mercapto or hydroxy group with a compound having the formula (I) or (IA) ##STR5## wherein X is a halogen and R 1 , R 2 are the same or different and are X, R, COR, COOR, wherein R is C 1 -C 8 alkyl, COOH, CNS, N 3 or CN to form a derivatized support, and (ii) reacting a reactant to be immobilized having an amino, mercapto or hydroxy group with the halogen in ortho or para position to the C═O group of said derivatized support.
Claim 3
Dependent Claim
14 words
A process according to claim 1, wherein said compound of formula (I) is 2,3,5,6-tetrachlorocyclohexadien-1,4-dione.
Claim 4
Dependent Claim
16 words
A process according to claim 1, wherein said compound of formula (IA) is 3,4,5,6-tetrachlorocyclohexadien-1,2-dione or 3,4,5,6-tetrabromocyclohexadien-1,2-dione.
Claim 5
Dependent Claim
12 words
A process according to claim 1, wherein said reactant is a protein.
Claim 6
Dependent Claim
12 words
A process according to claim 1, wherein said reactant is a ligand.
Claim 7
Dependent Claim
12 words
A process according to claim 1, wherein said reactant is a coenzyme.
Claim 8
Dependent Claim
13 words
A process according to claim 1, wherein said reactant is a redox enzyme.
Claim 9
Dependent Claim
16 words
A process as in claim 1, wherein said derivatized support is dried prior to step (ii).
Claim 10
Dependent Claim
18 words
A process according to claim 1, wherein step (i) is performed in organic or organically aqueous mixed phases.
Claim 11
Dependent Claim
15 words
A process according to claim 9, wherein step (i) is performed in anhydrous organic solvents.
Claim 12
Dependent Claim
19 words
A process according to claim 1, wherein step (i) is performed at temperatures between -10° C. and reflux temperature.
Claim 13
Dependent Claim
31 words
A process according to claim 1, wherein said reactant is a redoxenzyme selected from the group consisting of glucoseoxidase, galactoseoxidase, amino acid oxidase, xanthinoxidase, cholesteroloxidase, uricase and ascorbatoxidase and their apoenzymes.
Claim 14
Dependent Claim
14 words
A process according to claim 1, wherein said reactant is an antigen or antibody.
Claim 15
Dependent Claim
26 words
A process according to claim 1, wherein said reactant is a naringinase, alpha- or beta-glucosidase, glycosidase, galactosidase, lipase, pectinase, esterase, penicillininase, ammonialysase, urease or a phosphatase.
Claim 16
Dependent Claim
33 words
A process according to claim 1, further comprising after step (i) partially deactivating the surface of said derivatized support by photochemical reaction or by use of alkaline reagents or low-molecular amines or thiols.
Claim 17
Dependent Claim
59 words
A process according to claim 1, further comprising after step (ii) carrying out the steps of (iii) reacting the product of step ii) with said compound, and (iv) reacting a reactant to be immobilized having an amine, mercapto or hydroxy group with the halogen in ortho or para position to the C═O group of the product of step iii).
Claim 18
Dependent Claim
106 words
A process for immobilizing a reactant on a support having amino, mercapto or hydroxy groups comprising (i) reacting a reactant to be immobilized having an amino, mercapto or hydroxy group with a compound having the formula (I) or (IA) ##STR6## wherein X is a halogen and R 1 , R 2 are the same or different and are X, R, COR, COOR, wherein R is C 1 -C 8 alkyl, COOH, CNS, N 3 or CN, and (ii) reacting a support having an amine, mercapto or hydroxy group with the halogen in ortho or para position to the C═O group of the product of step i).
Claim 19
Dependent Claim
106 words
A process for immobilizing a reactant on a support having amino, mercapto or hydroxy groups comprising (i) reacting a reactant to be immobilized having an amino, mercapto or hydroxy group with a compound having the formula (I) or (IA) ##STR6## wherein X is a halogen and R 1 , R 2 are the same or different and are X, R, COR, COOR, wherein R is C 1 -C 8 alkyl, COOH, CNS, N 3 or CN, and (ii) reacting a support having an amine, mercapto or hydroxy group with the halogen in ortho or para position to the C═O group of the product of step i).
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-09-20 | 1997-03-22 | 1997-09-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-09-20 | 2001-03-22 | 2001-09-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-09-20 | 2005-03-22 | 2005-09-21 | $7700.00 | Window Closed / Lapsed |