US5243401A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Fluorescent image densitometer of flying spot system
Assignee: Shimadzu Corp
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Filing: 1992-01-30
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Granted: 1993-09-07
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Expires: 2012-01-30
Abstract
Abstract A two-dimensional fluorescent densitometry for measuring a specimen plate having a specimen spot such as phospholipide two-dimensionally developed and fluorescent-labelled on a TLC plate. The densitometer comprises an excitation optical system for scanning a measuring plate with an excitation light beam, a fluorescence detection system for detecting a fluorescence from the measuring plate, a correction table for storing fluorescent data together with positional information in a scanning direction on the measuring plate, and an arithmetic section for dividing fluorescence detection data by fluorescent data at the same position in the scanning direction on the measuring plate stored in the correction table.
Inventors
- 👤 Kazunari Sinya
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 15:58
CPC Classifications
G; G01; G01N; G01N30/00; G01N30/90; G01N30/95; G01N21/00; G01N21/62; G01N21/63; G01N21/64; G01N21/645; G01N21/6456; G01N30/02; G01N30/86; G01N30/8603; G01N30/8613
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
128 words
A fluorescent image densitometer of flying spot system, comprising: an excitation optical system for scanning and irradiating a measuring plate with an excitation light beam; a fluorescence detection system for detecting a fluorescence from an excitation light irradiation position of the measuring plate; a plate uniformly applied with a fluorescent agent at least within a range of excitation light; a correction table for storing fluorescent data, measured with said plate mounted as the measuring plate, together with information of a position in a scanning direction on the measuring plate; and an arithmetic section for dividing fluorescence detection data measured with a specimen plate mounted as the measuring plate by fluorescent data at the same position in the scanning direction on the measuring plate stored in said correction table.
Claim 2
Dependent Claim
128 words
A fluorescent image densitometer of flying spot system, comprising: an excitation optical system for scanning and irradiating a measuring plate with an excitation light beam; a fluorescence detection system for detecting a fluorescence from an excitation light irradiation position of the measuring plate; a plate uniformly applied with a fluorescent agent at least within a range of excitation light; a correction table for storing fluorescent data, measured with said plate mounted as the measuring plate, together with information of a position in a scanning direction on the measuring plate; and an arithmetic section for dividing fluorescence detection data measured with a specimen plate mounted as the measuring plate by fluorescent data at the same position in the scanning direction on the measuring plate stored in said correction table.
Claim 3
Dependent Claim
31 words
The fluorescent image densitometer according to claim 1, wherein the correction table for correcting a locality is prepared by measuring a TLC plate (thin film chromatography plate) with a fluorescent agent.
Claim 4
Dependent Claim
40 words
The fluorescent image densitometer according to claim 1, wherein the arithmetic section divides a fluorescent intensity when the specimen plate is measured by the value of the correction table at the same scanning position to obtain a locality-corrected fluorescent intensity.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-09-06 | 1997-03-08 | 1997-09-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-09-06 | 2001-03-08 | 2001-09-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-09-06 | 2005-03-08 | 2005-09-07 | $7700.00 | Window Closed / Lapsed |